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Exochelin production in Mycobacterium neoaurum.

Chan KG - Int J Mol Sci (2009)

Bottom Line: In this study, exochelin was purified from spent supernatant of M. neoaurum by semi-preparative chromatography.When saturated ferric chloride solution was added into the purified exochelin, a ferri-exochelin complex was formed.It is proposed that iron uptake in M. neoaurum is exochelin-mediated.

View Article: PubMed Central - PubMed

Affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, University of Malaya, Kuala Lumpur, Malaysia. kokgan@um.edu.my

ABSTRACT
Mycobacterium neoaurum is a soil saprophyte and obligate aerobic bacterium. This group of mycobacterium is relatively fast-growing. They form colonies on nutrient agar at 37 masculineC within 3 - 4 days. In natural soil habitats, bioavailability of iron is limited. To facilitate iron uptake, most mycobacteria produce siderophores. One example is exochelin, which is extracellular and water-soluble. In this report, the production of exochelin in M. neoaurum was induced in iron-deficiency, but repressed under ironsufficiency growth conditions. It is however not induced under zinc-deficiency growth conditions. The growth of this mycobacterium was correlated with exochelin secretion under iron-deficiency culture conditions. When M. neoaurum was grown in defined medium containing 0.04 microg Fe(III)/mL (final concentration), the production of exochelin reached a maximum and the corresponding cell growth was comparable to that under iron-sufficiency conditions. In this study, exochelin was purified from spent supernatant of M. neoaurum by semi-preparative chromatography. When saturated ferric chloride solution was added into the purified exochelin, a ferri-exochelin complex was formed. It is proposed that iron uptake in M. neoaurum is exochelin-mediated.

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Detection of M. neoaurum exochelin in spent supernatants of M. neoaurum grown in iron-deficiency, zinc-deficiency and iron-sufficiency.CAS assay of exochelin in spent supernatant of M. neoaurum grown in iron-deficiency (B), zincdeficiency (C) and iron-sufficiency (D) conditions as described in the Experimental Section (4.3). Control experiment (A) was carried out by mixing the uninoculated medium with CAS solution. Formation of yellow solution in CAS assay indicates presence of exochelin.
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f1-ijms-10-00345: Detection of M. neoaurum exochelin in spent supernatants of M. neoaurum grown in iron-deficiency, zinc-deficiency and iron-sufficiency.CAS assay of exochelin in spent supernatant of M. neoaurum grown in iron-deficiency (B), zincdeficiency (C) and iron-sufficiency (D) conditions as described in the Experimental Section (4.3). Control experiment (A) was carried out by mixing the uninoculated medium with CAS solution. Formation of yellow solution in CAS assay indicates presence of exochelin.

Mentions: M. neoaurum was cultured under iron-deficiency, zinc-deficiency and iron sufficiency conditions and its exochelin production assayed. Figure 1 illustrates the positive effect of iron-deficiency (0.02 μg Fe(III)/mL) on the production of exochelin in M. neoaurum. No detectable exochelin was observed in the spent supernatants obtained from both iron-sufficiency and zinc-deficiency conditions.


Exochelin production in Mycobacterium neoaurum.

Chan KG - Int J Mol Sci (2009)

Detection of M. neoaurum exochelin in spent supernatants of M. neoaurum grown in iron-deficiency, zinc-deficiency and iron-sufficiency.CAS assay of exochelin in spent supernatant of M. neoaurum grown in iron-deficiency (B), zincdeficiency (C) and iron-sufficiency (D) conditions as described in the Experimental Section (4.3). Control experiment (A) was carried out by mixing the uninoculated medium with CAS solution. Formation of yellow solution in CAS assay indicates presence of exochelin.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2662466&req=5

f1-ijms-10-00345: Detection of M. neoaurum exochelin in spent supernatants of M. neoaurum grown in iron-deficiency, zinc-deficiency and iron-sufficiency.CAS assay of exochelin in spent supernatant of M. neoaurum grown in iron-deficiency (B), zincdeficiency (C) and iron-sufficiency (D) conditions as described in the Experimental Section (4.3). Control experiment (A) was carried out by mixing the uninoculated medium with CAS solution. Formation of yellow solution in CAS assay indicates presence of exochelin.
Mentions: M. neoaurum was cultured under iron-deficiency, zinc-deficiency and iron sufficiency conditions and its exochelin production assayed. Figure 1 illustrates the positive effect of iron-deficiency (0.02 μg Fe(III)/mL) on the production of exochelin in M. neoaurum. No detectable exochelin was observed in the spent supernatants obtained from both iron-sufficiency and zinc-deficiency conditions.

Bottom Line: In this study, exochelin was purified from spent supernatant of M. neoaurum by semi-preparative chromatography.When saturated ferric chloride solution was added into the purified exochelin, a ferri-exochelin complex was formed.It is proposed that iron uptake in M. neoaurum is exochelin-mediated.

View Article: PubMed Central - PubMed

Affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, University of Malaya, Kuala Lumpur, Malaysia. kokgan@um.edu.my

ABSTRACT
Mycobacterium neoaurum is a soil saprophyte and obligate aerobic bacterium. This group of mycobacterium is relatively fast-growing. They form colonies on nutrient agar at 37 masculineC within 3 - 4 days. In natural soil habitats, bioavailability of iron is limited. To facilitate iron uptake, most mycobacteria produce siderophores. One example is exochelin, which is extracellular and water-soluble. In this report, the production of exochelin in M. neoaurum was induced in iron-deficiency, but repressed under ironsufficiency growth conditions. It is however not induced under zinc-deficiency growth conditions. The growth of this mycobacterium was correlated with exochelin secretion under iron-deficiency culture conditions. When M. neoaurum was grown in defined medium containing 0.04 microg Fe(III)/mL (final concentration), the production of exochelin reached a maximum and the corresponding cell growth was comparable to that under iron-sufficiency conditions. In this study, exochelin was purified from spent supernatant of M. neoaurum by semi-preparative chromatography. When saturated ferric chloride solution was added into the purified exochelin, a ferri-exochelin complex was formed. It is proposed that iron uptake in M. neoaurum is exochelin-mediated.

Show MeSH
Related in: MedlinePlus