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Fingerprinting antioxidative activities in plants.

Saleh L, Plieth C - Plant Methods (2009)

Bottom Line: The intention of this work is to reduce the number of metabolic processes studied to a minimum of relevant parameters with a maximum yield of information.TAC runs in parallel with GR.It is inexpensive, quick and reproducible and delivers quantitative data. 'Summary parameters' like TAC, LUPO, and SOSA are quantitative traits which may be promising for implementation in high-throughput screening for robustness of novel mutants, transgenics, or breeds.

View Article: PubMed Central - HTML - PubMed

Affiliation: Zentrum für Biochemie und Molekularbiologie, Universität Kiel, Am Botanischen Garten 9, 24118 Kiel, Germany. cplieth@zbm.uni-kiel.de.

ABSTRACT

Background: A plethora of concurrent cellular activities is mobilised in the adaptation of plants to adverse environmental conditions. This response can be quantified by physiological experiments or metabolic profiling. The intention of this work is to reduce the number of metabolic processes studied to a minimum of relevant parameters with a maximum yield of information. Therefore, we inspected 'summary parameters' characteristic for whole classes of antioxidative metabolites and key enzymes.

Results: Three bioluminescence assays are presented. A horseradish peroxidase-based total antioxidative capacity (TAC) assay is used to probe low molecular weight antioxidants. Peroxidases are quantified by their luminol converting activity (LUPO). Finally, we quantify high molecular weight superoxide anion scavenging activity (SOSA) using coelenterazine.Experiments with Lepidium sativum L. show how salt, drought, cold, and heat influence the antioxidative system represented here by TAC, LUPO, SOSA, catalase, and glutathione reductase (GR). LUPO and SOSA run anti-parallel under all investigated stress conditions suggesting shifts in antioxidative functions rather than formation of antioxidative power. TAC runs in parallel with GR. This indicates that a majority of low molecular weight antioxidants in plants is represented by glutathione.

Conclusion: The set of assays presented here is capable of characterising antioxidative activities in plants. It is inexpensive, quick and reproducible and delivers quantitative data. 'Summary parameters' like TAC, LUPO, and SOSA are quantitative traits which may be promising for implementation in high-throughput screening for robustness of novel mutants, transgenics, or breeds.

No MeSH data available.


Related in: MedlinePlus

Drought-induced alterations in the antioxidative system of Lepidium sativum. Drought was induced by withdrawal of nutrient medium for 24 h and antioxidative parameters were measured (black bars) in comparison to untreated plants (white bars). The grey bars represent the recovery status 24 h after restoring the nutrient medium. Data presentation as in Figure 5.
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Figure 8: Drought-induced alterations in the antioxidative system of Lepidium sativum. Drought was induced by withdrawal of nutrient medium for 24 h and antioxidative parameters were measured (black bars) in comparison to untreated plants (white bars). The grey bars represent the recovery status 24 h after restoring the nutrient medium. Data presentation as in Figure 5.

Mentions: Fingerprints of antioxidative activities in Lepidium sativum after abiotic stress. The percentage change in the five screened parameters is represented by the five radial axes (data from Figures 5F, 6F, 7F, and 8F). The red pentagon in each panel represents the line of no change. Black polygons represent the antioxidative status immediately after stress treatment. Grey polygons represent the status after stress recovery. Each point is average of 5 technical replicates run on biological material pooled from 3 independent experiments. Standard deviations are given in Figures 5F–8F.


Fingerprinting antioxidative activities in plants.

Saleh L, Plieth C - Plant Methods (2009)

Drought-induced alterations in the antioxidative system of Lepidium sativum. Drought was induced by withdrawal of nutrient medium for 24 h and antioxidative parameters were measured (black bars) in comparison to untreated plants (white bars). The grey bars represent the recovery status 24 h after restoring the nutrient medium. Data presentation as in Figure 5.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2656482&req=5

Figure 8: Drought-induced alterations in the antioxidative system of Lepidium sativum. Drought was induced by withdrawal of nutrient medium for 24 h and antioxidative parameters were measured (black bars) in comparison to untreated plants (white bars). The grey bars represent the recovery status 24 h after restoring the nutrient medium. Data presentation as in Figure 5.
Mentions: Fingerprints of antioxidative activities in Lepidium sativum after abiotic stress. The percentage change in the five screened parameters is represented by the five radial axes (data from Figures 5F, 6F, 7F, and 8F). The red pentagon in each panel represents the line of no change. Black polygons represent the antioxidative status immediately after stress treatment. Grey polygons represent the status after stress recovery. Each point is average of 5 technical replicates run on biological material pooled from 3 independent experiments. Standard deviations are given in Figures 5F–8F.

Bottom Line: The intention of this work is to reduce the number of metabolic processes studied to a minimum of relevant parameters with a maximum yield of information.TAC runs in parallel with GR.It is inexpensive, quick and reproducible and delivers quantitative data. 'Summary parameters' like TAC, LUPO, and SOSA are quantitative traits which may be promising for implementation in high-throughput screening for robustness of novel mutants, transgenics, or breeds.

View Article: PubMed Central - HTML - PubMed

Affiliation: Zentrum für Biochemie und Molekularbiologie, Universität Kiel, Am Botanischen Garten 9, 24118 Kiel, Germany. cplieth@zbm.uni-kiel.de.

ABSTRACT

Background: A plethora of concurrent cellular activities is mobilised in the adaptation of plants to adverse environmental conditions. This response can be quantified by physiological experiments or metabolic profiling. The intention of this work is to reduce the number of metabolic processes studied to a minimum of relevant parameters with a maximum yield of information. Therefore, we inspected 'summary parameters' characteristic for whole classes of antioxidative metabolites and key enzymes.

Results: Three bioluminescence assays are presented. A horseradish peroxidase-based total antioxidative capacity (TAC) assay is used to probe low molecular weight antioxidants. Peroxidases are quantified by their luminol converting activity (LUPO). Finally, we quantify high molecular weight superoxide anion scavenging activity (SOSA) using coelenterazine.Experiments with Lepidium sativum L. show how salt, drought, cold, and heat influence the antioxidative system represented here by TAC, LUPO, SOSA, catalase, and glutathione reductase (GR). LUPO and SOSA run anti-parallel under all investigated stress conditions suggesting shifts in antioxidative functions rather than formation of antioxidative power. TAC runs in parallel with GR. This indicates that a majority of low molecular weight antioxidants in plants is represented by glutathione.

Conclusion: The set of assays presented here is capable of characterising antioxidative activities in plants. It is inexpensive, quick and reproducible and delivers quantitative data. 'Summary parameters' like TAC, LUPO, and SOSA are quantitative traits which may be promising for implementation in high-throughput screening for robustness of novel mutants, transgenics, or breeds.

No MeSH data available.


Related in: MedlinePlus