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Salicylic acid deficiency in NahG transgenic lines and sid2 mutants increases seed yield in the annual plant Arabidopsis thaliana.

Abreu ME, Munné-Bosch S - J. Exp. Bot. (2009)

Bottom Line: Salicylic acid-deficient NahG transgenic lines and sid2 mutants were used to evaluate the role of this compound in the development of the short-lived, annual plant Arabidopsis thaliana, with a particular focus on the interplay between salicylic acid and other phytohormones.Low salicylic acid levels led to increased growth, as well as to smaller abscisic acid levels and reduced damage to PSII (as indicated by F(v)/F(m) ratios) during the reproductive stages in rosette leaves of NahG transgenic lines and sid2 mutants, compared with wild-type plants.It is concluded that (i) the sid2 gene, which encodes for isochorismate synthase, plays a central role in salicylic acid biosynthesis during plant development in A. thaliana, (ii) salicylic acid plays a role in the regulation of growth, senescence, and seed production, (iii) there is a cross-talk between salicylic acid and other phytohormones during plant development, and (iv) the concentrations of antioxidant vitamins in seeds may be influenced by the endogenous levels of salicylic acid in plants.

View Article: PubMed Central - PubMed

Affiliation: Departament de Biologia Vegetal, Universitat de Barcelona, Facultat de Biologia, Avinguda Diagonal 645, E-08028 Barcelona, Spain.

ABSTRACT
Salicylic acid-deficient NahG transgenic lines and sid2 mutants were used to evaluate the role of this compound in the development of the short-lived, annual plant Arabidopsis thaliana, with a particular focus on the interplay between salicylic acid and other phytohormones. Low salicylic acid levels led to increased growth, as well as to smaller abscisic acid levels and reduced damage to PSII (as indicated by F(v)/F(m) ratios) during the reproductive stages in rosette leaves of NahG transgenic lines and sid2 mutants, compared with wild-type plants. Furthermore, salicylic acid deficiency highly influenced seed yield and composition. Seed production increased by 4.4-fold and 3.5-fold in NahG transgenic lines and sid2 mutants, respectively, compared to the wild type. Salicylic acid deficiency also improved seed composition in terms of antioxidant vitamin concentrations, seeds of salicylic acid-deficient plants showing higher levels of alpha- and gamma-tocopherol (vitamin E) and beta-carotene (pro-vitamin A) than seeds of wild-type plants. Seeds of salicylic acid-deficient plants also showed higher nitrogen concentrations than seeds of wild-type plants. It is concluded that (i) the sid2 gene, which encodes for isochorismate synthase, plays a central role in salicylic acid biosynthesis during plant development in A. thaliana, (ii) salicylic acid plays a role in the regulation of growth, senescence, and seed production, (iii) there is a cross-talk between salicylic acid and other phytohormones during plant development, and (iv) the concentrations of antioxidant vitamins in seeds may be influenced by the endogenous levels of salicylic acid in plants.

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Endogenous concentrations of indole-3-acetic acid (IAA), jasmonic acid (JA), abscisic acid (ABA), gibberellin 4 (GA4), zeatin (Z), and zeatin riboside (ZR) in seeds of wild type and SA-deficient NahG transgenic lines and sid2 mutants of A. thaliana. Data represent the mean ±SE of four measurements. Letters indicate statistical significant differences between seeds of different plant groups at a probability level of P ≤0.05.
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fig5: Endogenous concentrations of indole-3-acetic acid (IAA), jasmonic acid (JA), abscisic acid (ABA), gibberellin 4 (GA4), zeatin (Z), and zeatin riboside (ZR) in seeds of wild type and SA-deficient NahG transgenic lines and sid2 mutants of A. thaliana. Data represent the mean ±SE of four measurements. Letters indicate statistical significant differences between seeds of different plant groups at a probability level of P ≤0.05.

Mentions: The analysis of hormonal levels in seeds did not reveal significant differences between wild-type plants and NahG transgenic lines, whose seeds at maturity contained SA levels of ca. 310 ng and 40 ng [g dry wt]−1, respectively. Seeds of sid2 mutants, which contained SA levels of ca. 0.2 ng [g dry wt]−1 showed, however, significant alterations in the hormonal balance. The levels of IAA in seeds of the sid2 mutants increased 5-fold compared with those of the wild type and NahG transgenic lines, while the levels of the cytokinins, zeatin, and zeatin riboside were reduced by ca. 50% in seeds of this mutant compared to wild type (Fig. 5).


Salicylic acid deficiency in NahG transgenic lines and sid2 mutants increases seed yield in the annual plant Arabidopsis thaliana.

Abreu ME, Munné-Bosch S - J. Exp. Bot. (2009)

Endogenous concentrations of indole-3-acetic acid (IAA), jasmonic acid (JA), abscisic acid (ABA), gibberellin 4 (GA4), zeatin (Z), and zeatin riboside (ZR) in seeds of wild type and SA-deficient NahG transgenic lines and sid2 mutants of A. thaliana. Data represent the mean ±SE of four measurements. Letters indicate statistical significant differences between seeds of different plant groups at a probability level of P ≤0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2657544&req=5

fig5: Endogenous concentrations of indole-3-acetic acid (IAA), jasmonic acid (JA), abscisic acid (ABA), gibberellin 4 (GA4), zeatin (Z), and zeatin riboside (ZR) in seeds of wild type and SA-deficient NahG transgenic lines and sid2 mutants of A. thaliana. Data represent the mean ±SE of four measurements. Letters indicate statistical significant differences between seeds of different plant groups at a probability level of P ≤0.05.
Mentions: The analysis of hormonal levels in seeds did not reveal significant differences between wild-type plants and NahG transgenic lines, whose seeds at maturity contained SA levels of ca. 310 ng and 40 ng [g dry wt]−1, respectively. Seeds of sid2 mutants, which contained SA levels of ca. 0.2 ng [g dry wt]−1 showed, however, significant alterations in the hormonal balance. The levels of IAA in seeds of the sid2 mutants increased 5-fold compared with those of the wild type and NahG transgenic lines, while the levels of the cytokinins, zeatin, and zeatin riboside were reduced by ca. 50% in seeds of this mutant compared to wild type (Fig. 5).

Bottom Line: Salicylic acid-deficient NahG transgenic lines and sid2 mutants were used to evaluate the role of this compound in the development of the short-lived, annual plant Arabidopsis thaliana, with a particular focus on the interplay between salicylic acid and other phytohormones.Low salicylic acid levels led to increased growth, as well as to smaller abscisic acid levels and reduced damage to PSII (as indicated by F(v)/F(m) ratios) during the reproductive stages in rosette leaves of NahG transgenic lines and sid2 mutants, compared with wild-type plants.It is concluded that (i) the sid2 gene, which encodes for isochorismate synthase, plays a central role in salicylic acid biosynthesis during plant development in A. thaliana, (ii) salicylic acid plays a role in the regulation of growth, senescence, and seed production, (iii) there is a cross-talk between salicylic acid and other phytohormones during plant development, and (iv) the concentrations of antioxidant vitamins in seeds may be influenced by the endogenous levels of salicylic acid in plants.

View Article: PubMed Central - PubMed

Affiliation: Departament de Biologia Vegetal, Universitat de Barcelona, Facultat de Biologia, Avinguda Diagonal 645, E-08028 Barcelona, Spain.

ABSTRACT
Salicylic acid-deficient NahG transgenic lines and sid2 mutants were used to evaluate the role of this compound in the development of the short-lived, annual plant Arabidopsis thaliana, with a particular focus on the interplay between salicylic acid and other phytohormones. Low salicylic acid levels led to increased growth, as well as to smaller abscisic acid levels and reduced damage to PSII (as indicated by F(v)/F(m) ratios) during the reproductive stages in rosette leaves of NahG transgenic lines and sid2 mutants, compared with wild-type plants. Furthermore, salicylic acid deficiency highly influenced seed yield and composition. Seed production increased by 4.4-fold and 3.5-fold in NahG transgenic lines and sid2 mutants, respectively, compared to the wild type. Salicylic acid deficiency also improved seed composition in terms of antioxidant vitamin concentrations, seeds of salicylic acid-deficient plants showing higher levels of alpha- and gamma-tocopherol (vitamin E) and beta-carotene (pro-vitamin A) than seeds of wild-type plants. Seeds of salicylic acid-deficient plants also showed higher nitrogen concentrations than seeds of wild-type plants. It is concluded that (i) the sid2 gene, which encodes for isochorismate synthase, plays a central role in salicylic acid biosynthesis during plant development in A. thaliana, (ii) salicylic acid plays a role in the regulation of growth, senescence, and seed production, (iii) there is a cross-talk between salicylic acid and other phytohormones during plant development, and (iv) the concentrations of antioxidant vitamins in seeds may be influenced by the endogenous levels of salicylic acid in plants.

Show MeSH
Related in: MedlinePlus