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Red blood cell glutathione peroxidase activity in female igravid and pregnant rats.

Gallo G, Martino G - Reprod. Biol. Endocrinol. (2009)

Bottom Line: Authors optimize the spectrophotometric method to measure glutathione peroxidase activity in rat red blood cell membranes.The optimization, when applied to age paired rats, both igravid and pregnant, shows that pregnancy induces, at seventeen d of pregnancy, an increase of both reactive oxygen substance concentration in red blood cells and membrane glutathione peroxidase activity.The glutathione peroxidase increase in erythrocyte membranes is induced by systemic oxidative stress long lasting rat pregnancy.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Cell Biology, University of Calabria, Rende, CS, Italy. pino72@tiscali.it

ABSTRACT

Background: The alterations of the glutathione peroxidase enzyme complex system occur in physiological conditions such as aging and oxidative stress consequent to strenuous exercise.

Methods: Authors optimize the spectrophotometric method to measure glutathione peroxidase activity in rat red blood cell membranes.

Results: The optimization, when applied to age paired rats, both igravid and pregnant, shows that pregnancy induces, at seventeen d of pregnancy, an increase of both reactive oxygen substance concentration in red blood cells and membrane glutathione peroxidase activity.

Conclusion: The glutathione peroxidase increase in erythrocyte membranes is induced by systemic oxidative stress long lasting rat pregnancy.

Show MeSH
Interaction of GP with the oxidative stress (Merck, Calbiochem 2006/2007 catalogue).
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Figure 2: Interaction of GP with the oxidative stress (Merck, Calbiochem 2006/2007 catalogue).

Mentions: Alterations of this current production of peroxides and free radicals damage structural components of the cell. Reactive oxygen species can be beneficial, as against pathogens, and are also used in cell signalling. Oxidative stress (formulated in Harman's free radical theory of aging) is also thought to contribute to the process. Recent evidence suggests that oxidative stress may also promote life expectancy [3,4]. Recent findings in male support the process and suggest that antioxidants may modulate disease prevalence in humans. The interactions with the oxidative stress are resumed in figure 2.


Red blood cell glutathione peroxidase activity in female igravid and pregnant rats.

Gallo G, Martino G - Reprod. Biol. Endocrinol. (2009)

Interaction of GP with the oxidative stress (Merck, Calbiochem 2006/2007 catalogue).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2657797&req=5

Figure 2: Interaction of GP with the oxidative stress (Merck, Calbiochem 2006/2007 catalogue).
Mentions: Alterations of this current production of peroxides and free radicals damage structural components of the cell. Reactive oxygen species can be beneficial, as against pathogens, and are also used in cell signalling. Oxidative stress (formulated in Harman's free radical theory of aging) is also thought to contribute to the process. Recent evidence suggests that oxidative stress may also promote life expectancy [3,4]. Recent findings in male support the process and suggest that antioxidants may modulate disease prevalence in humans. The interactions with the oxidative stress are resumed in figure 2.

Bottom Line: Authors optimize the spectrophotometric method to measure glutathione peroxidase activity in rat red blood cell membranes.The optimization, when applied to age paired rats, both igravid and pregnant, shows that pregnancy induces, at seventeen d of pregnancy, an increase of both reactive oxygen substance concentration in red blood cells and membrane glutathione peroxidase activity.The glutathione peroxidase increase in erythrocyte membranes is induced by systemic oxidative stress long lasting rat pregnancy.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Cell Biology, University of Calabria, Rende, CS, Italy. pino72@tiscali.it

ABSTRACT

Background: The alterations of the glutathione peroxidase enzyme complex system occur in physiological conditions such as aging and oxidative stress consequent to strenuous exercise.

Methods: Authors optimize the spectrophotometric method to measure glutathione peroxidase activity in rat red blood cell membranes.

Results: The optimization, when applied to age paired rats, both igravid and pregnant, shows that pregnancy induces, at seventeen d of pregnancy, an increase of both reactive oxygen substance concentration in red blood cells and membrane glutathione peroxidase activity.

Conclusion: The glutathione peroxidase increase in erythrocyte membranes is induced by systemic oxidative stress long lasting rat pregnancy.

Show MeSH