Limits...
The uterine expression of SEC63 gene is up-regulated at implantation sites in association with the decidualization during the early pregnancy in mice.

Su RW, Sun ZG, Zhao YC, Chen QJ, Yang ZM, Li RS, Wang J - Reprod. Biol. Endocrinol. (2009)

Bottom Line: Sec63 is a key component of the protein translocation machinery in the mammalian endoplasmic reticulum (ER), and involved in the post-translation processing of secretory proteins.The presence of Sec63 protein in human uterine tissue was also detected by immunohistochemical analysis.Statistical analysis was carried out using Tukey test.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Life Science, Xiamen University, Xiamen 361005, PR China. foolishwolfsu@yahoo.com.cn

ABSTRACT

Background: Sec63 is a key component of the protein translocation machinery in the mammalian endoplasmic reticulum (ER), and involved in the post-translation processing of secretory proteins. The aim of this study was to determine the expression pattern of SEC63 gene in mouse uterus during the early pregnancy.

Methods: Real-time quantitative PCR and Western blot analyses were used to evaluate the alteration in levels of uterine SEC63 gene expression during the peri-implantation period in mice. Further, both in situ hybridization and immunohistochemical analyses were performed to examine the spatial localization of SEC63 gene expression in mouse uterine tissues. The presence of Sec63 protein in human uterine tissue was also detected by immunohistochemical analysis. Statistical analysis was carried out using Tukey test.

Results: Uterine SEC63 gene expression was up-regulated and predominantly localized in mouse decidual cells during days 5-8 of pregnancy. More interestingly, Sec63 protein was also detected in human decidua of 10-week pregnancy, whereas was not observed in human endometrial tissues both at proliferative and secretory phases of menstrual cycle.

Conclusion: The pattern of SEC63 gene expression is consistent with a possible role for SEC63 in decidualization.

Show MeSH

Related in: MedlinePlus

Uterine expression level of Sec63 mRNA during the early pregnancy. Real-time quantitative PCR analysis of the expression level of Sec63 mRNA in mouse uterine tissues of day 4 (4), day 5 (5) and day 7 (7) pregnancy (panel A), as well as the inter-implantation sites (nI), implantation sites (I) and embryo (Em) of day 7 pregnancy (panel B), by using the β-Actin as the housekeeping gene. In panel A and panel B, the data were normalized relative to that of day 4 and nI (reference groups) respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2655295&req=5

Figure 2: Uterine expression level of Sec63 mRNA during the early pregnancy. Real-time quantitative PCR analysis of the expression level of Sec63 mRNA in mouse uterine tissues of day 4 (4), day 5 (5) and day 7 (7) pregnancy (panel A), as well as the inter-implantation sites (nI), implantation sites (I) and embryo (Em) of day 7 pregnancy (panel B), by using the β-Actin as the housekeeping gene. In panel A and panel B, the data were normalized relative to that of day 4 and nI (reference groups) respectively.

Mentions: The uterine expression levels of SEC63 mRNA of days 4, 5 and 7 were determined by real-time quantitative PCR analysis (See additional file 1 for Melting curve of real-time quantitative PCR analysis for Sec63 mRNA). Compared to that of day 4 pregnant mouse, the measured values of SEC63 mRNA in the mouse uterus on days 5 and 7 of pregnancy were significantly increased (Fig. 2A and Table 1). Furthermore, the expression level of SEC63 mRNA was up-regulated in tendency at the implantation sites compared to the inter-implantation sites on day 7 pregnancy (Fig. 2B), despite of the great variance possibly resulting from the dissection operation of the implantation site and the non-implantation site.


The uterine expression of SEC63 gene is up-regulated at implantation sites in association with the decidualization during the early pregnancy in mice.

Su RW, Sun ZG, Zhao YC, Chen QJ, Yang ZM, Li RS, Wang J - Reprod. Biol. Endocrinol. (2009)

Uterine expression level of Sec63 mRNA during the early pregnancy. Real-time quantitative PCR analysis of the expression level of Sec63 mRNA in mouse uterine tissues of day 4 (4), day 5 (5) and day 7 (7) pregnancy (panel A), as well as the inter-implantation sites (nI), implantation sites (I) and embryo (Em) of day 7 pregnancy (panel B), by using the β-Actin as the housekeeping gene. In panel A and panel B, the data were normalized relative to that of day 4 and nI (reference groups) respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2655295&req=5

Figure 2: Uterine expression level of Sec63 mRNA during the early pregnancy. Real-time quantitative PCR analysis of the expression level of Sec63 mRNA in mouse uterine tissues of day 4 (4), day 5 (5) and day 7 (7) pregnancy (panel A), as well as the inter-implantation sites (nI), implantation sites (I) and embryo (Em) of day 7 pregnancy (panel B), by using the β-Actin as the housekeeping gene. In panel A and panel B, the data were normalized relative to that of day 4 and nI (reference groups) respectively.
Mentions: The uterine expression levels of SEC63 mRNA of days 4, 5 and 7 were determined by real-time quantitative PCR analysis (See additional file 1 for Melting curve of real-time quantitative PCR analysis for Sec63 mRNA). Compared to that of day 4 pregnant mouse, the measured values of SEC63 mRNA in the mouse uterus on days 5 and 7 of pregnancy were significantly increased (Fig. 2A and Table 1). Furthermore, the expression level of SEC63 mRNA was up-regulated in tendency at the implantation sites compared to the inter-implantation sites on day 7 pregnancy (Fig. 2B), despite of the great variance possibly resulting from the dissection operation of the implantation site and the non-implantation site.

Bottom Line: Sec63 is a key component of the protein translocation machinery in the mammalian endoplasmic reticulum (ER), and involved in the post-translation processing of secretory proteins.The presence of Sec63 protein in human uterine tissue was also detected by immunohistochemical analysis.Statistical analysis was carried out using Tukey test.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Life Science, Xiamen University, Xiamen 361005, PR China. foolishwolfsu@yahoo.com.cn

ABSTRACT

Background: Sec63 is a key component of the protein translocation machinery in the mammalian endoplasmic reticulum (ER), and involved in the post-translation processing of secretory proteins. The aim of this study was to determine the expression pattern of SEC63 gene in mouse uterus during the early pregnancy.

Methods: Real-time quantitative PCR and Western blot analyses were used to evaluate the alteration in levels of uterine SEC63 gene expression during the peri-implantation period in mice. Further, both in situ hybridization and immunohistochemical analyses were performed to examine the spatial localization of SEC63 gene expression in mouse uterine tissues. The presence of Sec63 protein in human uterine tissue was also detected by immunohistochemical analysis. Statistical analysis was carried out using Tukey test.

Results: Uterine SEC63 gene expression was up-regulated and predominantly localized in mouse decidual cells during days 5-8 of pregnancy. More interestingly, Sec63 protein was also detected in human decidua of 10-week pregnancy, whereas was not observed in human endometrial tissues both at proliferative and secretory phases of menstrual cycle.

Conclusion: The pattern of SEC63 gene expression is consistent with a possible role for SEC63 in decidualization.

Show MeSH
Related in: MedlinePlus