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The uterine expression of SEC63 gene is up-regulated at implantation sites in association with the decidualization during the early pregnancy in mice.

Su RW, Sun ZG, Zhao YC, Chen QJ, Yang ZM, Li RS, Wang J - Reprod. Biol. Endocrinol. (2009)

Bottom Line: Sec63 is a key component of the protein translocation machinery in the mammalian endoplasmic reticulum (ER), and involved in the post-translation processing of secretory proteins.The presence of Sec63 protein in human uterine tissue was also detected by immunohistochemical analysis.Statistical analysis was carried out using Tukey test.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Life Science, Xiamen University, Xiamen 361005, PR China. foolishwolfsu@yahoo.com.cn

ABSTRACT

Background: Sec63 is a key component of the protein translocation machinery in the mammalian endoplasmic reticulum (ER), and involved in the post-translation processing of secretory proteins. The aim of this study was to determine the expression pattern of SEC63 gene in mouse uterus during the early pregnancy.

Methods: Real-time quantitative PCR and Western blot analyses were used to evaluate the alteration in levels of uterine SEC63 gene expression during the peri-implantation period in mice. Further, both in situ hybridization and immunohistochemical analyses were performed to examine the spatial localization of SEC63 gene expression in mouse uterine tissues. The presence of Sec63 protein in human uterine tissue was also detected by immunohistochemical analysis. Statistical analysis was carried out using Tukey test.

Results: Uterine SEC63 gene expression was up-regulated and predominantly localized in mouse decidual cells during days 5-8 of pregnancy. More interestingly, Sec63 protein was also detected in human decidua of 10-week pregnancy, whereas was not observed in human endometrial tissues both at proliferative and secretory phases of menstrual cycle.

Conclusion: The pattern of SEC63 gene expression is consistent with a possible role for SEC63 in decidualization.

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Related in: MedlinePlus

The tissue distribution of Sec63 mRNA in the mouse. The tissue distribution of Sec63 mRNA in the mouse was analyzed by RT-PCR by using Gapdh mRNA as the control. Sec63 mRNA was detected in all of the tested tissues. (B: brain; Lu: lung; H: heart; Li: liver; Sp: spleen; K: kidney; M: muscle; SI: small intestine; Sk: skin; T: testis; E: epididymis; O: ovary; U: uterus.)
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Figure 1: The tissue distribution of Sec63 mRNA in the mouse. The tissue distribution of Sec63 mRNA in the mouse was analyzed by RT-PCR by using Gapdh mRNA as the control. Sec63 mRNA was detected in all of the tested tissues. (B: brain; Lu: lung; H: heart; Li: liver; Sp: spleen; K: kidney; M: muscle; SI: small intestine; Sk: skin; T: testis; E: epididymis; O: ovary; U: uterus.)

Mentions: Multi-tissue RT-PCR analysis was performed to observe the tissue distribution of the SEC63 gene expression in different mouse tissues using GAPDH as the housekeeping control. The results showed that SEC63 mRNA was widely expressed in all of the tissues tested (Fig. 1).


The uterine expression of SEC63 gene is up-regulated at implantation sites in association with the decidualization during the early pregnancy in mice.

Su RW, Sun ZG, Zhao YC, Chen QJ, Yang ZM, Li RS, Wang J - Reprod. Biol. Endocrinol. (2009)

The tissue distribution of Sec63 mRNA in the mouse. The tissue distribution of Sec63 mRNA in the mouse was analyzed by RT-PCR by using Gapdh mRNA as the control. Sec63 mRNA was detected in all of the tested tissues. (B: brain; Lu: lung; H: heart; Li: liver; Sp: spleen; K: kidney; M: muscle; SI: small intestine; Sk: skin; T: testis; E: epididymis; O: ovary; U: uterus.)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2655295&req=5

Figure 1: The tissue distribution of Sec63 mRNA in the mouse. The tissue distribution of Sec63 mRNA in the mouse was analyzed by RT-PCR by using Gapdh mRNA as the control. Sec63 mRNA was detected in all of the tested tissues. (B: brain; Lu: lung; H: heart; Li: liver; Sp: spleen; K: kidney; M: muscle; SI: small intestine; Sk: skin; T: testis; E: epididymis; O: ovary; U: uterus.)
Mentions: Multi-tissue RT-PCR analysis was performed to observe the tissue distribution of the SEC63 gene expression in different mouse tissues using GAPDH as the housekeeping control. The results showed that SEC63 mRNA was widely expressed in all of the tissues tested (Fig. 1).

Bottom Line: Sec63 is a key component of the protein translocation machinery in the mammalian endoplasmic reticulum (ER), and involved in the post-translation processing of secretory proteins.The presence of Sec63 protein in human uterine tissue was also detected by immunohistochemical analysis.Statistical analysis was carried out using Tukey test.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Life Science, Xiamen University, Xiamen 361005, PR China. foolishwolfsu@yahoo.com.cn

ABSTRACT

Background: Sec63 is a key component of the protein translocation machinery in the mammalian endoplasmic reticulum (ER), and involved in the post-translation processing of secretory proteins. The aim of this study was to determine the expression pattern of SEC63 gene in mouse uterus during the early pregnancy.

Methods: Real-time quantitative PCR and Western blot analyses were used to evaluate the alteration in levels of uterine SEC63 gene expression during the peri-implantation period in mice. Further, both in situ hybridization and immunohistochemical analyses were performed to examine the spatial localization of SEC63 gene expression in mouse uterine tissues. The presence of Sec63 protein in human uterine tissue was also detected by immunohistochemical analysis. Statistical analysis was carried out using Tukey test.

Results: Uterine SEC63 gene expression was up-regulated and predominantly localized in mouse decidual cells during days 5-8 of pregnancy. More interestingly, Sec63 protein was also detected in human decidua of 10-week pregnancy, whereas was not observed in human endometrial tissues both at proliferative and secretory phases of menstrual cycle.

Conclusion: The pattern of SEC63 gene expression is consistent with a possible role for SEC63 in decidualization.

Show MeSH
Related in: MedlinePlus