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Unfolding chromatin nets

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During an infection or inflammation, dying neutrophils spill their DNA to form pathogen-trapping NETs (neutrophil extracellular traps)... Wang et al. tested whether another histone adjustment—replacing the positively charged amino acid arginine with the electrically neutral amino acid citrulline—helps neutrophil DNA loosen up during NET formation... The enzyme that catalyzes this exchange is PAD4, and blocking it hinders NET formation, the team found... The researchers also showed that citrulline swaps catalyzed by PAD4 prompt cells to relax their heterochromatin, the tightly wrapped form of chromatin... One way that cells cinch up their DNA is through the linker histone, which compacts chromatin by connecting neighboring nucleosomes... Wang et al.'s results suggest that citrulline replacement prevents the histone from maintaining this tight link... Overall, the work reveals that PAD4 spurs the chromatin decondensation necessary to create neutrophil nets... An open question is whether other cell types rely on PAD4 when they need to unwind their DNA... For example, the protein's chromatin loosening prowess might prove handy during programmed cell death.

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A neutrophil extracellular trap (red) is ready to snare passing microbes.
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fig1: A neutrophil extracellular trap (red) is ready to snare passing microbes.


Unfolding chromatin nets
A neutrophil extracellular trap (red) is ready to snare passing microbes.
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Related In: Results  -  Collection

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fig1: A neutrophil extracellular trap (red) is ready to snare passing microbes.

View Article: PubMed Central

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

During an infection or inflammation, dying neutrophils spill their DNA to form pathogen-trapping NETs (neutrophil extracellular traps)... Wang et al. tested whether another histone adjustment—replacing the positively charged amino acid arginine with the electrically neutral amino acid citrulline—helps neutrophil DNA loosen up during NET formation... The enzyme that catalyzes this exchange is PAD4, and blocking it hinders NET formation, the team found... The researchers also showed that citrulline swaps catalyzed by PAD4 prompt cells to relax their heterochromatin, the tightly wrapped form of chromatin... One way that cells cinch up their DNA is through the linker histone, which compacts chromatin by connecting neighboring nucleosomes... Wang et al.'s results suggest that citrulline replacement prevents the histone from maintaining this tight link... Overall, the work reveals that PAD4 spurs the chromatin decondensation necessary to create neutrophil nets... An open question is whether other cell types rely on PAD4 when they need to unwind their DNA... For example, the protein's chromatin loosening prowess might prove handy during programmed cell death.

No MeSH data available.


Related in: MedlinePlus