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Expression of 5S rDNA in the oocytes of water frogs.

Czarniewska E, Plewa R - BMC Res Notes (2009)

Bottom Line: We report the expression pattern of 5S rDNA in the eggs of water frogs Rana lessonae, Rana ridibunda and Rana esculenta using the quantitative real-time PCR.This kind of research had never been performed before. 5S rDNA relative expression of the Rana ridibunda oocytes is approximately six times higher in comparison to the Rana lessonae oocytes.The oocytes of the investigated Rana esculenta frogs, in respect of 5S rDNA relative expression ratio, were very similar to the Rana ridibunda oocytes.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Animal Physiology and Development, Institute of Experimental Biology, Adam Mickiewicz University, Umultowska Str, 89, 61-614 Pozna├▒, Poland. czarniew@amu.edu.pl

ABSTRACT

Background: We report the expression pattern of 5S rDNA in the eggs of water frogs Rana lessonae, Rana ridibunda and Rana esculenta using the quantitative real-time PCR. This kind of research had never been performed before.

Results: 5S rDNA relative expression of the Rana ridibunda oocytes is approximately six times higher in comparison to the Rana lessonae oocytes. The oocytes of the investigated Rana esculenta frogs, in respect of 5S rDNA relative expression ratio, were very similar to the Rana ridibunda oocytes.

Conclusion: We suggest the possibility of using 5S rDNA as the internal control gene, in the studies of relative mRNA quantitative assays in water frog oocytes, because of its characteristic specific expression pattern in the Rana lessonae, Rana ridibunda and Rana esculenta oocytes.

No MeSH data available.


Comparison of the nucleotide sequence of 18S rDNA fragment between the R. lessonae, R. ridibunda and R. amurensis.
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Figure 2: Comparison of the nucleotide sequence of 18S rDNA fragment between the R. lessonae, R. ridibunda and R. amurensis.

Mentions: In the present study, for the first time in the research on amphibians, we examined the expression level of 5S rDNA in oocytes of water frogs. We used 18S rDNA as a reference gene. The specificity of the RT-PCR products was confirmed by sequencing reactions. Alignment of the 5S rDNA sequences of LL, RR, R. catesbeiana ([GenBank:X58367]; [10]) and R. pipiens ([GenBank:X58368]; [10]) is shown in Fig. 1, whereas comparison of a highly conserved region of the 18S rDNA of LL, RR and R. amurensis ([GenBank:AF542043.]; [11]) is shown in Fig. 2. The analysed 5S rDNA of LL ([GenBank:FJ572051]) and RR ([GenBank:FJ572052]) showed 95% and 93% sequence homology to the respective 5S rDNA of R. catesbeiana and R. pipiens. The LL ([GenBank:FJ572053]) and RR ([GenBank:FJ572054]) 18S rDNA nucleotide sequences showed 96% and 93% sequence identity to R. amurensis 18S rDNA, respectively.


Expression of 5S rDNA in the oocytes of water frogs.

Czarniewska E, Plewa R - BMC Res Notes (2009)

Comparison of the nucleotide sequence of 18S rDNA fragment between the R. lessonae, R. ridibunda and R. amurensis.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2651904&req=5

Figure 2: Comparison of the nucleotide sequence of 18S rDNA fragment between the R. lessonae, R. ridibunda and R. amurensis.
Mentions: In the present study, for the first time in the research on amphibians, we examined the expression level of 5S rDNA in oocytes of water frogs. We used 18S rDNA as a reference gene. The specificity of the RT-PCR products was confirmed by sequencing reactions. Alignment of the 5S rDNA sequences of LL, RR, R. catesbeiana ([GenBank:X58367]; [10]) and R. pipiens ([GenBank:X58368]; [10]) is shown in Fig. 1, whereas comparison of a highly conserved region of the 18S rDNA of LL, RR and R. amurensis ([GenBank:AF542043.]; [11]) is shown in Fig. 2. The analysed 5S rDNA of LL ([GenBank:FJ572051]) and RR ([GenBank:FJ572052]) showed 95% and 93% sequence homology to the respective 5S rDNA of R. catesbeiana and R. pipiens. The LL ([GenBank:FJ572053]) and RR ([GenBank:FJ572054]) 18S rDNA nucleotide sequences showed 96% and 93% sequence identity to R. amurensis 18S rDNA, respectively.

Bottom Line: We report the expression pattern of 5S rDNA in the eggs of water frogs Rana lessonae, Rana ridibunda and Rana esculenta using the quantitative real-time PCR.This kind of research had never been performed before. 5S rDNA relative expression of the Rana ridibunda oocytes is approximately six times higher in comparison to the Rana lessonae oocytes.The oocytes of the investigated Rana esculenta frogs, in respect of 5S rDNA relative expression ratio, were very similar to the Rana ridibunda oocytes.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Animal Physiology and Development, Institute of Experimental Biology, Adam Mickiewicz University, Umultowska Str, 89, 61-614 Pozna├▒, Poland. czarniew@amu.edu.pl

ABSTRACT

Background: We report the expression pattern of 5S rDNA in the eggs of water frogs Rana lessonae, Rana ridibunda and Rana esculenta using the quantitative real-time PCR. This kind of research had never been performed before.

Results: 5S rDNA relative expression of the Rana ridibunda oocytes is approximately six times higher in comparison to the Rana lessonae oocytes. The oocytes of the investigated Rana esculenta frogs, in respect of 5S rDNA relative expression ratio, were very similar to the Rana ridibunda oocytes.

Conclusion: We suggest the possibility of using 5S rDNA as the internal control gene, in the studies of relative mRNA quantitative assays in water frog oocytes, because of its characteristic specific expression pattern in the Rana lessonae, Rana ridibunda and Rana esculenta oocytes.

No MeSH data available.