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Tahyna virus and human infection, China.

Lu Z, Lu XJ, Fu SH, Zhang S, Li ZX, Yao XH, Feng YP, Lambert AJ, Ni da X, Wang FT, Tong SX, Nasci RS, Feng Y, Dong Q, Zhai YG, Gao XY, Wang HY, Tang Q, Liang GD - Emerging Infect. Dis. (2009)

Bottom Line: In 2006, Tahyna virus was isolated from Culex spp. mosquitoes collected in Xinjiang, People's Republic of China.In 2007, to determine whether this virus was infecting humans, we tested serum from febrile patients.We found immunoglobulin (Ig) M and IgG against the virus, which suggests human infection in this region.

View Article: PubMed Central - PubMed

Affiliation: Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China.

ABSTRACT
In 2006, Tahyna virus was isolated from Culex spp. mosquitoes collected in Xinjiang, People's Republic of China. In 2007, to determine whether this virus was infecting humans, we tested serum from febrile patients. We found immunoglobulin (Ig) M and IgG against the virus, which suggests human infection in this region.

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Related in: MedlinePlus

Phylogenetic analysis of Tahyna virus (TAHV) XJ0625 from China based on the complete nucleotide sequence of the small segment (A) and the medium segment (B). Distances and groupings were determined by the p-distance algorithm and neighbor-joining method with MEGA version 3.1 software (www.megasoftware.net). Bootstrap values are indicated and correspond to 1,000 replications. The tree was rooted by using Bunyamwera virus as the outgroup virus. Scale bars indicate a genetic distance of 0.05-nt substitutions per position.
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Figure 2: Phylogenetic analysis of Tahyna virus (TAHV) XJ0625 from China based on the complete nucleotide sequence of the small segment (A) and the medium segment (B). Distances and groupings were determined by the p-distance algorithm and neighbor-joining method with MEGA version 3.1 software (www.megasoftware.net). Bootstrap values are indicated and correspond to 1,000 replications. The tree was rooted by using Bunyamwera virus as the outgroup virus. Scale bars indicate a genetic distance of 0.05-nt substitutions per position.

Mentions: Phylogenetic analyses of the nucleotide sequences of the S (Figure 2, panel A) and M (Figure 2, panel B) segments generated highly comparable topologies, which indicates that XJ0625 has a high level of sequence homology with TAHV. To provide independent confirmation, we sent the XJ0625 viral RNA to CDC, Fort Collins, Colorado, USA, for further characterization. RNA was subjected to RT-PCR amplification by using multiple primer sets designed for the detection of orthobunyavirus S segment RNA (11). Nucleotide sequencing of amplified DNA fragments that, in combination, span the entirety of the TAHV S segment was used to positively identify XJ0625 viral RNA as TAHV RNA (data not shown) (15).


Tahyna virus and human infection, China.

Lu Z, Lu XJ, Fu SH, Zhang S, Li ZX, Yao XH, Feng YP, Lambert AJ, Ni da X, Wang FT, Tong SX, Nasci RS, Feng Y, Dong Q, Zhai YG, Gao XY, Wang HY, Tang Q, Liang GD - Emerging Infect. Dis. (2009)

Phylogenetic analysis of Tahyna virus (TAHV) XJ0625 from China based on the complete nucleotide sequence of the small segment (A) and the medium segment (B). Distances and groupings were determined by the p-distance algorithm and neighbor-joining method with MEGA version 3.1 software (www.megasoftware.net). Bootstrap values are indicated and correspond to 1,000 replications. The tree was rooted by using Bunyamwera virus as the outgroup virus. Scale bars indicate a genetic distance of 0.05-nt substitutions per position.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2657618&req=5

Figure 2: Phylogenetic analysis of Tahyna virus (TAHV) XJ0625 from China based on the complete nucleotide sequence of the small segment (A) and the medium segment (B). Distances and groupings were determined by the p-distance algorithm and neighbor-joining method with MEGA version 3.1 software (www.megasoftware.net). Bootstrap values are indicated and correspond to 1,000 replications. The tree was rooted by using Bunyamwera virus as the outgroup virus. Scale bars indicate a genetic distance of 0.05-nt substitutions per position.
Mentions: Phylogenetic analyses of the nucleotide sequences of the S (Figure 2, panel A) and M (Figure 2, panel B) segments generated highly comparable topologies, which indicates that XJ0625 has a high level of sequence homology with TAHV. To provide independent confirmation, we sent the XJ0625 viral RNA to CDC, Fort Collins, Colorado, USA, for further characterization. RNA was subjected to RT-PCR amplification by using multiple primer sets designed for the detection of orthobunyavirus S segment RNA (11). Nucleotide sequencing of amplified DNA fragments that, in combination, span the entirety of the TAHV S segment was used to positively identify XJ0625 viral RNA as TAHV RNA (data not shown) (15).

Bottom Line: In 2006, Tahyna virus was isolated from Culex spp. mosquitoes collected in Xinjiang, People's Republic of China.In 2007, to determine whether this virus was infecting humans, we tested serum from febrile patients.We found immunoglobulin (Ig) M and IgG against the virus, which suggests human infection in this region.

View Article: PubMed Central - PubMed

Affiliation: Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China.

ABSTRACT
In 2006, Tahyna virus was isolated from Culex spp. mosquitoes collected in Xinjiang, People's Republic of China. In 2007, to determine whether this virus was infecting humans, we tested serum from febrile patients. We found immunoglobulin (Ig) M and IgG against the virus, which suggests human infection in this region.

Show MeSH
Related in: MedlinePlus