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Beneficial Effects of Cocoa in Perivascular Mato Cells of Cerebral Arterioles in SHR-SP (Izm) Rats.

Mato T, Kamei M, Ito R, Sawano M, Inokuchi K, Nakata K, Yamaguchi A, Kouki T, Mitsuhashi U, Mato M - J Clin Biochem Nutr (2009)

Bottom Line: Vascular cells exhibited some signs of pathology.Signs of pathology in vascular cells were also decreased.Superoxides may impair Mato cells and vascular cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Emergency and Critical Care Medicine, Saitama Medical Center, Saitama Medical University, 1981 Kamoda, Kawagoe-shi, Saitama 350-8550, Japan.

ABSTRACT
As previously reported, the cerebral arterioles are surrounded by unique perivascular Mato cells. They contain many inclusion bodies rich in hydrolytic enzymes, and have strong uptake capacity. They are thus considered scavenger cells of vascular and neural tissues in steady-state. In this study, employing hypertensive SHR-SP (Izm) rats, the viability of Mato cells was investigated. In hypertensive rats, the capacity for uptake of horse radish peroxidase (HRP) and the activity of acid phosphatase (ACPase) of Mato cells were markedly reduced, and on electron-microscopic examination Mato cells were found to include heterogeneous contents and appeared electron-dense and degenerated. Vascular cells exhibited some signs of pathology. However, in hypertensive rats fed chow containing 0.25% cocoa, the uptake capacity and ACPase activity of Mato cells for HRP were enhanced, and on electron-microscopic examination Mato cells appeared healthy, with mitochondria with nearly normal profiles. Signs of pathology in vascular cells were also decreased. Superoxides may impair Mato cells and vascular cells.

No MeSH data available.


Related in: MedlinePlus

A to I are electron micrographs of Mato cells and cerebral arterioles.A (NF rat) and B (CF rat) are general view of cerebral arterioles, and at the outside of arterioles, Mato cells are displayed (M). The square of A is magnified in E, and that of B is in F. C to E are selected from NF rats, and F to H from CF rats.C: NF rats. Matos cell in this photograph are classified as the first type (type I) (M1) and second type (type II) (M2) in this paper. That is, two Mato cells are overlapped. The cell boundary is indicated by arrows.D: NF rats. This figure shows vascular cells (s) and a apart of extension of Mato cell (M). In the Mato cell of second type (type II), expanded endoplasmic reticula and vesicles are seen.M; Mato cell, E; endothelial cell, SM; smooth muscle cell, D; dense body, J; tight junction.E: NF rats. From the luminal surface of this figure, endothelium, smooth muscle cell and dark Mato cells (type III) are illustrated. Microglia (Mg) with large dense body abuts on Mato cell.E; endothelial cells, D; dense body, SM; smooth muscle cell, M; Mato cell, Mg; Microglia.F: CF rats. This figure shows endothelial cells, smooth muscle cells, and pale Mato cell. Mato cell is pale in cytoplasm and contain inclusions of various size. Some of them are vacuolized. The nucleus, endoplasmic reticula and mitochondria take normal profiles. E; endothelial cells, SM; smooth muscle cell, M; Mato cell, C; collagen fiber.G: CF rats. This figure shows vascular cells and some parts of extension of Mato cell. Endothelial cell protrudes into vascular lumen and contain mitochondria with normal profiles. In the outside of smooth muscle cells, extensions of Mato cells are displayed. It contains lysosomal inclusions with intense matrices. Around it, collagen fibers are seen (arrows).E; endothelial cells, SM; smooth muscle cell, M; Mato cell, C; collagen fiber, P; pinocytotic vesicle, J; tight junction.H: CF rat. Mato cell is pale and somewhat swollen, and contains several inclusions with or without vacuoles. Endoplasmic reticula gather in some part of cytoplasm. Pinocytosis is seen along the cytoplasmic membrane of endothelium and Mato cell (arrows). Mitochondria in the smooth muscle cells are stained intensely. Collagen fibers are scattered surrounding the Mato cells. E; endothelial cells, SM; smooth muscle cell, M; Mato cell, ER; Endoplasmic reticula, P; pinocytotic vesicle, C; collagen fiber.
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Figure 4: A to I are electron micrographs of Mato cells and cerebral arterioles.A (NF rat) and B (CF rat) are general view of cerebral arterioles, and at the outside of arterioles, Mato cells are displayed (M). The square of A is magnified in E, and that of B is in F. C to E are selected from NF rats, and F to H from CF rats.C: NF rats. Matos cell in this photograph are classified as the first type (type I) (M1) and second type (type II) (M2) in this paper. That is, two Mato cells are overlapped. The cell boundary is indicated by arrows.D: NF rats. This figure shows vascular cells (s) and a apart of extension of Mato cell (M). In the Mato cell of second type (type II), expanded endoplasmic reticula and vesicles are seen.M; Mato cell, E; endothelial cell, SM; smooth muscle cell, D; dense body, J; tight junction.E: NF rats. From the luminal surface of this figure, endothelium, smooth muscle cell and dark Mato cells (type III) are illustrated. Microglia (Mg) with large dense body abuts on Mato cell.E; endothelial cells, D; dense body, SM; smooth muscle cell, M; Mato cell, Mg; Microglia.F: CF rats. This figure shows endothelial cells, smooth muscle cells, and pale Mato cell. Mato cell is pale in cytoplasm and contain inclusions of various size. Some of them are vacuolized. The nucleus, endoplasmic reticula and mitochondria take normal profiles. E; endothelial cells, SM; smooth muscle cell, M; Mato cell, C; collagen fiber.G: CF rats. This figure shows vascular cells and some parts of extension of Mato cell. Endothelial cell protrudes into vascular lumen and contain mitochondria with normal profiles. In the outside of smooth muscle cells, extensions of Mato cells are displayed. It contains lysosomal inclusions with intense matrices. Around it, collagen fibers are seen (arrows).E; endothelial cells, SM; smooth muscle cell, M; Mato cell, C; collagen fiber, P; pinocytotic vesicle, J; tight junction.H: CF rat. Mato cell is pale and somewhat swollen, and contains several inclusions with or without vacuoles. Endoplasmic reticula gather in some part of cytoplasm. Pinocytosis is seen along the cytoplasmic membrane of endothelium and Mato cell (arrows). Mitochondria in the smooth muscle cells are stained intensely. Collagen fibers are scattered surrounding the Mato cells. E; endothelial cells, SM; smooth muscle cell, M; Mato cell, ER; Endoplasmic reticula, P; pinocytotic vesicle, C; collagen fiber.

Mentions: As demonstrated in Fig. 4A, C, E, the perivascular Mato cells were situated between the vascular wall and astrocytes or microglia (Fig. 4A, E). They were slender or cuboidal in shape, and contained oval or irregularly-shaped nuclei. However, the electron-density of Mato cells in NF rats varied from pale to dark. Therefore, 100 Mato cells were examined electron-microscopically and classified into three types, based on the intensity of the cytosol and properties of cytoplasmic organelles.


Beneficial Effects of Cocoa in Perivascular Mato Cells of Cerebral Arterioles in SHR-SP (Izm) Rats.

Mato T, Kamei M, Ito R, Sawano M, Inokuchi K, Nakata K, Yamaguchi A, Kouki T, Mitsuhashi U, Mato M - J Clin Biochem Nutr (2009)

A to I are electron micrographs of Mato cells and cerebral arterioles.A (NF rat) and B (CF rat) are general view of cerebral arterioles, and at the outside of arterioles, Mato cells are displayed (M). The square of A is magnified in E, and that of B is in F. C to E are selected from NF rats, and F to H from CF rats.C: NF rats. Matos cell in this photograph are classified as the first type (type I) (M1) and second type (type II) (M2) in this paper. That is, two Mato cells are overlapped. The cell boundary is indicated by arrows.D: NF rats. This figure shows vascular cells (s) and a apart of extension of Mato cell (M). In the Mato cell of second type (type II), expanded endoplasmic reticula and vesicles are seen.M; Mato cell, E; endothelial cell, SM; smooth muscle cell, D; dense body, J; tight junction.E: NF rats. From the luminal surface of this figure, endothelium, smooth muscle cell and dark Mato cells (type III) are illustrated. Microglia (Mg) with large dense body abuts on Mato cell.E; endothelial cells, D; dense body, SM; smooth muscle cell, M; Mato cell, Mg; Microglia.F: CF rats. This figure shows endothelial cells, smooth muscle cells, and pale Mato cell. Mato cell is pale in cytoplasm and contain inclusions of various size. Some of them are vacuolized. The nucleus, endoplasmic reticula and mitochondria take normal profiles. E; endothelial cells, SM; smooth muscle cell, M; Mato cell, C; collagen fiber.G: CF rats. This figure shows vascular cells and some parts of extension of Mato cell. Endothelial cell protrudes into vascular lumen and contain mitochondria with normal profiles. In the outside of smooth muscle cells, extensions of Mato cells are displayed. It contains lysosomal inclusions with intense matrices. Around it, collagen fibers are seen (arrows).E; endothelial cells, SM; smooth muscle cell, M; Mato cell, C; collagen fiber, P; pinocytotic vesicle, J; tight junction.H: CF rat. Mato cell is pale and somewhat swollen, and contains several inclusions with or without vacuoles. Endoplasmic reticula gather in some part of cytoplasm. Pinocytosis is seen along the cytoplasmic membrane of endothelium and Mato cell (arrows). Mitochondria in the smooth muscle cells are stained intensely. Collagen fibers are scattered surrounding the Mato cells. E; endothelial cells, SM; smooth muscle cell, M; Mato cell, ER; Endoplasmic reticula, P; pinocytotic vesicle, C; collagen fiber.
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Related In: Results  -  Collection

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Figure 4: A to I are electron micrographs of Mato cells and cerebral arterioles.A (NF rat) and B (CF rat) are general view of cerebral arterioles, and at the outside of arterioles, Mato cells are displayed (M). The square of A is magnified in E, and that of B is in F. C to E are selected from NF rats, and F to H from CF rats.C: NF rats. Matos cell in this photograph are classified as the first type (type I) (M1) and second type (type II) (M2) in this paper. That is, two Mato cells are overlapped. The cell boundary is indicated by arrows.D: NF rats. This figure shows vascular cells (s) and a apart of extension of Mato cell (M). In the Mato cell of second type (type II), expanded endoplasmic reticula and vesicles are seen.M; Mato cell, E; endothelial cell, SM; smooth muscle cell, D; dense body, J; tight junction.E: NF rats. From the luminal surface of this figure, endothelium, smooth muscle cell and dark Mato cells (type III) are illustrated. Microglia (Mg) with large dense body abuts on Mato cell.E; endothelial cells, D; dense body, SM; smooth muscle cell, M; Mato cell, Mg; Microglia.F: CF rats. This figure shows endothelial cells, smooth muscle cells, and pale Mato cell. Mato cell is pale in cytoplasm and contain inclusions of various size. Some of them are vacuolized. The nucleus, endoplasmic reticula and mitochondria take normal profiles. E; endothelial cells, SM; smooth muscle cell, M; Mato cell, C; collagen fiber.G: CF rats. This figure shows vascular cells and some parts of extension of Mato cell. Endothelial cell protrudes into vascular lumen and contain mitochondria with normal profiles. In the outside of smooth muscle cells, extensions of Mato cells are displayed. It contains lysosomal inclusions with intense matrices. Around it, collagen fibers are seen (arrows).E; endothelial cells, SM; smooth muscle cell, M; Mato cell, C; collagen fiber, P; pinocytotic vesicle, J; tight junction.H: CF rat. Mato cell is pale and somewhat swollen, and contains several inclusions with or without vacuoles. Endoplasmic reticula gather in some part of cytoplasm. Pinocytosis is seen along the cytoplasmic membrane of endothelium and Mato cell (arrows). Mitochondria in the smooth muscle cells are stained intensely. Collagen fibers are scattered surrounding the Mato cells. E; endothelial cells, SM; smooth muscle cell, M; Mato cell, ER; Endoplasmic reticula, P; pinocytotic vesicle, C; collagen fiber.
Mentions: As demonstrated in Fig. 4A, C, E, the perivascular Mato cells were situated between the vascular wall and astrocytes or microglia (Fig. 4A, E). They were slender or cuboidal in shape, and contained oval or irregularly-shaped nuclei. However, the electron-density of Mato cells in NF rats varied from pale to dark. Therefore, 100 Mato cells were examined electron-microscopically and classified into three types, based on the intensity of the cytosol and properties of cytoplasmic organelles.

Bottom Line: Vascular cells exhibited some signs of pathology.Signs of pathology in vascular cells were also decreased.Superoxides may impair Mato cells and vascular cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Emergency and Critical Care Medicine, Saitama Medical Center, Saitama Medical University, 1981 Kamoda, Kawagoe-shi, Saitama 350-8550, Japan.

ABSTRACT
As previously reported, the cerebral arterioles are surrounded by unique perivascular Mato cells. They contain many inclusion bodies rich in hydrolytic enzymes, and have strong uptake capacity. They are thus considered scavenger cells of vascular and neural tissues in steady-state. In this study, employing hypertensive SHR-SP (Izm) rats, the viability of Mato cells was investigated. In hypertensive rats, the capacity for uptake of horse radish peroxidase (HRP) and the activity of acid phosphatase (ACPase) of Mato cells were markedly reduced, and on electron-microscopic examination Mato cells were found to include heterogeneous contents and appeared electron-dense and degenerated. Vascular cells exhibited some signs of pathology. However, in hypertensive rats fed chow containing 0.25% cocoa, the uptake capacity and ACPase activity of Mato cells for HRP were enhanced, and on electron-microscopic examination Mato cells appeared healthy, with mitochondria with nearly normal profiles. Signs of pathology in vascular cells were also decreased. Superoxides may impair Mato cells and vascular cells.

No MeSH data available.


Related in: MedlinePlus