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Serotonin increases cilia-driven particle transport via an acetylcholine-independent pathway in the mouse trachea.

König P, Krain B, Krasteva G, Kummer W - PLoS ONE (2009)

Bottom Line: Classical neuromediators such as noradrenalin and acetylcholine increase ciliary beat frequency and thus cilia-driven transport.The increase in particle transport speed was totally prevented by methysergide (100 microM).Blockade of muscarinic receptors by atropine (1 microM) did not reduce the effect of serotonin, although it was effective in preventing the increase in particle transport speed mediated by muscarine (100 microM).

View Article: PubMed Central - PubMed

Affiliation: Institut für Anatomie, Zentrum für medizinische Struktur- und Zellbiologie, Universität zu Lübeck, Lübeck, Germany. koenig@anat.uni-luebeck.de

ABSTRACT

Background: Mucociliary clearance in the airways is driven by the coordinated beating of ciliated cells. Classical neuromediators such as noradrenalin and acetylcholine increase ciliary beat frequency and thus cilia-driven transport. Despite the fact that the neuromediator serotonin is ciliostimulatory in invertebrates and has been implied in releasing acetylcholine from the airway epithelium, its role in regulating cilia function in vertebrate airways is not established.

Methodology/principal findings: We examined the effects of serotonin on ciliary beat frequency and cilia-driven particle transport in the acutely excised submerged mouse trachea and determined the sources of serotonin in this tissue by immunohistochemistry. Serotonin (100 microM) increased cilary beat frequency (8.9+/-1.2 Hz to 17.0+/-2.7 Hz) and particle transport speed (38.9+/-4.6 microm/s to 83.4+/-8.3 microm/s) to an extent that was comparable to a supramaximal dose of ATP. The increase in particle transport speed was totally prevented by methysergide (100 microM). Blockade of muscarinic receptors by atropine (1 microM) did not reduce the effect of serotonin, although it was effective in preventing the increase in particle transport speed mediated by muscarine (100 microM). Immunohistochemistry demonstrated serotonin in mast cells pointing towards mast cells and platelets as possible endogenous sources of serotonin.

Conclusions/significance: These results indicate that serotonin is a likely endogenous mediator that can increase cilia-driven transport independent from acetylcholine during activation of mast cells and platelets.

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Scanning electron microscopy of the tracheal epithelium after the experiment.A. No continuous mucus layer can be detected on the epithelial surface after the experiments. B. Occasionally, small amounts of mucus are found on the epithelium . Small mucus-particle aggregates were also occasionally observed during measurements of particle transport speed (compare Movie S2). Bars = 10 µm.
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pone-0004938-g002: Scanning electron microscopy of the tracheal epithelium after the experiment.A. No continuous mucus layer can be detected on the epithelial surface after the experiments. B. Occasionally, small amounts of mucus are found on the epithelium . Small mucus-particle aggregates were also occasionally observed during measurements of particle transport speed (compare Movie S2). Bars = 10 µm.

Mentions: In the submerged trachea, particles were transported cranially by coordinated beating of ciliated cells. Transport was not dependent on the size of the particles (see Movie S1) and did not involve a continuous mucus layer as verified by examining tracheae after the experiment using scanning electron microscopy (Figure 2A). Occasionally, small amounts of mucus were present and entrapped particles which were dragged along the epithelial surface (see Movie S2). Small areas that were covered with mucus were occasionally detected by scanning electron microscopy after the experiment (Figure 2B).


Serotonin increases cilia-driven particle transport via an acetylcholine-independent pathway in the mouse trachea.

König P, Krain B, Krasteva G, Kummer W - PLoS ONE (2009)

Scanning electron microscopy of the tracheal epithelium after the experiment.A. No continuous mucus layer can be detected on the epithelial surface after the experiments. B. Occasionally, small amounts of mucus are found on the epithelium . Small mucus-particle aggregates were also occasionally observed during measurements of particle transport speed (compare Movie S2). Bars = 10 µm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2654158&req=5

pone-0004938-g002: Scanning electron microscopy of the tracheal epithelium after the experiment.A. No continuous mucus layer can be detected on the epithelial surface after the experiments. B. Occasionally, small amounts of mucus are found on the epithelium . Small mucus-particle aggregates were also occasionally observed during measurements of particle transport speed (compare Movie S2). Bars = 10 µm.
Mentions: In the submerged trachea, particles were transported cranially by coordinated beating of ciliated cells. Transport was not dependent on the size of the particles (see Movie S1) and did not involve a continuous mucus layer as verified by examining tracheae after the experiment using scanning electron microscopy (Figure 2A). Occasionally, small amounts of mucus were present and entrapped particles which were dragged along the epithelial surface (see Movie S2). Small areas that were covered with mucus were occasionally detected by scanning electron microscopy after the experiment (Figure 2B).

Bottom Line: Classical neuromediators such as noradrenalin and acetylcholine increase ciliary beat frequency and thus cilia-driven transport.The increase in particle transport speed was totally prevented by methysergide (100 microM).Blockade of muscarinic receptors by atropine (1 microM) did not reduce the effect of serotonin, although it was effective in preventing the increase in particle transport speed mediated by muscarine (100 microM).

View Article: PubMed Central - PubMed

Affiliation: Institut für Anatomie, Zentrum für medizinische Struktur- und Zellbiologie, Universität zu Lübeck, Lübeck, Germany. koenig@anat.uni-luebeck.de

ABSTRACT

Background: Mucociliary clearance in the airways is driven by the coordinated beating of ciliated cells. Classical neuromediators such as noradrenalin and acetylcholine increase ciliary beat frequency and thus cilia-driven transport. Despite the fact that the neuromediator serotonin is ciliostimulatory in invertebrates and has been implied in releasing acetylcholine from the airway epithelium, its role in regulating cilia function in vertebrate airways is not established.

Methodology/principal findings: We examined the effects of serotonin on ciliary beat frequency and cilia-driven particle transport in the acutely excised submerged mouse trachea and determined the sources of serotonin in this tissue by immunohistochemistry. Serotonin (100 microM) increased cilary beat frequency (8.9+/-1.2 Hz to 17.0+/-2.7 Hz) and particle transport speed (38.9+/-4.6 microm/s to 83.4+/-8.3 microm/s) to an extent that was comparable to a supramaximal dose of ATP. The increase in particle transport speed was totally prevented by methysergide (100 microM). Blockade of muscarinic receptors by atropine (1 microM) did not reduce the effect of serotonin, although it was effective in preventing the increase in particle transport speed mediated by muscarine (100 microM). Immunohistochemistry demonstrated serotonin in mast cells pointing towards mast cells and platelets as possible endogenous sources of serotonin.

Conclusions/significance: These results indicate that serotonin is a likely endogenous mediator that can increase cilia-driven transport independent from acetylcholine during activation of mast cells and platelets.

Show MeSH
Related in: MedlinePlus