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In vitro drug release behavior from a novel thermosensitive composite hydrogel based on Pluronic f127 and poly(ethylene glycol)-poly(epsilon-caprolactone)-poly(ethylene glycol) copolymer.

Gong CY, Shi S, Dong PW, Zheng XL, Fu SZ, Guo G, Yang JL, Wei YQ, Qian ZY - BMC Biotechnol. (2009)

Bottom Line: Chemical composition of composite hydrogel, initial drug loading, and hydrogel concentration substantially affected the drug release behavior.The higher Pluronic F127 content, lower initial drug loading amount, or lower hydrogel concentration resulted in higher cumulative release rate.The results showed that composite hydrogel prepared in this paper were biocompatible with low cell cytotoxicity, and the drugs in this work could be released slowly from composite hydrogel in an extended period, which suggested that the composite hydrogel might have great potential applications in biomedical fields.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu, 610041, PR China. chygong14@yahoo.com.cn

ABSTRACT

Background: Most conventional methods for delivering chemotherapeutic agents fail to achieve therapeutic concentrations of drugs, despite reaching toxic systemic levels. Novel controlled drug delivery systems are designed to deliver drugs at predetermined rates for predefined periods at the target organ and overcome the shortcomings of conventional drug formulations therefore could diminish the side effects and improve the life quality of the patients. Thus, a suitable controlled drug delivery system is extremely important for chemotherapy.

Results: A novel biodegradable thermosensitive composite hydrogel, based on poly(ethylene glycol)-poly(epsilon-caprolactone)-poly(ethylene glycol) (PEG-PCL-PEG, PECE) and Pluronic F127 copolymer, was successfully prepared in this work, which underwent thermosensitive sol-gel-sol transition. And it was flowing sol at ambient temperature but became non-flowing gel at body temperature. By varying the composition, sol-gel-sol transition and in vitro drug release behavior of the composite hydrogel could be adjusted. Cytotoxicity of the composite hydrogel was conducted by cell viability assay using human HEK293 cells. The 293 cell viability of composite hydrogel copolymers were yet higher than 71.4%, even when the input copolymers were 500 microg per well. Vitamin B12 (VB12), honokiol (HK), and bovine serum albumin (BSA) were used as model drugs to investigate the in vitro release behavior of hydrophilic small molecular drug, hydrophobic small molecular drug, and protein drug from the composite hydrogel respectively. All the above-mentioned drugs in this work could be released slowly from composite hydrogel in an extended period. Chemical composition of composite hydrogel, initial drug loading, and hydrogel concentration substantially affected the drug release behavior. The higher Pluronic F127 content, lower initial drug loading amount, or lower hydrogel concentration resulted in higher cumulative release rate.

Conclusion: The results showed that composite hydrogel prepared in this paper were biocompatible with low cell cytotoxicity, and the drugs in this work could be released slowly from composite hydrogel in an extended period, which suggested that the composite hydrogel might have great potential applications in biomedical fields.

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Related in: MedlinePlus

In vitro release behavior of HK from composite hydrogel. A: release behavior of S1, S3, an S6 hydrogel with the same hydrogel concentration (30 wt%) and initial drug loading amount (1 mg). B: release behavior of 30 wt% S4 hydrogel with different initial drug loading amount (1 mg and 2 mg). C: release behavior of 1 mg HK loaded S4 hydrogel with different hydrogel concentration (20 wt% and 30 wt%). Error bars represent the standard deviation (n = 3).
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Figure 6: In vitro release behavior of HK from composite hydrogel. A: release behavior of S1, S3, an S6 hydrogel with the same hydrogel concentration (30 wt%) and initial drug loading amount (1 mg). B: release behavior of 30 wt% S4 hydrogel with different initial drug loading amount (1 mg and 2 mg). C: release behavior of 1 mg HK loaded S4 hydrogel with different hydrogel concentration (20 wt% and 30 wt%). Error bars represent the standard deviation (n = 3).

Mentions: The release behavior of HK from composite hydrogel was performed and the cumulative release profile was presented in Fig. 6. In Fig. 6–A, with increase in content of Pluronic F127 copolymer from 0% (S1) to 100% (S6), cumulative release rate and burst release rate (in one hour) increased from 37.1% to 86.5% and from 1.4% to 8.9%, respectively. According to Fig. 6–B, with increase of initial drug loading amount, the cumulative release rate of HK decreased dramatically from 62.1% to 51.0% in a 14-day period. As shown in Fig. 6–C, lower concentration of composite hydrogel led to higher cumulative release rate in a shorter time. Compared with VB12release profile, cumulative release rate and burst release rate of HK were much lower, which should be contributed to the high hydrophobicity of HK.


In vitro drug release behavior from a novel thermosensitive composite hydrogel based on Pluronic f127 and poly(ethylene glycol)-poly(epsilon-caprolactone)-poly(ethylene glycol) copolymer.

Gong CY, Shi S, Dong PW, Zheng XL, Fu SZ, Guo G, Yang JL, Wei YQ, Qian ZY - BMC Biotechnol. (2009)

In vitro release behavior of HK from composite hydrogel. A: release behavior of S1, S3, an S6 hydrogel with the same hydrogel concentration (30 wt%) and initial drug loading amount (1 mg). B: release behavior of 30 wt% S4 hydrogel with different initial drug loading amount (1 mg and 2 mg). C: release behavior of 1 mg HK loaded S4 hydrogel with different hydrogel concentration (20 wt% and 30 wt%). Error bars represent the standard deviation (n = 3).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2654890&req=5

Figure 6: In vitro release behavior of HK from composite hydrogel. A: release behavior of S1, S3, an S6 hydrogel with the same hydrogel concentration (30 wt%) and initial drug loading amount (1 mg). B: release behavior of 30 wt% S4 hydrogel with different initial drug loading amount (1 mg and 2 mg). C: release behavior of 1 mg HK loaded S4 hydrogel with different hydrogel concentration (20 wt% and 30 wt%). Error bars represent the standard deviation (n = 3).
Mentions: The release behavior of HK from composite hydrogel was performed and the cumulative release profile was presented in Fig. 6. In Fig. 6–A, with increase in content of Pluronic F127 copolymer from 0% (S1) to 100% (S6), cumulative release rate and burst release rate (in one hour) increased from 37.1% to 86.5% and from 1.4% to 8.9%, respectively. According to Fig. 6–B, with increase of initial drug loading amount, the cumulative release rate of HK decreased dramatically from 62.1% to 51.0% in a 14-day period. As shown in Fig. 6–C, lower concentration of composite hydrogel led to higher cumulative release rate in a shorter time. Compared with VB12release profile, cumulative release rate and burst release rate of HK were much lower, which should be contributed to the high hydrophobicity of HK.

Bottom Line: Chemical composition of composite hydrogel, initial drug loading, and hydrogel concentration substantially affected the drug release behavior.The higher Pluronic F127 content, lower initial drug loading amount, or lower hydrogel concentration resulted in higher cumulative release rate.The results showed that composite hydrogel prepared in this paper were biocompatible with low cell cytotoxicity, and the drugs in this work could be released slowly from composite hydrogel in an extended period, which suggested that the composite hydrogel might have great potential applications in biomedical fields.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu, 610041, PR China. chygong14@yahoo.com.cn

ABSTRACT

Background: Most conventional methods for delivering chemotherapeutic agents fail to achieve therapeutic concentrations of drugs, despite reaching toxic systemic levels. Novel controlled drug delivery systems are designed to deliver drugs at predetermined rates for predefined periods at the target organ and overcome the shortcomings of conventional drug formulations therefore could diminish the side effects and improve the life quality of the patients. Thus, a suitable controlled drug delivery system is extremely important for chemotherapy.

Results: A novel biodegradable thermosensitive composite hydrogel, based on poly(ethylene glycol)-poly(epsilon-caprolactone)-poly(ethylene glycol) (PEG-PCL-PEG, PECE) and Pluronic F127 copolymer, was successfully prepared in this work, which underwent thermosensitive sol-gel-sol transition. And it was flowing sol at ambient temperature but became non-flowing gel at body temperature. By varying the composition, sol-gel-sol transition and in vitro drug release behavior of the composite hydrogel could be adjusted. Cytotoxicity of the composite hydrogel was conducted by cell viability assay using human HEK293 cells. The 293 cell viability of composite hydrogel copolymers were yet higher than 71.4%, even when the input copolymers were 500 microg per well. Vitamin B12 (VB12), honokiol (HK), and bovine serum albumin (BSA) were used as model drugs to investigate the in vitro release behavior of hydrophilic small molecular drug, hydrophobic small molecular drug, and protein drug from the composite hydrogel respectively. All the above-mentioned drugs in this work could be released slowly from composite hydrogel in an extended period. Chemical composition of composite hydrogel, initial drug loading, and hydrogel concentration substantially affected the drug release behavior. The higher Pluronic F127 content, lower initial drug loading amount, or lower hydrogel concentration resulted in higher cumulative release rate.

Conclusion: The results showed that composite hydrogel prepared in this paper were biocompatible with low cell cytotoxicity, and the drugs in this work could be released slowly from composite hydrogel in an extended period, which suggested that the composite hydrogel might have great potential applications in biomedical fields.

Show MeSH
Related in: MedlinePlus