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Expression of 5S rDNA in the oocytes of water frogs.

Czarniewska E, Plewa R - BMC Res Notes (2009)

Bottom Line: We report the expression pattern of 5S rDNA in the eggs of water frogs Rana lessonae, Rana ridibunda and Rana esculenta using the quantitative real-time PCR.This kind of research had never been performed before. 5S rDNA relative expression of the Rana ridibunda oocytes is approximately six times higher in comparison to the Rana lessonae oocytes.The oocytes of the investigated Rana esculenta frogs, in respect of 5S rDNA relative expression ratio, were very similar to the Rana ridibunda oocytes.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Animal Physiology and Development, Institute of Experimental Biology, Adam Mickiewicz University, Umultowska Str, 89, 61-614 Poznañ, Poland. czarniew@amu.edu.pl

ABSTRACT

Background: We report the expression pattern of 5S rDNA in the eggs of water frogs Rana lessonae, Rana ridibunda and Rana esculenta using the quantitative real-time PCR. This kind of research had never been performed before.

Results: 5S rDNA relative expression of the Rana ridibunda oocytes is approximately six times higher in comparison to the Rana lessonae oocytes. The oocytes of the investigated Rana esculenta frogs, in respect of 5S rDNA relative expression ratio, were very similar to the Rana ridibunda oocytes.

Conclusion: We suggest the possibility of using 5S rDNA as the internal control gene, in the studies of relative mRNA quantitative assays in water frog oocytes, because of its characteristic specific expression pattern in the Rana lessonae, Rana ridibunda and Rana esculenta oocytes.

No MeSH data available.


Relative expression of 5S rDNA in LL, RR and RL oocytes. The error bars represent SEM (* statistically significant); p < 0.05. LL – R. lessonae, RR – R. ridibunda, RL – R. esculenta.
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Figure 3: Relative expression of 5S rDNA in LL, RR and RL oocytes. The error bars represent SEM (* statistically significant); p < 0.05. LL – R. lessonae, RR – R. ridibunda, RL – R. esculenta.

Mentions: The expression levels of 5S rDNA in the oocytes of the two Mendelian species are significantly different, approximately six times higher in RR oocytes than in LL oocytes. 5S rDNA expression in oocytes of RL frogs investigated, was similar to that of RR oocytes and much higher than that of LL oocytes (Fig. 3).


Expression of 5S rDNA in the oocytes of water frogs.

Czarniewska E, Plewa R - BMC Res Notes (2009)

Relative expression of 5S rDNA in LL, RR and RL oocytes. The error bars represent SEM (* statistically significant); p < 0.05. LL – R. lessonae, RR – R. ridibunda, RL – R. esculenta.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2651904&req=5

Figure 3: Relative expression of 5S rDNA in LL, RR and RL oocytes. The error bars represent SEM (* statistically significant); p < 0.05. LL – R. lessonae, RR – R. ridibunda, RL – R. esculenta.
Mentions: The expression levels of 5S rDNA in the oocytes of the two Mendelian species are significantly different, approximately six times higher in RR oocytes than in LL oocytes. 5S rDNA expression in oocytes of RL frogs investigated, was similar to that of RR oocytes and much higher than that of LL oocytes (Fig. 3).

Bottom Line: We report the expression pattern of 5S rDNA in the eggs of water frogs Rana lessonae, Rana ridibunda and Rana esculenta using the quantitative real-time PCR.This kind of research had never been performed before. 5S rDNA relative expression of the Rana ridibunda oocytes is approximately six times higher in comparison to the Rana lessonae oocytes.The oocytes of the investigated Rana esculenta frogs, in respect of 5S rDNA relative expression ratio, were very similar to the Rana ridibunda oocytes.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Animal Physiology and Development, Institute of Experimental Biology, Adam Mickiewicz University, Umultowska Str, 89, 61-614 Poznañ, Poland. czarniew@amu.edu.pl

ABSTRACT

Background: We report the expression pattern of 5S rDNA in the eggs of water frogs Rana lessonae, Rana ridibunda and Rana esculenta using the quantitative real-time PCR. This kind of research had never been performed before.

Results: 5S rDNA relative expression of the Rana ridibunda oocytes is approximately six times higher in comparison to the Rana lessonae oocytes. The oocytes of the investigated Rana esculenta frogs, in respect of 5S rDNA relative expression ratio, were very similar to the Rana ridibunda oocytes.

Conclusion: We suggest the possibility of using 5S rDNA as the internal control gene, in the studies of relative mRNA quantitative assays in water frog oocytes, because of its characteristic specific expression pattern in the Rana lessonae, Rana ridibunda and Rana esculenta oocytes.

No MeSH data available.