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Expression pattern of four storage xyloglucan mobilization-related genes during seedling development of the rain forest tree Hymenaea courbaril L.

Brandão AD, Del Bem LE, Vincentz M, Buckeridge MS - J. Exp. Bot. (2009)

Bottom Line: The partial sequences were characterized by comparison with sequences available in the literature, and phylogenetic trees were assembled.HcXTH1 and HcBGAL1 were correlated to xyloglucan degradation and responded to auxin and light, being down-regulated when transport of auxin was prevented by N-1-naphthylphthalamic acid (NPA) and stimulated by constant light.It is concluded that gene expression plays a role in the control of the intercommunication system of the source-sink relationship during seeding growth, favouring its establishment in the shaded environment of the rain forest understorey.

View Article: PubMed Central - PubMed

Affiliation: Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.

ABSTRACT
During seedling establishment, cotyledons of the rain forest tree Hymenaea courbaril mobilize storage cell wall xyloglucan to sustain growth. The polysaccharide is degraded and its products are transported to growing sink tissues. Auxin from the shoot controls the level of xyloglucan hydrolytic enzymes. It is not yet known how important the expression of these genes is for the control of storage xyloglucan degradation. In this work, partial cDNAs of the genes xyloglucan transglycosylase hydrolase (HcXTH1) and beta-galactosidase (HcBGAL1), both related to xyloglucan degradation, and two other genes related to sucrose metabolism [alkaline invertase (HcAlkIN1) and sucrose synthase (HcSUS1)], were isolated. The partial sequences were characterized by comparison with sequences available in the literature, and phylogenetic trees were assembled. Gene expression was evaluated at intervals of 6 h during 24 h in cotyledons, hypocotyl, roots, and leaves, using 45-d-old plantlets. HcXTH1 and HcBGAL1 were correlated to xyloglucan degradation and responded to auxin and light, being down-regulated when transport of auxin was prevented by N-1-naphthylphthalamic acid (NPA) and stimulated by constant light. Genes related to sucrose metabolism, HcAlkIN1 and HcSUS1, responded to inhibition of auxin transport in consonance with storage mobilization in the cotyledons. A model is proposed suggesting that auxin and light are involved in the control of the expression of genes related to storage xyloglucan mobilization in seedlings of H. courbaril. It is concluded that gene expression plays a role in the control of the intercommunication system of the source-sink relationship during seeding growth, favouring its establishment in the shaded environment of the rain forest understorey.

Show MeSH
Phylogenetic relationship of alkaline/neutral invertase of Angiosperms. Distances were calculated on the basis of positions 278–556 of A. thaliana (AT4G34860). The nucleotide sequence can be accessed at NCBI: EU370968. Accession numbers are as follows: A. thaliana (nine members), Oryza sativa (OsNIN1, AY575558; OsNIN2, AY575559; OsNIN3, AY575560; OsNIN4, AY575561; OsNIN5, AY575562; OsNIN6, AY575563; OsNIN7, AY575564; OsNIN8, AY575565), and Lotus corniculatus (Lc-inv1, AJ717412). The values presented represent the bootstraps.
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fig4: Phylogenetic relationship of alkaline/neutral invertase of Angiosperms. Distances were calculated on the basis of positions 278–556 of A. thaliana (AT4G34860). The nucleotide sequence can be accessed at NCBI: EU370968. Accession numbers are as follows: A. thaliana (nine members), Oryza sativa (OsNIN1, AY575558; OsNIN2, AY575559; OsNIN3, AY575560; OsNIN4, AY575561; OsNIN5, AY575562; OsNIN6, AY575563; OsNIN7, AY575564; OsNIN8, AY575565), and Lotus corniculatus (Lc-inv1, AJ717412). The values presented represent the bootstraps.

Mentions: Relatively little is known about the features of genes related to alkaline invertases. HcAlkIN1 was found to be close to a gene from the legume Lotus corniculatus (Lcinv1) (Fig. 4). This class of genes is referred to as neutral alkaline invertase in the literature and its function is poorly understood.


Expression pattern of four storage xyloglucan mobilization-related genes during seedling development of the rain forest tree Hymenaea courbaril L.

Brandão AD, Del Bem LE, Vincentz M, Buckeridge MS - J. Exp. Bot. (2009)

Phylogenetic relationship of alkaline/neutral invertase of Angiosperms. Distances were calculated on the basis of positions 278–556 of A. thaliana (AT4G34860). The nucleotide sequence can be accessed at NCBI: EU370968. Accession numbers are as follows: A. thaliana (nine members), Oryza sativa (OsNIN1, AY575558; OsNIN2, AY575559; OsNIN3, AY575560; OsNIN4, AY575561; OsNIN5, AY575562; OsNIN6, AY575563; OsNIN7, AY575564; OsNIN8, AY575565), and Lotus corniculatus (Lc-inv1, AJ717412). The values presented represent the bootstraps.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2657543&req=5

fig4: Phylogenetic relationship of alkaline/neutral invertase of Angiosperms. Distances were calculated on the basis of positions 278–556 of A. thaliana (AT4G34860). The nucleotide sequence can be accessed at NCBI: EU370968. Accession numbers are as follows: A. thaliana (nine members), Oryza sativa (OsNIN1, AY575558; OsNIN2, AY575559; OsNIN3, AY575560; OsNIN4, AY575561; OsNIN5, AY575562; OsNIN6, AY575563; OsNIN7, AY575564; OsNIN8, AY575565), and Lotus corniculatus (Lc-inv1, AJ717412). The values presented represent the bootstraps.
Mentions: Relatively little is known about the features of genes related to alkaline invertases. HcAlkIN1 was found to be close to a gene from the legume Lotus corniculatus (Lcinv1) (Fig. 4). This class of genes is referred to as neutral alkaline invertase in the literature and its function is poorly understood.

Bottom Line: The partial sequences were characterized by comparison with sequences available in the literature, and phylogenetic trees were assembled.HcXTH1 and HcBGAL1 were correlated to xyloglucan degradation and responded to auxin and light, being down-regulated when transport of auxin was prevented by N-1-naphthylphthalamic acid (NPA) and stimulated by constant light.It is concluded that gene expression plays a role in the control of the intercommunication system of the source-sink relationship during seeding growth, favouring its establishment in the shaded environment of the rain forest understorey.

View Article: PubMed Central - PubMed

Affiliation: Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.

ABSTRACT
During seedling establishment, cotyledons of the rain forest tree Hymenaea courbaril mobilize storage cell wall xyloglucan to sustain growth. The polysaccharide is degraded and its products are transported to growing sink tissues. Auxin from the shoot controls the level of xyloglucan hydrolytic enzymes. It is not yet known how important the expression of these genes is for the control of storage xyloglucan degradation. In this work, partial cDNAs of the genes xyloglucan transglycosylase hydrolase (HcXTH1) and beta-galactosidase (HcBGAL1), both related to xyloglucan degradation, and two other genes related to sucrose metabolism [alkaline invertase (HcAlkIN1) and sucrose synthase (HcSUS1)], were isolated. The partial sequences were characterized by comparison with sequences available in the literature, and phylogenetic trees were assembled. Gene expression was evaluated at intervals of 6 h during 24 h in cotyledons, hypocotyl, roots, and leaves, using 45-d-old plantlets. HcXTH1 and HcBGAL1 were correlated to xyloglucan degradation and responded to auxin and light, being down-regulated when transport of auxin was prevented by N-1-naphthylphthalamic acid (NPA) and stimulated by constant light. Genes related to sucrose metabolism, HcAlkIN1 and HcSUS1, responded to inhibition of auxin transport in consonance with storage mobilization in the cotyledons. A model is proposed suggesting that auxin and light are involved in the control of the expression of genes related to storage xyloglucan mobilization in seedlings of H. courbaril. It is concluded that gene expression plays a role in the control of the intercommunication system of the source-sink relationship during seeding growth, favouring its establishment in the shaded environment of the rain forest understorey.

Show MeSH