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Bovine endometrial stromal cells display osteogenic properties.

Donofrio G, Franceschi V, Capocefalo A, Cavirani S, Sheldon IM - Reprod. Biol. Endocrinol. (2008)

Bottom Line: The endometrium is central to mammalian fertility.In conclusion, the present study has identified that stromal cells from the bovine endometrium show a capability for phenotype plasticity similar to mesenchymal progenitor cells.These observations pave the way for further investigation of the mechanisms of stroma cell differentiation in the bovine reproductive tract.

View Article: PubMed Central - HTML - PubMed

Affiliation: Università di Parma, Facoltà di Medicina Veterinaria, Dipartimento di Salute Animale, Sezione di Malattie Infettive degli Animali, Via del Taglio 8, 43100 Parma, Italy. gaetano.donofrio@unipr.it

ABSTRACT
The endometrium is central to mammalian fertility. The endometrial stromal cells are very dynamic, growing and differentiating throughout the estrous cycle and pregnancy. In humans, stromal cells appear to have progenitor or stem cell capabilities and the cells can even differentiate into bone. It is not clear whether bovine endometrial stromal cells exhibit a similar phenotypic plasticity. So, the present study tested the hypothesis that bovine endometrial stromal cells could be differentiated along an osteogenic lineage. Pure populations of bovine stromal cells were isolated from the endometrium. The endometrial stromal cell phenotype was confirmed by morphology, prostaglandin secretion, and susceptibility to viral infection. However, cultivation of the cells in standard endometrial cell culture medium lead to a mesenchymal phenotype similar to that of bovine bone marrow cells. Furthermore, the endometrial stromal cells developed signs of osteogenesis, such as alizarin positive nodules. When the stromal cells were cultured in a specific osteogenic medium the cells rapidly developed the characteristics of mineralized bone. In conclusion, the present study has identified that stromal cells from the bovine endometrium show a capability for phenotype plasticity similar to mesenchymal progenitor cells. These observations pave the way for further investigation of the mechanisms of stroma cell differentiation in the bovine reproductive tract.

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Representative phase contrast images and fluorescence (green) (10×) of bovine endometrial stromal cells at different time (12, 24, 48, 72 h) post infection (P.I.) with 1 m.o.i. of BoHV-4 EGFPΔTK and the respective phase contrast images of uninfected control. Spreading of the infection can be observed by the green colour invading the field during the time and the CPE is morphologically appreciable by the change of the cell shape, where the cells tend to shrink, becoming roundest and detaching the flask surface. The experiment was repeated three times giving the same result. Each experiment was repeated three times giving similar results.
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Figure 2: Representative phase contrast images and fluorescence (green) (10×) of bovine endometrial stromal cells at different time (12, 24, 48, 72 h) post infection (P.I.) with 1 m.o.i. of BoHV-4 EGFPΔTK and the respective phase contrast images of uninfected control. Spreading of the infection can be observed by the green colour invading the field during the time and the CPE is morphologically appreciable by the change of the cell shape, where the cells tend to shrink, becoming roundest and detaching the flask surface. The experiment was repeated three times giving the same result. Each experiment was repeated three times giving similar results.

Mentions: Endometrial cell cultures were established by the fractional enzyme dissociation method described in Materials and Methods, and there was no mRNA expression of the CD45 pan-leukocyte marker in the epithelial or stromal cells (data not shown) as previously described [15]. The stromal cell purity was estimated to be greater than 95% as determined by microscopy (Fig. 1), preferential secretion of prostaglandin E2 rather than F2α, [12,15] and a high susceptibility to Bovine herpesvirus 4 (Fig. 2) [16].


Bovine endometrial stromal cells display osteogenic properties.

Donofrio G, Franceschi V, Capocefalo A, Cavirani S, Sheldon IM - Reprod. Biol. Endocrinol. (2008)

Representative phase contrast images and fluorescence (green) (10×) of bovine endometrial stromal cells at different time (12, 24, 48, 72 h) post infection (P.I.) with 1 m.o.i. of BoHV-4 EGFPΔTK and the respective phase contrast images of uninfected control. Spreading of the infection can be observed by the green colour invading the field during the time and the CPE is morphologically appreciable by the change of the cell shape, where the cells tend to shrink, becoming roundest and detaching the flask surface. The experiment was repeated three times giving the same result. Each experiment was repeated three times giving similar results.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2657796&req=5

Figure 2: Representative phase contrast images and fluorescence (green) (10×) of bovine endometrial stromal cells at different time (12, 24, 48, 72 h) post infection (P.I.) with 1 m.o.i. of BoHV-4 EGFPΔTK and the respective phase contrast images of uninfected control. Spreading of the infection can be observed by the green colour invading the field during the time and the CPE is morphologically appreciable by the change of the cell shape, where the cells tend to shrink, becoming roundest and detaching the flask surface. The experiment was repeated three times giving the same result. Each experiment was repeated three times giving similar results.
Mentions: Endometrial cell cultures were established by the fractional enzyme dissociation method described in Materials and Methods, and there was no mRNA expression of the CD45 pan-leukocyte marker in the epithelial or stromal cells (data not shown) as previously described [15]. The stromal cell purity was estimated to be greater than 95% as determined by microscopy (Fig. 1), preferential secretion of prostaglandin E2 rather than F2α, [12,15] and a high susceptibility to Bovine herpesvirus 4 (Fig. 2) [16].

Bottom Line: The endometrium is central to mammalian fertility.In conclusion, the present study has identified that stromal cells from the bovine endometrium show a capability for phenotype plasticity similar to mesenchymal progenitor cells.These observations pave the way for further investigation of the mechanisms of stroma cell differentiation in the bovine reproductive tract.

View Article: PubMed Central - HTML - PubMed

Affiliation: Università di Parma, Facoltà di Medicina Veterinaria, Dipartimento di Salute Animale, Sezione di Malattie Infettive degli Animali, Via del Taglio 8, 43100 Parma, Italy. gaetano.donofrio@unipr.it

ABSTRACT
The endometrium is central to mammalian fertility. The endometrial stromal cells are very dynamic, growing and differentiating throughout the estrous cycle and pregnancy. In humans, stromal cells appear to have progenitor or stem cell capabilities and the cells can even differentiate into bone. It is not clear whether bovine endometrial stromal cells exhibit a similar phenotypic plasticity. So, the present study tested the hypothesis that bovine endometrial stromal cells could be differentiated along an osteogenic lineage. Pure populations of bovine stromal cells were isolated from the endometrium. The endometrial stromal cell phenotype was confirmed by morphology, prostaglandin secretion, and susceptibility to viral infection. However, cultivation of the cells in standard endometrial cell culture medium lead to a mesenchymal phenotype similar to that of bovine bone marrow cells. Furthermore, the endometrial stromal cells developed signs of osteogenesis, such as alizarin positive nodules. When the stromal cells were cultured in a specific osteogenic medium the cells rapidly developed the characteristics of mineralized bone. In conclusion, the present study has identified that stromal cells from the bovine endometrium show a capability for phenotype plasticity similar to mesenchymal progenitor cells. These observations pave the way for further investigation of the mechanisms of stroma cell differentiation in the bovine reproductive tract.

Show MeSH
Related in: MedlinePlus