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Biocompatibility of Poly-epsilon-caprolactone-hydroxyapatite composite on mouse bone marrow-derived osteoblasts and endothelial cells.

Yu H, Wooley PH, Yang SY - J Orthop Surg Res (2009)

Bottom Line: The results indicated that HA led to a positive stimulation of osteoblasts viability and ALP activity, while HA showed less influence on endothelial cells viability.Supplement of HA into PCL improved biocompatible for bone marrow-derived osteoblasts and endothelial cells.The PCL-HA composite integrating with two types of cells may provide a useful system for tissue-engineered bone grafts with vascularization.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biomedical Engineering, Wayne State University, Detroit, Michigan, USA. shang-you.yang@wichita.edu.

ABSTRACT

Background: Tissue-engineered bone may be developed by seeding the cells capable of both osteogenesis and vascularization on biocompatible composite scaffolds. The current study investigated the performance of mice bone marrow-derived osteogenic cells and endothelial cells as seeded on hydroxyapatite (HA) and poly-epsilon-caprolactone (PCL) composite scaffolds.

Methods: Mononuclear cells were induced to osteoblasts and endothelial cells respectively, which were defined by the expression of osteocalcin, alkaline phosphatase (ALP), and deposits of calcium-containing crystal for osteoblasts, or by the expression of vascular endothelial growth factor receptor-2 (VEGFR-2) and von Willebrand factor (vWF), and the formation of a capillary network in Matrigel for endothelial cells. Both types of cell were seeded respectively on PCL-HA scaffolds at HA to PCL weight ratio of 1:1, 1:4, or 0:1 and were evaluated using scanning electron microscopy, ALP activity (of osteoblasts) and nitric oxide production (of endothelial cells) plus the assessment of cell viability.

Results: The results indicated that HA led to a positive stimulation of osteoblasts viability and ALP activity, while HA showed less influence on endothelial cells viability. An elevated nitric oxide production of endothelial cells was observed in HA-containing group.

Conclusion: Supplement of HA into PCL improved biocompatible for bone marrow-derived osteoblasts and endothelial cells. The PCL-HA composite integrating with two types of cells may provide a useful system for tissue-engineered bone grafts with vascularization.

No MeSH data available.


Related in: MedlinePlus

Cells morphology on the composite scaffolds (HA: PCL = 1:1). Panel (A) showed osteoblasts proliferating on PCL-HA scaffolds (400×). Panel (B) was an enlargement of Panel (A) showing the details of the attached osteoblasts with meshwork of extracellular matrix (arrows), and cellular projection (1500×). Panels (C) and (D) revealed the growth of endothelial cells on a PCL-HA scaffold. The Panel (C) pictured ECs proliferating and forming a cell sheet (600×), and Panel (D) magnified attached ECs to detail their cobblestone shape and cellular extensions (4000×).
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Figure 5: Cells morphology on the composite scaffolds (HA: PCL = 1:1). Panel (A) showed osteoblasts proliferating on PCL-HA scaffolds (400×). Panel (B) was an enlargement of Panel (A) showing the details of the attached osteoblasts with meshwork of extracellular matrix (arrows), and cellular projection (1500×). Panels (C) and (D) revealed the growth of endothelial cells on a PCL-HA scaffold. The Panel (C) pictured ECs proliferating and forming a cell sheet (600×), and Panel (D) magnified attached ECs to detail their cobblestone shape and cellular extensions (4000×).

Mentions: The specific cell morphology of either osteoblasts or endothelial cells displayed rarely difference response to various groups of PCL-HA scaffold. The continuous culture of osteoblasts on HA-containing scaffolds for 7 days revealed that cells retained their spindle morphology (Figure 5A) similar to the osteoblasts grown on tissue culture flasks. Extracellular matrix (collagen-like fibers) was clearly present at intercellular regions, where cellular projections were evident (Figure 5B). For the cultures of PCL-HA scaffolds with bone marrow-derived endothelial cells, the cells completely covered the surface of the scaffolds at 7 days (Figure 5C). The spreading and paving endothelial cells remained as typically cobblestone like shapes with high cell-to-cell contact (Figure 5D). Cellular extensions on the cells surface were also detected.


Biocompatibility of Poly-epsilon-caprolactone-hydroxyapatite composite on mouse bone marrow-derived osteoblasts and endothelial cells.

Yu H, Wooley PH, Yang SY - J Orthop Surg Res (2009)

Cells morphology on the composite scaffolds (HA: PCL = 1:1). Panel (A) showed osteoblasts proliferating on PCL-HA scaffolds (400×). Panel (B) was an enlargement of Panel (A) showing the details of the attached osteoblasts with meshwork of extracellular matrix (arrows), and cellular projection (1500×). Panels (C) and (D) revealed the growth of endothelial cells on a PCL-HA scaffold. The Panel (C) pictured ECs proliferating and forming a cell sheet (600×), and Panel (D) magnified attached ECs to detail their cobblestone shape and cellular extensions (4000×).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2654429&req=5

Figure 5: Cells morphology on the composite scaffolds (HA: PCL = 1:1). Panel (A) showed osteoblasts proliferating on PCL-HA scaffolds (400×). Panel (B) was an enlargement of Panel (A) showing the details of the attached osteoblasts with meshwork of extracellular matrix (arrows), and cellular projection (1500×). Panels (C) and (D) revealed the growth of endothelial cells on a PCL-HA scaffold. The Panel (C) pictured ECs proliferating and forming a cell sheet (600×), and Panel (D) magnified attached ECs to detail their cobblestone shape and cellular extensions (4000×).
Mentions: The specific cell morphology of either osteoblasts or endothelial cells displayed rarely difference response to various groups of PCL-HA scaffold. The continuous culture of osteoblasts on HA-containing scaffolds for 7 days revealed that cells retained their spindle morphology (Figure 5A) similar to the osteoblasts grown on tissue culture flasks. Extracellular matrix (collagen-like fibers) was clearly present at intercellular regions, where cellular projections were evident (Figure 5B). For the cultures of PCL-HA scaffolds with bone marrow-derived endothelial cells, the cells completely covered the surface of the scaffolds at 7 days (Figure 5C). The spreading and paving endothelial cells remained as typically cobblestone like shapes with high cell-to-cell contact (Figure 5D). Cellular extensions on the cells surface were also detected.

Bottom Line: The results indicated that HA led to a positive stimulation of osteoblasts viability and ALP activity, while HA showed less influence on endothelial cells viability.Supplement of HA into PCL improved biocompatible for bone marrow-derived osteoblasts and endothelial cells.The PCL-HA composite integrating with two types of cells may provide a useful system for tissue-engineered bone grafts with vascularization.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biomedical Engineering, Wayne State University, Detroit, Michigan, USA. shang-you.yang@wichita.edu.

ABSTRACT

Background: Tissue-engineered bone may be developed by seeding the cells capable of both osteogenesis and vascularization on biocompatible composite scaffolds. The current study investigated the performance of mice bone marrow-derived osteogenic cells and endothelial cells as seeded on hydroxyapatite (HA) and poly-epsilon-caprolactone (PCL) composite scaffolds.

Methods: Mononuclear cells were induced to osteoblasts and endothelial cells respectively, which were defined by the expression of osteocalcin, alkaline phosphatase (ALP), and deposits of calcium-containing crystal for osteoblasts, or by the expression of vascular endothelial growth factor receptor-2 (VEGFR-2) and von Willebrand factor (vWF), and the formation of a capillary network in Matrigel for endothelial cells. Both types of cell were seeded respectively on PCL-HA scaffolds at HA to PCL weight ratio of 1:1, 1:4, or 0:1 and were evaluated using scanning electron microscopy, ALP activity (of osteoblasts) and nitric oxide production (of endothelial cells) plus the assessment of cell viability.

Results: The results indicated that HA led to a positive stimulation of osteoblasts viability and ALP activity, while HA showed less influence on endothelial cells viability. An elevated nitric oxide production of endothelial cells was observed in HA-containing group.

Conclusion: Supplement of HA into PCL improved biocompatible for bone marrow-derived osteoblasts and endothelial cells. The PCL-HA composite integrating with two types of cells may provide a useful system for tissue-engineered bone grafts with vascularization.

No MeSH data available.


Related in: MedlinePlus