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Is the fetal lung a mineralocorticoid receptor target organ? Induction of cortisol-regulated genes in the ovine fetal lung, kidney and small intestine.

Keller-Wood M, von Reitzenstein M, McCartney J - Neonatology (2008)

Bottom Line: Expression of corticosteroid-induced genes in renal cortex increases at term; in small intestine the induction occurs postnatally.In contrast, in lung expression of MR and GR mRNAs were greater at 100 days to term than postnatally and 11 beta-HSD1 peaked at 145 days; the corticosteroid-induced genes also increased prenatally: Sgk-1 and ENaC alpha increased by 120 days, peaking at 145 days, and Na,K-ATPase alpha1 was greatest at 130 days.The expression of high levels of MR and 11 beta-HSD1 in preterm fetal lung suggest low endogenous fetal cortisol may exert actions at the high affinity MR in vivo, leading to increases in expression of sodium channels important in the regulation of lung liquid secretion and reabsorption.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacodynamics, University of Florida, Gainesville, FL 32610, USA. kellerwd@cop.ufl.edu

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Expression of MR and GR and their target genes in small intestine of 80-, 100-, 120-, 130- and 145-day fetuses and day 1 and day 7 neonatal lambs. Data depicted as mRNA fold changes relative to 80 days. Panels and symbols are as described in figure 1.
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Figure 4: Expression of MR and GR and their target genes in small intestine of 80-, 100-, 120-, 130- and 145-day fetuses and day 1 and day 7 neonatal lambs. Data depicted as mRNA fold changes relative to 80 days. Panels and symbols are as described in figure 1.

Mentions: GR was abundantly expressed in lungs, and renal cortex and medulla. Mean GR mRNA expression relative to 18S was approximately 8-fold greater in lung than in renal cortex, and almost 20-fold greater than in small intestine at 130 days’ gestation, however expression at postnatal day 7 was more similar among tissues (table 1). GR expression in the lung was significantly increased during the second half of fetal life and was significantly higher in fetal lung than in postnatal lung (fig. 1a). In renal cortex, GR expression increased by day 145, but decreased postnatally (fig. 2a). GR mRNA expression was not significantly different across gestational age in small intestine or renal medulla (fig. 3a, 4a). In contrast, GR expression in small intestine was significantly increased at postnatal day 7 (fig. 4a).


Is the fetal lung a mineralocorticoid receptor target organ? Induction of cortisol-regulated genes in the ovine fetal lung, kidney and small intestine.

Keller-Wood M, von Reitzenstein M, McCartney J - Neonatology (2008)

Expression of MR and GR and their target genes in small intestine of 80-, 100-, 120-, 130- and 145-day fetuses and day 1 and day 7 neonatal lambs. Data depicted as mRNA fold changes relative to 80 days. Panels and symbols are as described in figure 1.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2654587&req=5

Figure 4: Expression of MR and GR and their target genes in small intestine of 80-, 100-, 120-, 130- and 145-day fetuses and day 1 and day 7 neonatal lambs. Data depicted as mRNA fold changes relative to 80 days. Panels and symbols are as described in figure 1.
Mentions: GR was abundantly expressed in lungs, and renal cortex and medulla. Mean GR mRNA expression relative to 18S was approximately 8-fold greater in lung than in renal cortex, and almost 20-fold greater than in small intestine at 130 days’ gestation, however expression at postnatal day 7 was more similar among tissues (table 1). GR expression in the lung was significantly increased during the second half of fetal life and was significantly higher in fetal lung than in postnatal lung (fig. 1a). In renal cortex, GR expression increased by day 145, but decreased postnatally (fig. 2a). GR mRNA expression was not significantly different across gestational age in small intestine or renal medulla (fig. 3a, 4a). In contrast, GR expression in small intestine was significantly increased at postnatal day 7 (fig. 4a).

Bottom Line: Expression of corticosteroid-induced genes in renal cortex increases at term; in small intestine the induction occurs postnatally.In contrast, in lung expression of MR and GR mRNAs were greater at 100 days to term than postnatally and 11 beta-HSD1 peaked at 145 days; the corticosteroid-induced genes also increased prenatally: Sgk-1 and ENaC alpha increased by 120 days, peaking at 145 days, and Na,K-ATPase alpha1 was greatest at 130 days.The expression of high levels of MR and 11 beta-HSD1 in preterm fetal lung suggest low endogenous fetal cortisol may exert actions at the high affinity MR in vivo, leading to increases in expression of sodium channels important in the regulation of lung liquid secretion and reabsorption.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacodynamics, University of Florida, Gainesville, FL 32610, USA. kellerwd@cop.ufl.edu

Show MeSH
Related in: MedlinePlus