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Compensatory growth induced in zebrafish larvae after pre-exposure to a Microcystis aeruginosa natural bloom extract containing microcystins.

Ghazali IE, Saqrane S, Carvalho AP, Ouahid Y, Oudra B, Del Campo FF, Vasconcelos V - Int J Mol Sci (2009)

Bottom Line: Early life stage tests with zebrafish (Danio rerio) were used to detect toxic effects of compounds from a Microcystis aeruginosa natural bloom extract on their embryolarval development.The specific growth of the pre-exposed larvae was significantly more important than that of the control larvae.This may represent a compensatory growth used to reduce the difference in size with the control fish noted after hatching.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, University Cadi Ayyad, Marrakech, Morocco. elghazali@gmail.com

ABSTRACT
Early life stage tests with zebrafish (Danio rerio) were used to detect toxic effects of compounds from a Microcystis aeruginosa natural bloom extract on their embryolarval development. We carried out the exposure of developing stages of fish to complex cyanobacterial blooms containing hepatotoxic molecules - microcystins. Fish embryo tests performed with the bloom extract containing 3 mg.L(-1) Eq microcystin-LR showed that after 24 h of exposure all fish embryos died. The same tests performed with other diluted extracts (containing 0.3, 0.1 and 0.03 mg.L(-1) Eq microcystin-LR) were shown to have an influence on zebrafish development and a large number of embryos showed malformation signs (edema, bent and curving tail). After hatching the larvae were transferred to a medium without toxins to follow the larval development under the new conditions. The specific growth of the pre-exposed larvae was significantly more important than that of the control larvae. This may represent a compensatory growth used to reduce the difference in size with the control fish noted after hatching.

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Related in: MedlinePlus

Localization of Lalla Takerkoust reservoir.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2662464&req=5

f5-ijms-10-00133: Localization of Lalla Takerkoust reservoir.

Mentions: The cyanobacterial bloom material was collected from Lalla Takerkoust reservoir (Figure 5) with a 27 μm phytoplankton net in September 2005. The bloom-forming species was identified as Microcystis aeruginosa by both microscopy and PCR analysis. The collected samples were freeze-dried and stored until total microcystins quantification.


Compensatory growth induced in zebrafish larvae after pre-exposure to a Microcystis aeruginosa natural bloom extract containing microcystins.

Ghazali IE, Saqrane S, Carvalho AP, Ouahid Y, Oudra B, Del Campo FF, Vasconcelos V - Int J Mol Sci (2009)

Localization of Lalla Takerkoust reservoir.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2662464&req=5

f5-ijms-10-00133: Localization of Lalla Takerkoust reservoir.
Mentions: The cyanobacterial bloom material was collected from Lalla Takerkoust reservoir (Figure 5) with a 27 μm phytoplankton net in September 2005. The bloom-forming species was identified as Microcystis aeruginosa by both microscopy and PCR analysis. The collected samples were freeze-dried and stored until total microcystins quantification.

Bottom Line: Early life stage tests with zebrafish (Danio rerio) were used to detect toxic effects of compounds from a Microcystis aeruginosa natural bloom extract on their embryolarval development.The specific growth of the pre-exposed larvae was significantly more important than that of the control larvae.This may represent a compensatory growth used to reduce the difference in size with the control fish noted after hatching.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, University Cadi Ayyad, Marrakech, Morocco. elghazali@gmail.com

ABSTRACT
Early life stage tests with zebrafish (Danio rerio) were used to detect toxic effects of compounds from a Microcystis aeruginosa natural bloom extract on their embryolarval development. We carried out the exposure of developing stages of fish to complex cyanobacterial blooms containing hepatotoxic molecules - microcystins. Fish embryo tests performed with the bloom extract containing 3 mg.L(-1) Eq microcystin-LR showed that after 24 h of exposure all fish embryos died. The same tests performed with other diluted extracts (containing 0.3, 0.1 and 0.03 mg.L(-1) Eq microcystin-LR) were shown to have an influence on zebrafish development and a large number of embryos showed malformation signs (edema, bent and curving tail). After hatching the larvae were transferred to a medium without toxins to follow the larval development under the new conditions. The specific growth of the pre-exposed larvae was significantly more important than that of the control larvae. This may represent a compensatory growth used to reduce the difference in size with the control fish noted after hatching.

Show MeSH
Related in: MedlinePlus