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Myeloid-related protein 8/14 complex describes microcirculatory alterations in patients with type 2 diabetes and nephropathy.

Burkhardt K, Schwarz S, Pan C, Stelter F, Kotliar K, Von Eynatten M, Sollinger D, Lanzl I, Heemann U, Baumann M - Cardiovasc Diabetol (2009)

Bottom Line: MRP8/14 was positively associated with inflammation (r = 0.57), proteinuria (r = 0.40) and retinal arterial caliber (r = 0.48).Both groups did not differ with regard to metabolic factors and blood pressure.MRP8/14 was an independent predictor of retinal artery caliber in multivariate stepwise regression analysis (beta = 0.607) and was positively associated with IL-6 (r = 0.57, P < 0.001) and TNF-alpha (r = 0.36, P < 0.05).

View Article: PubMed Central - HTML - PubMed

Affiliation: Nephrological Clinic Weissenburg, 91781 Weissenburg, Germany. praxis-dr-burkhardt@t-online.de

ABSTRACT

Background: Inflammation contributes to cardiovascular complications in type 2 diabetes, which are often characterized by microvascular alterations. We investigated whether myeloid-related protein 8/14 complex (MRP8/14) secreted by transmigrating monocytes and granulocytes may represent a biomarker for microvascular alterations in patients with type 2 diabetes and nephropathy.

Methods: MRP8/14 was measured in 43 patients with type 2 diabetes and nephropathy. Additionally, the inflammatory markers Interleukin-6 (IL-6), Tumor necrosis factor-alpha (TNF-alpha) and C-reactive protein (CRP) were quantified. To detect microvascular alterations proteinuria and retinal vessel caliber were used as classical and novel marker, respectively. Proteinuria was quantified by protein-creatinine ratio (PCR); retinal vessel caliber was quantified after retina photography on digitalized retina pictures.

Results: MRP8/14 was positively associated with inflammation (r = 0.57), proteinuria (r = 0.40) and retinal arterial caliber (r = 0.48). Type 2 diabetic patients with MRP8/14 values above the median of 5.8 microg/ml demonstrated higher proteinuria and larger retinal artery caliber than patients with MRP8/14 values below the median (logPCR: -0.51 +/- 0.52 versus -0.96 +/- 0.46, P < 0.01; retinal artery lumen (microm): 178.3 +/- 14.1 versus 162.7 +/- 14.9 P < 0.01). Both groups did not differ with regard to metabolic factors and blood pressure. MRP8/14 was an independent predictor of retinal artery caliber in multivariate stepwise regression analysis (beta = 0.607) and was positively associated with IL-6 (r = 0.57, P < 0.001) and TNF-alpha (r = 0.36, P < 0.05).

Conclusion: MRP8/14--a marker for transendothelial migration--describes not only the state of inflammation in diabetic nephropathy, but additionally the degree of microvascular alterations in the glomerular and retinal bed. Therefore, MRP8/14 may be a potentially selective novel biomarker for microcirculatory defects in diabetic nephropathy.

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Bivariate correlations of Interleukin-6 (IL-6) and TNF-α with proteinuria and retinal artery caliber in patients with type 2 diabetes and nephropathy: A) IL-6 and proteinuria, B) IL-6 and retinal artery caliber, C) TNF-α and proteinuria and D) TNF-α and retinal artery caliber. We observed significant positive correlations for A) r = 0.39, B) r = 0.42 and D) r = 0.41. All correlations are given as calculated by Spearman correlation coefficient.
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Figure 1: Bivariate correlations of Interleukin-6 (IL-6) and TNF-α with proteinuria and retinal artery caliber in patients with type 2 diabetes and nephropathy: A) IL-6 and proteinuria, B) IL-6 and retinal artery caliber, C) TNF-α and proteinuria and D) TNF-α and retinal artery caliber. We observed significant positive correlations for A) r = 0.39, B) r = 0.42 and D) r = 0.41. All correlations are given as calculated by Spearman correlation coefficient.

Mentions: Patients with diabetic nephropathy and proteinuria had a positive association between the degree of proteinuria and inflammation, although restricted to IL-6 (r = 0.39, P < 0.05, Figure 1). Other inflammatory parameters such as TNF-α and CRP were not significantly associated with the degree of proteinuria.


Myeloid-related protein 8/14 complex describes microcirculatory alterations in patients with type 2 diabetes and nephropathy.

Burkhardt K, Schwarz S, Pan C, Stelter F, Kotliar K, Von Eynatten M, Sollinger D, Lanzl I, Heemann U, Baumann M - Cardiovasc Diabetol (2009)

Bivariate correlations of Interleukin-6 (IL-6) and TNF-α with proteinuria and retinal artery caliber in patients with type 2 diabetes and nephropathy: A) IL-6 and proteinuria, B) IL-6 and retinal artery caliber, C) TNF-α and proteinuria and D) TNF-α and retinal artery caliber. We observed significant positive correlations for A) r = 0.39, B) r = 0.42 and D) r = 0.41. All correlations are given as calculated by Spearman correlation coefficient.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2654885&req=5

Figure 1: Bivariate correlations of Interleukin-6 (IL-6) and TNF-α with proteinuria and retinal artery caliber in patients with type 2 diabetes and nephropathy: A) IL-6 and proteinuria, B) IL-6 and retinal artery caliber, C) TNF-α and proteinuria and D) TNF-α and retinal artery caliber. We observed significant positive correlations for A) r = 0.39, B) r = 0.42 and D) r = 0.41. All correlations are given as calculated by Spearman correlation coefficient.
Mentions: Patients with diabetic nephropathy and proteinuria had a positive association between the degree of proteinuria and inflammation, although restricted to IL-6 (r = 0.39, P < 0.05, Figure 1). Other inflammatory parameters such as TNF-α and CRP were not significantly associated with the degree of proteinuria.

Bottom Line: MRP8/14 was positively associated with inflammation (r = 0.57), proteinuria (r = 0.40) and retinal arterial caliber (r = 0.48).Both groups did not differ with regard to metabolic factors and blood pressure.MRP8/14 was an independent predictor of retinal artery caliber in multivariate stepwise regression analysis (beta = 0.607) and was positively associated with IL-6 (r = 0.57, P < 0.001) and TNF-alpha (r = 0.36, P < 0.05).

View Article: PubMed Central - HTML - PubMed

Affiliation: Nephrological Clinic Weissenburg, 91781 Weissenburg, Germany. praxis-dr-burkhardt@t-online.de

ABSTRACT

Background: Inflammation contributes to cardiovascular complications in type 2 diabetes, which are often characterized by microvascular alterations. We investigated whether myeloid-related protein 8/14 complex (MRP8/14) secreted by transmigrating monocytes and granulocytes may represent a biomarker for microvascular alterations in patients with type 2 diabetes and nephropathy.

Methods: MRP8/14 was measured in 43 patients with type 2 diabetes and nephropathy. Additionally, the inflammatory markers Interleukin-6 (IL-6), Tumor necrosis factor-alpha (TNF-alpha) and C-reactive protein (CRP) were quantified. To detect microvascular alterations proteinuria and retinal vessel caliber were used as classical and novel marker, respectively. Proteinuria was quantified by protein-creatinine ratio (PCR); retinal vessel caliber was quantified after retina photography on digitalized retina pictures.

Results: MRP8/14 was positively associated with inflammation (r = 0.57), proteinuria (r = 0.40) and retinal arterial caliber (r = 0.48). Type 2 diabetic patients with MRP8/14 values above the median of 5.8 microg/ml demonstrated higher proteinuria and larger retinal artery caliber than patients with MRP8/14 values below the median (logPCR: -0.51 +/- 0.52 versus -0.96 +/- 0.46, P < 0.01; retinal artery lumen (microm): 178.3 +/- 14.1 versus 162.7 +/- 14.9 P < 0.01). Both groups did not differ with regard to metabolic factors and blood pressure. MRP8/14 was an independent predictor of retinal artery caliber in multivariate stepwise regression analysis (beta = 0.607) and was positively associated with IL-6 (r = 0.57, P < 0.001) and TNF-alpha (r = 0.36, P < 0.05).

Conclusion: MRP8/14--a marker for transendothelial migration--describes not only the state of inflammation in diabetic nephropathy, but additionally the degree of microvascular alterations in the glomerular and retinal bed. Therefore, MRP8/14 may be a potentially selective novel biomarker for microcirculatory defects in diabetic nephropathy.

Show MeSH
Related in: MedlinePlus