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Effect of Obstructive Jaundice and Nitric Oxide on the Profiles of Intestinal Bacterial Flora in Wild and iNOS Mice.

Hong JY, F Sato E, Nishikawa T, Hiramoto K, Inoue M - J Clin Biochem Nutr (2009)

Bottom Line: The amounts of bacterial DNA detected in fecal samples from both animal groups pretreated with antibiotics were extremely low as compared with untreated groups.We found that the profiles of enteric bacteria changed markedly after BDL.Among enteric bacteria examined, Lactobacillus murinus was found to increase markedly after BDL in rectum of both animal groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Pathology, Osaka City University Medical School, 1-4-3 Asahimachi, Abeno, Osaka 545-8585, Japan.

ABSTRACT
We previously reported that the plasma level of endotoxin and colonic expression of IgA in the mouse increased with obstructive jaundice induced by bile duct ligation (BDL). To elucidate the mechanism of the BDL-induced increase, we analyzed the effect of BDL on intestinal flora in wild type and inducible nitric oxide synthase (iNOS)-deficient mice (iNOS(-/-)) using the terminal restriction fragment length polymorphism analysis (T-RFLP) and 16S rDNA clone libraries. The amounts of bacterial DNA detected in fecal samples from both animal groups pretreated with antibiotics were extremely low as compared with untreated groups. We found that the profiles of enteric bacteria changed markedly after BDL. The bacterial composition is significantly different between not only wild type and iNOS(-/-) mice but also those before and after BDL, respectively. Among enteric bacteria examined, Lactobacillus murinus was found to increase markedly after BDL in rectum of both animal groups. However, Escherichia coli markedly increased after BDL in the iNOS(-/-) mice. These findings suggest that profiles of enteric flora change markedly in animals during obstructive jaundice by some mechanism that is affected by bile constituents and iNOS-derived NO.

No MeSH data available.


Related in: MedlinePlus

Histological examination of the expression of iNOS in the colon. At the indicated times after BDL, colon specimens were frozen, cut into thin sections, treated with anti-iNOS antibody and then stained with FITC-conjugated second antibody. Scale bar = 50 µm
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Figure 4: Histological examination of the expression of iNOS in the colon. At the indicated times after BDL, colon specimens were frozen, cut into thin sections, treated with anti-iNOS antibody and then stained with FITC-conjugated second antibody. Scale bar = 50 µm

Mentions: Colonic expression of iNOS markedly increased after BDL. As shown in Fig. 4 and 5, the expression of iNOS in the wild type mice increased after BDL, peaked on day 3, and decreased thereafter. Naturally, no iNOS was induced by BDL in the knockout mice.


Effect of Obstructive Jaundice and Nitric Oxide on the Profiles of Intestinal Bacterial Flora in Wild and iNOS Mice.

Hong JY, F Sato E, Nishikawa T, Hiramoto K, Inoue M - J Clin Biochem Nutr (2009)

Histological examination of the expression of iNOS in the colon. At the indicated times after BDL, colon specimens were frozen, cut into thin sections, treated with anti-iNOS antibody and then stained with FITC-conjugated second antibody. Scale bar = 50 µm
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2654472&req=5

Figure 4: Histological examination of the expression of iNOS in the colon. At the indicated times after BDL, colon specimens were frozen, cut into thin sections, treated with anti-iNOS antibody and then stained with FITC-conjugated second antibody. Scale bar = 50 µm
Mentions: Colonic expression of iNOS markedly increased after BDL. As shown in Fig. 4 and 5, the expression of iNOS in the wild type mice increased after BDL, peaked on day 3, and decreased thereafter. Naturally, no iNOS was induced by BDL in the knockout mice.

Bottom Line: The amounts of bacterial DNA detected in fecal samples from both animal groups pretreated with antibiotics were extremely low as compared with untreated groups.We found that the profiles of enteric bacteria changed markedly after BDL.Among enteric bacteria examined, Lactobacillus murinus was found to increase markedly after BDL in rectum of both animal groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Pathology, Osaka City University Medical School, 1-4-3 Asahimachi, Abeno, Osaka 545-8585, Japan.

ABSTRACT
We previously reported that the plasma level of endotoxin and colonic expression of IgA in the mouse increased with obstructive jaundice induced by bile duct ligation (BDL). To elucidate the mechanism of the BDL-induced increase, we analyzed the effect of BDL on intestinal flora in wild type and inducible nitric oxide synthase (iNOS)-deficient mice (iNOS(-/-)) using the terminal restriction fragment length polymorphism analysis (T-RFLP) and 16S rDNA clone libraries. The amounts of bacterial DNA detected in fecal samples from both animal groups pretreated with antibiotics were extremely low as compared with untreated groups. We found that the profiles of enteric bacteria changed markedly after BDL. The bacterial composition is significantly different between not only wild type and iNOS(-/-) mice but also those before and after BDL, respectively. Among enteric bacteria examined, Lactobacillus murinus was found to increase markedly after BDL in rectum of both animal groups. However, Escherichia coli markedly increased after BDL in the iNOS(-/-) mice. These findings suggest that profiles of enteric flora change markedly in animals during obstructive jaundice by some mechanism that is affected by bile constituents and iNOS-derived NO.

No MeSH data available.


Related in: MedlinePlus