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Attacks by a piercing-sucking insect (Myzus persicae Sultzer) or a chewing insect (Leptinotarsa decemlineata Say) on potato plants (Solanum tuberosum L.) induce differential changes in volatile compound release and oxylipin synthesis.

Gosset V, Harmel N, Göbel C, Francis F, Haubruge E, Wathelet JP, du Jardin P, Feussner I, Fauconnier ML - J. Exp. Bot. (2009)

Bottom Line: Hydroperoxides are also precursors of JA and some volatile compounds.In addition, 9-LOX products, which are usually associated with defence against pathogens, were exclusively activated by aphid attack.Furthermore, a correlation between volatiles and JA accumulation and the evolution of their precursors was determined.

View Article: PubMed Central - PubMed

Affiliation: Plant Biology Unit, Gembloux Agricultural University, Passage des déportés 2, B-5030 Gembloux, Belgium.

ABSTRACT
Plant defensive strategies bring into play blends of compounds dependent on the type of attacker and coming from different synthesis pathways. Interest in the field is mainly focused on volatile organic compounds (VOCs) and jasmonic acid (JA). By contrast, little is known about the oxidized polyunsaturated fatty acids (PUFAs), such as PUFA-hydroperoxides, PUFA-hydroxides, or PUFA-ketones. PUFA-hydroperoxides and their derivatives might be involved in stress response and show antimicrobial activities. Hydroperoxides are also precursors of JA and some volatile compounds. In this paper, the differential biochemical response of a plant against insects with distinct feeding behaviours is characterized not only in terms of VOC signature and JA profile but also in terms of their precursors synthesized through the lipoxygenase (LOX)-pathway at the early stage of the plant response. For this purpose, two leading pests of potato with distinct feeding behaviours were used: the Colorado Potato Beetle (Leptinotarsa decemlineata Say), a chewing herbivore, and the Green Peach Aphid (Myzus persicae Sulzer), a piercing-sucking insect. The volatile signatures identified clearly differ in function with the feeding behaviour of the attacker and the aphid, which causes the smaller damages, triggers the emission of a higher number of volatiles. In addition, 9-LOX products, which are usually associated with defence against pathogens, were exclusively activated by aphid attack. Furthermore, a correlation between volatiles and JA accumulation and the evolution of their precursors was determined. Finally, the role of the insect itself on the plant response after insect infestation was highlighted.

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(A) Ratio between JA content 8 h, 24 h, 48 h, and 72 h after insect attack and in control plants. (B) Ratio between OPDA content 8 h, 24 h, 48 h, and 72 h after insect attack and in control plants. The data are the average of two repetitions and are expressed as a ratio between the oxylipin concentration 8 h, 24 h, 48 h or 72 h after the beginning of the experiment and the oxylipin concentration in control plants (0 h). The values correspond to the mean and the error bars to the standard deviations. After one-way variance analysis means were classified using Student's t test. Differences between means were considered to be significantly different at P <0.05.
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fig4: (A) Ratio between JA content 8 h, 24 h, 48 h, and 72 h after insect attack and in control plants. (B) Ratio between OPDA content 8 h, 24 h, 48 h, and 72 h after insect attack and in control plants. The data are the average of two repetitions and are expressed as a ratio between the oxylipin concentration 8 h, 24 h, 48 h or 72 h after the beginning of the experiment and the oxylipin concentration in control plants (0 h). The values correspond to the mean and the error bars to the standard deviations. After one-way variance analysis means were classified using Student's t test. Differences between means were considered to be significantly different at P <0.05.

Mentions: JA plays a key role in the signal transduction pathway resulting in the activation of the induced defence response against both insects and pathogens. Indeed, JA-deficient plants are usually more susceptible towards herbivore attacks (Kessler et al., 2004; Howe and Jander, 2008) and pathogens (Staswick et al., 1998). The content of JA and its direct precursor OPDA was monitored in control plants and after 8 h, 24 h, 48 h, and 72 h after infestation with aphids or CPB. A significant increase in JA synthesis was only observed for aphid-infested plants 72 h after infestation (Fig. 4A). However, JA content was about twice as high in aphid-infested potato plants after 8 h (0.031±0.006 nmol g−1 FW) than in control plants (0.016±0.005 nmol g−1 FW) and the JA level in infested potato plants was relatively stable through the kinetics (at 24 h: 0.033±0.001 nmol g−1 FW, at 48 h: 0.038±0.008 nmol g−1 FW, at 72 h: 0.038±0.011 nmol g−1 FW). In addition, the accumulation of JA was higher in aphid-infested potato plants than in CPB-infested potato plants. OPDA content after 24 h (0.135 nmol g−1 FW) and 72 h (0.186 nmol g−1 FW) of infestation with aphids was significantly higher (P=0.001) than in control plants (0.062 nmol g−1 FW). By contrast, the OPDA content in CPB-infested plants was not significantly higher than in control plants (Fig. 4B) at the studied time points and JA and OPDA synthesis was mainly activated after aphid feeding.


Attacks by a piercing-sucking insect (Myzus persicae Sultzer) or a chewing insect (Leptinotarsa decemlineata Say) on potato plants (Solanum tuberosum L.) induce differential changes in volatile compound release and oxylipin synthesis.

Gosset V, Harmel N, Göbel C, Francis F, Haubruge E, Wathelet JP, du Jardin P, Feussner I, Fauconnier ML - J. Exp. Bot. (2009)

(A) Ratio between JA content 8 h, 24 h, 48 h, and 72 h after insect attack and in control plants. (B) Ratio between OPDA content 8 h, 24 h, 48 h, and 72 h after insect attack and in control plants. The data are the average of two repetitions and are expressed as a ratio between the oxylipin concentration 8 h, 24 h, 48 h or 72 h after the beginning of the experiment and the oxylipin concentration in control plants (0 h). The values correspond to the mean and the error bars to the standard deviations. After one-way variance analysis means were classified using Student's t test. Differences between means were considered to be significantly different at P <0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2657539&req=5

fig4: (A) Ratio between JA content 8 h, 24 h, 48 h, and 72 h after insect attack and in control plants. (B) Ratio between OPDA content 8 h, 24 h, 48 h, and 72 h after insect attack and in control plants. The data are the average of two repetitions and are expressed as a ratio between the oxylipin concentration 8 h, 24 h, 48 h or 72 h after the beginning of the experiment and the oxylipin concentration in control plants (0 h). The values correspond to the mean and the error bars to the standard deviations. After one-way variance analysis means were classified using Student's t test. Differences between means were considered to be significantly different at P <0.05.
Mentions: JA plays a key role in the signal transduction pathway resulting in the activation of the induced defence response against both insects and pathogens. Indeed, JA-deficient plants are usually more susceptible towards herbivore attacks (Kessler et al., 2004; Howe and Jander, 2008) and pathogens (Staswick et al., 1998). The content of JA and its direct precursor OPDA was monitored in control plants and after 8 h, 24 h, 48 h, and 72 h after infestation with aphids or CPB. A significant increase in JA synthesis was only observed for aphid-infested plants 72 h after infestation (Fig. 4A). However, JA content was about twice as high in aphid-infested potato plants after 8 h (0.031±0.006 nmol g−1 FW) than in control plants (0.016±0.005 nmol g−1 FW) and the JA level in infested potato plants was relatively stable through the kinetics (at 24 h: 0.033±0.001 nmol g−1 FW, at 48 h: 0.038±0.008 nmol g−1 FW, at 72 h: 0.038±0.011 nmol g−1 FW). In addition, the accumulation of JA was higher in aphid-infested potato plants than in CPB-infested potato plants. OPDA content after 24 h (0.135 nmol g−1 FW) and 72 h (0.186 nmol g−1 FW) of infestation with aphids was significantly higher (P=0.001) than in control plants (0.062 nmol g−1 FW). By contrast, the OPDA content in CPB-infested plants was not significantly higher than in control plants (Fig. 4B) at the studied time points and JA and OPDA synthesis was mainly activated after aphid feeding.

Bottom Line: Hydroperoxides are also precursors of JA and some volatile compounds.In addition, 9-LOX products, which are usually associated with defence against pathogens, were exclusively activated by aphid attack.Furthermore, a correlation between volatiles and JA accumulation and the evolution of their precursors was determined.

View Article: PubMed Central - PubMed

Affiliation: Plant Biology Unit, Gembloux Agricultural University, Passage des déportés 2, B-5030 Gembloux, Belgium.

ABSTRACT
Plant defensive strategies bring into play blends of compounds dependent on the type of attacker and coming from different synthesis pathways. Interest in the field is mainly focused on volatile organic compounds (VOCs) and jasmonic acid (JA). By contrast, little is known about the oxidized polyunsaturated fatty acids (PUFAs), such as PUFA-hydroperoxides, PUFA-hydroxides, or PUFA-ketones. PUFA-hydroperoxides and their derivatives might be involved in stress response and show antimicrobial activities. Hydroperoxides are also precursors of JA and some volatile compounds. In this paper, the differential biochemical response of a plant against insects with distinct feeding behaviours is characterized not only in terms of VOC signature and JA profile but also in terms of their precursors synthesized through the lipoxygenase (LOX)-pathway at the early stage of the plant response. For this purpose, two leading pests of potato with distinct feeding behaviours were used: the Colorado Potato Beetle (Leptinotarsa decemlineata Say), a chewing herbivore, and the Green Peach Aphid (Myzus persicae Sulzer), a piercing-sucking insect. The volatile signatures identified clearly differ in function with the feeding behaviour of the attacker and the aphid, which causes the smaller damages, triggers the emission of a higher number of volatiles. In addition, 9-LOX products, which are usually associated with defence against pathogens, were exclusively activated by aphid attack. Furthermore, a correlation between volatiles and JA accumulation and the evolution of their precursors was determined. Finally, the role of the insect itself on the plant response after insect infestation was highlighted.

Show MeSH
Related in: MedlinePlus