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Kupe virus, a new virus in the family bunyaviridae, genus nairovirus, kenya.

Crabtree MB, Sang R, Miller BR - Emerging Infect. Dis. (2009)

Bottom Line: We have previously described isolation and preliminary identification of a virus related to Dugbe virus (DUGV), family Bunyaviridae, genus Nairovirus.Six isolates of the virus were obtained from pools of Amblyomma gemma and Rhipicephalus pulchellus ticks collected from hides of cattle in Nairobi, Kenya, in October 1999.We suggest that this is a new virus in the family Bunyaviridae, genus Nairovirus, and we propose that it be designated Kupe virus.

View Article: PubMed Central - PubMed

Affiliation: Centers for Disease Control and Prevention, Fort Collins, Colorado, USA. mcrabtree@cdc.gov

ABSTRACT
We have previously described isolation and preliminary identification of a virus related to Dugbe virus (DUGV), family Bunyaviridae, genus Nairovirus. Six isolates of the virus were obtained from pools of Amblyomma gemma and Rhipicephalus pulchellus ticks collected from hides of cattle in Nairobi, Kenya, in October 1999. We report results of further characterization of this virus, including growth kinetics in cell culture and full-length genome sequencing and genetic characterization, which show it to be distinct from DUGV. We suggest that this is a new virus in the family Bunyaviridae, genus Nairovirus, and we propose that it be designated Kupe virus.

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Phylogenetic tree produced by using maximum parsimony analysis with 500 bootstrap replicates on amino acid alignment of nairovirus large segment fragment (147 aa sequence translated from 441 nt sequence). Scale bar indicates branch length and bootstrap values >50% are shown above branches.
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Figure 3: Phylogenetic tree produced by using maximum parsimony analysis with 500 bootstrap replicates on amino acid alignment of nairovirus large segment fragment (147 aa sequence translated from 441 nt sequence). Scale bar indicates branch length and bootstrap values >50% are shown above branches.

Mentions: Nucleotide and amino acid sequence comparisons of a 441-nt fragment of the highly conserved L segment RDRP catalytic core domain (Kupe virus nt 6986–7426) were also made between Kupe virus and sequences of 14 other viruses representing 7 groups of the Nairovirus genus published by Honig et al. (19). A phylogenetic tree derived from the amino acid alignment of these sequences shows Kupe virus most closely related to DUGV (82.8% nt identity/95.9% aa identity), NSDV (74.9%/92.5%), CCHFV (71.9%/88.4%), and HAZV (71.7%/87.8%) (Figure 3). An additional 603-nt L fragment alignment overlapping the RDRP core domain (Kupe virus nt 7292–7894) included sequences from 26 DUGV isolates obtained in Kenya in 1999 (GenBank accession nos. EU359010–EU359035), DUGV ArD 44313, and Kupe virus. Results of these comparisons are shown in Table 6. Sequence identities among 5 Kupe virus isolates for this fragment were nt 91.2%–100.0% and aa 98.5%–100.0% (GenBank accession nos. EU257628, EU816898–EU816901).


Kupe virus, a new virus in the family bunyaviridae, genus nairovirus, kenya.

Crabtree MB, Sang R, Miller BR - Emerging Infect. Dis. (2009)

Phylogenetic tree produced by using maximum parsimony analysis with 500 bootstrap replicates on amino acid alignment of nairovirus large segment fragment (147 aa sequence translated from 441 nt sequence). Scale bar indicates branch length and bootstrap values >50% are shown above branches.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2657624&req=5

Figure 3: Phylogenetic tree produced by using maximum parsimony analysis with 500 bootstrap replicates on amino acid alignment of nairovirus large segment fragment (147 aa sequence translated from 441 nt sequence). Scale bar indicates branch length and bootstrap values >50% are shown above branches.
Mentions: Nucleotide and amino acid sequence comparisons of a 441-nt fragment of the highly conserved L segment RDRP catalytic core domain (Kupe virus nt 6986–7426) were also made between Kupe virus and sequences of 14 other viruses representing 7 groups of the Nairovirus genus published by Honig et al. (19). A phylogenetic tree derived from the amino acid alignment of these sequences shows Kupe virus most closely related to DUGV (82.8% nt identity/95.9% aa identity), NSDV (74.9%/92.5%), CCHFV (71.9%/88.4%), and HAZV (71.7%/87.8%) (Figure 3). An additional 603-nt L fragment alignment overlapping the RDRP core domain (Kupe virus nt 7292–7894) included sequences from 26 DUGV isolates obtained in Kenya in 1999 (GenBank accession nos. EU359010–EU359035), DUGV ArD 44313, and Kupe virus. Results of these comparisons are shown in Table 6. Sequence identities among 5 Kupe virus isolates for this fragment were nt 91.2%–100.0% and aa 98.5%–100.0% (GenBank accession nos. EU257628, EU816898–EU816901).

Bottom Line: We have previously described isolation and preliminary identification of a virus related to Dugbe virus (DUGV), family Bunyaviridae, genus Nairovirus.Six isolates of the virus were obtained from pools of Amblyomma gemma and Rhipicephalus pulchellus ticks collected from hides of cattle in Nairobi, Kenya, in October 1999.We suggest that this is a new virus in the family Bunyaviridae, genus Nairovirus, and we propose that it be designated Kupe virus.

View Article: PubMed Central - PubMed

Affiliation: Centers for Disease Control and Prevention, Fort Collins, Colorado, USA. mcrabtree@cdc.gov

ABSTRACT
We have previously described isolation and preliminary identification of a virus related to Dugbe virus (DUGV), family Bunyaviridae, genus Nairovirus. Six isolates of the virus were obtained from pools of Amblyomma gemma and Rhipicephalus pulchellus ticks collected from hides of cattle in Nairobi, Kenya, in October 1999. We report results of further characterization of this virus, including growth kinetics in cell culture and full-length genome sequencing and genetic characterization, which show it to be distinct from DUGV. We suggest that this is a new virus in the family Bunyaviridae, genus Nairovirus, and we propose that it be designated Kupe virus.

Show MeSH
Related in: MedlinePlus