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Biocompatibility of Poly-epsilon-caprolactone-hydroxyapatite composite on mouse bone marrow-derived osteoblasts and endothelial cells.

Yu H, Wooley PH, Yang SY - J Orthop Surg Res (2009)

Bottom Line: The results indicated that HA led to a positive stimulation of osteoblasts viability and ALP activity, while HA showed less influence on endothelial cells viability.Supplement of HA into PCL improved biocompatible for bone marrow-derived osteoblasts and endothelial cells.The PCL-HA composite integrating with two types of cells may provide a useful system for tissue-engineered bone grafts with vascularization.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biomedical Engineering, Wayne State University, Detroit, Michigan, USA. shang-you.yang@wichita.edu.

ABSTRACT

Background: Tissue-engineered bone may be developed by seeding the cells capable of both osteogenesis and vascularization on biocompatible composite scaffolds. The current study investigated the performance of mice bone marrow-derived osteogenic cells and endothelial cells as seeded on hydroxyapatite (HA) and poly-epsilon-caprolactone (PCL) composite scaffolds.

Methods: Mononuclear cells were induced to osteoblasts and endothelial cells respectively, which were defined by the expression of osteocalcin, alkaline phosphatase (ALP), and deposits of calcium-containing crystal for osteoblasts, or by the expression of vascular endothelial growth factor receptor-2 (VEGFR-2) and von Willebrand factor (vWF), and the formation of a capillary network in Matrigel for endothelial cells. Both types of cell were seeded respectively on PCL-HA scaffolds at HA to PCL weight ratio of 1:1, 1:4, or 0:1 and were evaluated using scanning electron microscopy, ALP activity (of osteoblasts) and nitric oxide production (of endothelial cells) plus the assessment of cell viability.

Results: The results indicated that HA led to a positive stimulation of osteoblasts viability and ALP activity, while HA showed less influence on endothelial cells viability. An elevated nitric oxide production of endothelial cells was observed in HA-containing group.

Conclusion: Supplement of HA into PCL improved biocompatible for bone marrow-derived osteoblasts and endothelial cells. The PCL-HA composite integrating with two types of cells may provide a useful system for tissue-engineered bone grafts with vascularization.

No MeSH data available.


Related in: MedlinePlus

Microstructure of scaffolds exhibited by SEM. The HA were embedded in PCL, or exposed on the surface. Apparently the roughness of pore-wall surfaces increased with increasing the HA ratio. Panel (A) (800×) was an example of HA-free PCL scaffolds. Panel (B) (1000×) showed composite with low HA ratio, HA: PCL (w/w) = 1:4; while Panel (C) (500×) revealed a sample with high HA concentration, HA: PCL (w/w) = 1:1. The protruded components were confirmed as the HA by EDX (Panel D). The peaks of calcium and phosphorus were prominent and quantified the atomic ratio (Ca: P = 1.58).
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Figure 4: Microstructure of scaffolds exhibited by SEM. The HA were embedded in PCL, or exposed on the surface. Apparently the roughness of pore-wall surfaces increased with increasing the HA ratio. Panel (A) (800×) was an example of HA-free PCL scaffolds. Panel (B) (1000×) showed composite with low HA ratio, HA: PCL (w/w) = 1:4; while Panel (C) (500×) revealed a sample with high HA concentration, HA: PCL (w/w) = 1:1. The protruded components were confirmed as the HA by EDX (Panel D). The peaks of calcium and phosphorus were prominent and quantified the atomic ratio (Ca: P = 1.58).

Mentions: PCL scaffolds incorporated with or without HA were fabricated with controlled porosity (70% ~ 75%) and pore sizes. Interconnected pore morphologies were present in all scaffolds, resulting in the high porosity of the scaffolds. Different microtopographies of scaffolds were revealed by scanning electron microscopy (SEM) (Figure 4). The roughness of pore wall appeared dependent on the ratio of HA to PCL. High HA concentration led to extensive protrusions of HA particles and rough surfaces (Figure 4C), while an almost smooth pore wall was achieved in the PCL scaffold without HA incorporation (Figure 4A). EDX analysis indicated atomic ratio of calcium to phosphorus (Ca: P = 1.58) on both low HA ratio (HA: PCL = 1:4) and high HA ratio (HA: PCL = 1:1) composite scaffolds, which is comparable to a natural hydroxyapatite (Figure 4D).


Biocompatibility of Poly-epsilon-caprolactone-hydroxyapatite composite on mouse bone marrow-derived osteoblasts and endothelial cells.

Yu H, Wooley PH, Yang SY - J Orthop Surg Res (2009)

Microstructure of scaffolds exhibited by SEM. The HA were embedded in PCL, or exposed on the surface. Apparently the roughness of pore-wall surfaces increased with increasing the HA ratio. Panel (A) (800×) was an example of HA-free PCL scaffolds. Panel (B) (1000×) showed composite with low HA ratio, HA: PCL (w/w) = 1:4; while Panel (C) (500×) revealed a sample with high HA concentration, HA: PCL (w/w) = 1:1. The protruded components were confirmed as the HA by EDX (Panel D). The peaks of calcium and phosphorus were prominent and quantified the atomic ratio (Ca: P = 1.58).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2654429&req=5

Figure 4: Microstructure of scaffolds exhibited by SEM. The HA were embedded in PCL, or exposed on the surface. Apparently the roughness of pore-wall surfaces increased with increasing the HA ratio. Panel (A) (800×) was an example of HA-free PCL scaffolds. Panel (B) (1000×) showed composite with low HA ratio, HA: PCL (w/w) = 1:4; while Panel (C) (500×) revealed a sample with high HA concentration, HA: PCL (w/w) = 1:1. The protruded components were confirmed as the HA by EDX (Panel D). The peaks of calcium and phosphorus were prominent and quantified the atomic ratio (Ca: P = 1.58).
Mentions: PCL scaffolds incorporated with or without HA were fabricated with controlled porosity (70% ~ 75%) and pore sizes. Interconnected pore morphologies were present in all scaffolds, resulting in the high porosity of the scaffolds. Different microtopographies of scaffolds were revealed by scanning electron microscopy (SEM) (Figure 4). The roughness of pore wall appeared dependent on the ratio of HA to PCL. High HA concentration led to extensive protrusions of HA particles and rough surfaces (Figure 4C), while an almost smooth pore wall was achieved in the PCL scaffold without HA incorporation (Figure 4A). EDX analysis indicated atomic ratio of calcium to phosphorus (Ca: P = 1.58) on both low HA ratio (HA: PCL = 1:4) and high HA ratio (HA: PCL = 1:1) composite scaffolds, which is comparable to a natural hydroxyapatite (Figure 4D).

Bottom Line: The results indicated that HA led to a positive stimulation of osteoblasts viability and ALP activity, while HA showed less influence on endothelial cells viability.Supplement of HA into PCL improved biocompatible for bone marrow-derived osteoblasts and endothelial cells.The PCL-HA composite integrating with two types of cells may provide a useful system for tissue-engineered bone grafts with vascularization.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biomedical Engineering, Wayne State University, Detroit, Michigan, USA. shang-you.yang@wichita.edu.

ABSTRACT

Background: Tissue-engineered bone may be developed by seeding the cells capable of both osteogenesis and vascularization on biocompatible composite scaffolds. The current study investigated the performance of mice bone marrow-derived osteogenic cells and endothelial cells as seeded on hydroxyapatite (HA) and poly-epsilon-caprolactone (PCL) composite scaffolds.

Methods: Mononuclear cells were induced to osteoblasts and endothelial cells respectively, which were defined by the expression of osteocalcin, alkaline phosphatase (ALP), and deposits of calcium-containing crystal for osteoblasts, or by the expression of vascular endothelial growth factor receptor-2 (VEGFR-2) and von Willebrand factor (vWF), and the formation of a capillary network in Matrigel for endothelial cells. Both types of cell were seeded respectively on PCL-HA scaffolds at HA to PCL weight ratio of 1:1, 1:4, or 0:1 and were evaluated using scanning electron microscopy, ALP activity (of osteoblasts) and nitric oxide production (of endothelial cells) plus the assessment of cell viability.

Results: The results indicated that HA led to a positive stimulation of osteoblasts viability and ALP activity, while HA showed less influence on endothelial cells viability. An elevated nitric oxide production of endothelial cells was observed in HA-containing group.

Conclusion: Supplement of HA into PCL improved biocompatible for bone marrow-derived osteoblasts and endothelial cells. The PCL-HA composite integrating with two types of cells may provide a useful system for tissue-engineered bone grafts with vascularization.

No MeSH data available.


Related in: MedlinePlus