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Seoul virus and hantavirus disease, Shenyang, People's Republic of China.

Zhang YZ, Dong X, Li X, Ma C, Xiong HP, Yan GJ, Gao N, Jiang DM, Li MH, Li LP, Zou Y, Plyusnin A - Emerging Infect. Dis. (2009)

Bottom Line: An outbreak of hemorrhagic fever with renal syndrome (HFRS) occurred among students in Shenyang Pharmaceutical University in 2006.We conducted a study to characterize etiologic agents of the outbreaks and clarify the origin of hantaviruses causing infections in humans and laboratory animals.Immunoglobulin (Ig) M or IgG antibodies against Seoul virus (SEOV) were detected in the serum samples of all 8 patients.

View Article: PubMed Central - PubMed

Affiliation: Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China. yongzhenzhang@sohu.com

ABSTRACT
An outbreak of hemorrhagic fever with renal syndrome (HFRS) occurred among students in Shenyang Pharmaceutical University in 2006. We conducted a study to characterize etiologic agents of the outbreaks and clarify the origin of hantaviruses causing infections in humans and laboratory animals. Immunoglobulin (Ig) M or IgG antibodies against Seoul virus (SEOV) were detected in the serum samples of all 8 patients. IgG antibodies against hantavirus were also identified in laboratory rats, which were used by these students for their scientific research. Phylogenetic analysis showed that partial small segment sequences recovered from humans, laboratory rats, and local wild rats belonged to SEOV. Hantavirus sequences recovered from humans and laboratory rats clustered within 1 of 3 lineages of SEOV circulating among local wild rats in Shenyang. These results suggest that the HFRS outbreak in Shenyang was caused by SEOV that was circulating among local wild rats and had also infected the laboratory rats.

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Related in: MedlinePlus

Detection of hantaviral antigens by indirect immunofluorescent assay. A) Hantaviral antigen- negative Rattus norvegicus lung tissue, detected with anti-L99 and 76-118 hantavirus sera. B) Hantaviral antigen-positive R. norvegicus lung tissue, detected with anti-L99 and 76-118 hantavirus antibodies. Magnification ×400.
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Figure 1: Detection of hantaviral antigens by indirect immunofluorescent assay. A) Hantaviral antigen- negative Rattus norvegicus lung tissue, detected with anti-L99 and 76-118 hantavirus sera. B) Hantaviral antigen-positive R. norvegicus lung tissue, detected with anti-L99 and 76-118 hantavirus antibodies. Magnification ×400.

Mentions: Viral antigens in the lung tissue (frozen sections) of rats and mice were detected by using indirect IFA as described previously (19), with rabbit anti-SEOV/L99 and HTNV/76–118 hantavirus antibodies and FITC-labeled goat antirabbit IgG (Sigma). Scattered, granular fluorescence in the cytoplasm was considered a positive reaction (Figure 1).


Seoul virus and hantavirus disease, Shenyang, People's Republic of China.

Zhang YZ, Dong X, Li X, Ma C, Xiong HP, Yan GJ, Gao N, Jiang DM, Li MH, Li LP, Zou Y, Plyusnin A - Emerging Infect. Dis. (2009)

Detection of hantaviral antigens by indirect immunofluorescent assay. A) Hantaviral antigen- negative Rattus norvegicus lung tissue, detected with anti-L99 and 76-118 hantavirus sera. B) Hantaviral antigen-positive R. norvegicus lung tissue, detected with anti-L99 and 76-118 hantavirus antibodies. Magnification ×400.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2662651&req=5

Figure 1: Detection of hantaviral antigens by indirect immunofluorescent assay. A) Hantaviral antigen- negative Rattus norvegicus lung tissue, detected with anti-L99 and 76-118 hantavirus sera. B) Hantaviral antigen-positive R. norvegicus lung tissue, detected with anti-L99 and 76-118 hantavirus antibodies. Magnification ×400.
Mentions: Viral antigens in the lung tissue (frozen sections) of rats and mice were detected by using indirect IFA as described previously (19), with rabbit anti-SEOV/L99 and HTNV/76–118 hantavirus antibodies and FITC-labeled goat antirabbit IgG (Sigma). Scattered, granular fluorescence in the cytoplasm was considered a positive reaction (Figure 1).

Bottom Line: An outbreak of hemorrhagic fever with renal syndrome (HFRS) occurred among students in Shenyang Pharmaceutical University in 2006.We conducted a study to characterize etiologic agents of the outbreaks and clarify the origin of hantaviruses causing infections in humans and laboratory animals.Immunoglobulin (Ig) M or IgG antibodies against Seoul virus (SEOV) were detected in the serum samples of all 8 patients.

View Article: PubMed Central - PubMed

Affiliation: Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China. yongzhenzhang@sohu.com

ABSTRACT
An outbreak of hemorrhagic fever with renal syndrome (HFRS) occurred among students in Shenyang Pharmaceutical University in 2006. We conducted a study to characterize etiologic agents of the outbreaks and clarify the origin of hantaviruses causing infections in humans and laboratory animals. Immunoglobulin (Ig) M or IgG antibodies against Seoul virus (SEOV) were detected in the serum samples of all 8 patients. IgG antibodies against hantavirus were also identified in laboratory rats, which were used by these students for their scientific research. Phylogenetic analysis showed that partial small segment sequences recovered from humans, laboratory rats, and local wild rats belonged to SEOV. Hantavirus sequences recovered from humans and laboratory rats clustered within 1 of 3 lineages of SEOV circulating among local wild rats in Shenyang. These results suggest that the HFRS outbreak in Shenyang was caused by SEOV that was circulating among local wild rats and had also infected the laboratory rats.

Show MeSH
Related in: MedlinePlus