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Attacks by a piercing-sucking insect (Myzus persicae Sultzer) or a chewing insect (Leptinotarsa decemlineata Say) on potato plants (Solanum tuberosum L.) induce differential changes in volatile compound release and oxylipin synthesis.

Gosset V, Harmel N, Göbel C, Francis F, Haubruge E, Wathelet JP, du Jardin P, Feussner I, Fauconnier ML - J. Exp. Bot. (2009)

Bottom Line: Hydroperoxides are also precursors of JA and some volatile compounds.In addition, 9-LOX products, which are usually associated with defence against pathogens, were exclusively activated by aphid attack.Furthermore, a correlation between volatiles and JA accumulation and the evolution of their precursors was determined.

View Article: PubMed Central - PubMed

Affiliation: Plant Biology Unit, Gembloux Agricultural University, Passage des déportés 2, B-5030 Gembloux, Belgium.

ABSTRACT
Plant defensive strategies bring into play blends of compounds dependent on the type of attacker and coming from different synthesis pathways. Interest in the field is mainly focused on volatile organic compounds (VOCs) and jasmonic acid (JA). By contrast, little is known about the oxidized polyunsaturated fatty acids (PUFAs), such as PUFA-hydroperoxides, PUFA-hydroxides, or PUFA-ketones. PUFA-hydroperoxides and their derivatives might be involved in stress response and show antimicrobial activities. Hydroperoxides are also precursors of JA and some volatile compounds. In this paper, the differential biochemical response of a plant against insects with distinct feeding behaviours is characterized not only in terms of VOC signature and JA profile but also in terms of their precursors synthesized through the lipoxygenase (LOX)-pathway at the early stage of the plant response. For this purpose, two leading pests of potato with distinct feeding behaviours were used: the Colorado Potato Beetle (Leptinotarsa decemlineata Say), a chewing herbivore, and the Green Peach Aphid (Myzus persicae Sulzer), a piercing-sucking insect. The volatile signatures identified clearly differ in function with the feeding behaviour of the attacker and the aphid, which causes the smaller damages, triggers the emission of a higher number of volatiles. In addition, 9-LOX products, which are usually associated with defence against pathogens, were exclusively activated by aphid attack. Furthermore, a correlation between volatiles and JA accumulation and the evolution of their precursors was determined. Finally, the role of the insect itself on the plant response after insect infestation was highlighted.

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(A) Ratio between 9-HPOD content 8 h, 24 h, 48 h, and 72 h in aphid-infested or mechanically pricked plants and in control plants. (B) Ratio between 13-HPOT content 8 h, 24 h, 48 h, and 72 h in CPB-infested or mechanically perforated plants and in control plants. (C) Ratio between 13-HPOT content 8 h, 24 h, 48 h, and 72 h in aphid-infested or mechanically pricked plants and in control plants. The data are the average of two repetitions and are expressed as a ratio between the oxylipin concentration 8 h, 24 h, 48 h or 72 h after the beginning of the experiment and the oxylipin concentration in control plants (0 h). In all figures, the values correspond to the mean and the error bars to the standard deviations. After one-way variance analysis means were classified using Student's t test. Differences between means were considered to be significantly different at P <0.05.
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fig6: (A) Ratio between 9-HPOD content 8 h, 24 h, 48 h, and 72 h in aphid-infested or mechanically pricked plants and in control plants. (B) Ratio between 13-HPOT content 8 h, 24 h, 48 h, and 72 h in CPB-infested or mechanically perforated plants and in control plants. (C) Ratio between 13-HPOT content 8 h, 24 h, 48 h, and 72 h in aphid-infested or mechanically pricked plants and in control plants. The data are the average of two repetitions and are expressed as a ratio between the oxylipin concentration 8 h, 24 h, 48 h or 72 h after the beginning of the experiment and the oxylipin concentration in control plants (0 h). In all figures, the values correspond to the mean and the error bars to the standard deviations. After one-way variance analysis means were classified using Student's t test. Differences between means were considered to be significantly different at P <0.05.

Mentions: 9-HPOD and 13-HPOT synthesis were activated after aphid attack and CPB attack, respectively (Fig. 5). The same compounds were activated after the corresponding mechanical wounding but with different kinetics (Fig. 6). Indeed, whereas the activation of 13-HPOT synthesis was observed 48 h and 72 h after CPB infestation and the activation of 9-HPOD synthesis was observed 48 h and 72 h after aphid infestation (Fig. 5), a significant increase in 9-HPOD was observed 72 h after needle pricks (Fig. 6A) and a significant increase in 13-HPOT content was observed 8 h, 24 h, 48 h, and 72 h after perforations (Fig. 6B). Therefore, the activation of 9-HPOD synthesis is faster against aphids than against needle pricks but 9-HPOD content 48 h (0.362±0.012 nmol g−1 FW) and 72 h (0.366±0.079 nmol g−1 FW) after aphid attack as well as 9-HPOD content 72 h (0.439±0.065 nmol g−1 FW) after needle pricks are statistically similar. On the other hand, 13-HPOT activation is faster after perforations than after CPB-attacks and the maximum content in 13-HPOT after perforations (0.781±0.033 nmol g−1 FW) is significantly higher than the maximum content in 13-HPOT after CPB infestation (0.552±0.022 nmol g−1 FW) (P = 0.015). Therefore, activation of 13-HPOT synthesis is faster and stronger after perforations than after CPB-attacks.


Attacks by a piercing-sucking insect (Myzus persicae Sultzer) or a chewing insect (Leptinotarsa decemlineata Say) on potato plants (Solanum tuberosum L.) induce differential changes in volatile compound release and oxylipin synthesis.

Gosset V, Harmel N, Göbel C, Francis F, Haubruge E, Wathelet JP, du Jardin P, Feussner I, Fauconnier ML - J. Exp. Bot. (2009)

(A) Ratio between 9-HPOD content 8 h, 24 h, 48 h, and 72 h in aphid-infested or mechanically pricked plants and in control plants. (B) Ratio between 13-HPOT content 8 h, 24 h, 48 h, and 72 h in CPB-infested or mechanically perforated plants and in control plants. (C) Ratio between 13-HPOT content 8 h, 24 h, 48 h, and 72 h in aphid-infested or mechanically pricked plants and in control plants. The data are the average of two repetitions and are expressed as a ratio between the oxylipin concentration 8 h, 24 h, 48 h or 72 h after the beginning of the experiment and the oxylipin concentration in control plants (0 h). In all figures, the values correspond to the mean and the error bars to the standard deviations. After one-way variance analysis means were classified using Student's t test. Differences between means were considered to be significantly different at P <0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2657539&req=5

fig6: (A) Ratio between 9-HPOD content 8 h, 24 h, 48 h, and 72 h in aphid-infested or mechanically pricked plants and in control plants. (B) Ratio between 13-HPOT content 8 h, 24 h, 48 h, and 72 h in CPB-infested or mechanically perforated plants and in control plants. (C) Ratio between 13-HPOT content 8 h, 24 h, 48 h, and 72 h in aphid-infested or mechanically pricked plants and in control plants. The data are the average of two repetitions and are expressed as a ratio between the oxylipin concentration 8 h, 24 h, 48 h or 72 h after the beginning of the experiment and the oxylipin concentration in control plants (0 h). In all figures, the values correspond to the mean and the error bars to the standard deviations. After one-way variance analysis means were classified using Student's t test. Differences between means were considered to be significantly different at P <0.05.
Mentions: 9-HPOD and 13-HPOT synthesis were activated after aphid attack and CPB attack, respectively (Fig. 5). The same compounds were activated after the corresponding mechanical wounding but with different kinetics (Fig. 6). Indeed, whereas the activation of 13-HPOT synthesis was observed 48 h and 72 h after CPB infestation and the activation of 9-HPOD synthesis was observed 48 h and 72 h after aphid infestation (Fig. 5), a significant increase in 9-HPOD was observed 72 h after needle pricks (Fig. 6A) and a significant increase in 13-HPOT content was observed 8 h, 24 h, 48 h, and 72 h after perforations (Fig. 6B). Therefore, the activation of 9-HPOD synthesis is faster against aphids than against needle pricks but 9-HPOD content 48 h (0.362±0.012 nmol g−1 FW) and 72 h (0.366±0.079 nmol g−1 FW) after aphid attack as well as 9-HPOD content 72 h (0.439±0.065 nmol g−1 FW) after needle pricks are statistically similar. On the other hand, 13-HPOT activation is faster after perforations than after CPB-attacks and the maximum content in 13-HPOT after perforations (0.781±0.033 nmol g−1 FW) is significantly higher than the maximum content in 13-HPOT after CPB infestation (0.552±0.022 nmol g−1 FW) (P = 0.015). Therefore, activation of 13-HPOT synthesis is faster and stronger after perforations than after CPB-attacks.

Bottom Line: Hydroperoxides are also precursors of JA and some volatile compounds.In addition, 9-LOX products, which are usually associated with defence against pathogens, were exclusively activated by aphid attack.Furthermore, a correlation between volatiles and JA accumulation and the evolution of their precursors was determined.

View Article: PubMed Central - PubMed

Affiliation: Plant Biology Unit, Gembloux Agricultural University, Passage des déportés 2, B-5030 Gembloux, Belgium.

ABSTRACT
Plant defensive strategies bring into play blends of compounds dependent on the type of attacker and coming from different synthesis pathways. Interest in the field is mainly focused on volatile organic compounds (VOCs) and jasmonic acid (JA). By contrast, little is known about the oxidized polyunsaturated fatty acids (PUFAs), such as PUFA-hydroperoxides, PUFA-hydroxides, or PUFA-ketones. PUFA-hydroperoxides and their derivatives might be involved in stress response and show antimicrobial activities. Hydroperoxides are also precursors of JA and some volatile compounds. In this paper, the differential biochemical response of a plant against insects with distinct feeding behaviours is characterized not only in terms of VOC signature and JA profile but also in terms of their precursors synthesized through the lipoxygenase (LOX)-pathway at the early stage of the plant response. For this purpose, two leading pests of potato with distinct feeding behaviours were used: the Colorado Potato Beetle (Leptinotarsa decemlineata Say), a chewing herbivore, and the Green Peach Aphid (Myzus persicae Sulzer), a piercing-sucking insect. The volatile signatures identified clearly differ in function with the feeding behaviour of the attacker and the aphid, which causes the smaller damages, triggers the emission of a higher number of volatiles. In addition, 9-LOX products, which are usually associated with defence against pathogens, were exclusively activated by aphid attack. Furthermore, a correlation between volatiles and JA accumulation and the evolution of their precursors was determined. Finally, the role of the insect itself on the plant response after insect infestation was highlighted.

Show MeSH
Related in: MedlinePlus