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A synthetic mammalian electro-genetic transcription circuit.

Weber W, Luzi S, Karlsson M, Sanchez-Bustamante CD, Frey U, Hierlemann A, Fussenegger M - Nucleic Acids Res. (2009)

Bottom Line: Electric signal processing has evolved to manage rapid information transfer in neuronal networks and muscular contraction in multicellular organisms and controls the most sophisticated man-built devices.Using a synthetic biology approach to assemble electronic parts with genetic control units engineered into mammalian cells, we designed an electric power-adjustable transcription control circuit able to integrate the intensity of a direct current over time, to translate the amplitude or frequency of an alternating current into an adjustable genetic readout or to modulate the beating frequency of primary heart cells.Successful miniaturization of the electro-genetic devices may pave the way for the design of novel hybrid electro-genetic implants assembled from electronic and genetic parts.

View Article: PubMed Central - PubMed

Affiliation: ETH Zurich, Department of Biosystems Science and Engineering, Mattenstrasse 26, CH-4058 Basel, Switzerland.

ABSTRACT
Electric signal processing has evolved to manage rapid information transfer in neuronal networks and muscular contraction in multicellular organisms and controls the most sophisticated man-built devices. Using a synthetic biology approach to assemble electronic parts with genetic control units engineered into mammalian cells, we designed an electric power-adjustable transcription control circuit able to integrate the intensity of a direct current over time, to translate the amplitude or frequency of an alternating current into an adjustable genetic readout or to modulate the beating frequency of primary heart cells. Successful miniaturization of the electro-genetic devices may pave the way for the design of novel hybrid electro-genetic implants assembled from electronic and genetic parts.

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Electro-genetic circuits. (a) Mammalian cell-based integrator. The CPU was connected to DC of different intensities and for various periods of time; the product of time and current intensity was kept constant (t × I = constant). The resulting acetaldehyde levels and electric output signals were scored after 24 h. (b) Mammalian cell-based AM detector. The CPU was connected to an AC of 50 Hz and different intensities for 1 h and the acetaldehyde levels as well as the electric output current were quantified after 24 h. (c) Mammalian cell-based FM detector. The CPU was connected to an AC of 50 mA and different frequencies for 1 h and the acetaldehyde levels as well as the electric output current were quantified after 48 h.
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Figure 2: Electro-genetic circuits. (a) Mammalian cell-based integrator. The CPU was connected to DC of different intensities and for various periods of time; the product of time and current intensity was kept constant (t × I = constant). The resulting acetaldehyde levels and electric output signals were scored after 24 h. (b) Mammalian cell-based AM detector. The CPU was connected to an AC of 50 Hz and different intensities for 1 h and the acetaldehyde levels as well as the electric output current were quantified after 24 h. (c) Mammalian cell-based FM detector. The CPU was connected to an AC of 50 mA and different frequencies for 1 h and the acetaldehyde levels as well as the electric output current were quantified after 48 h.

Mentions: Within its linear operation range (30 mA DC ≤ input current ≤ 140 mA DC, see above), the electro-genetic device functions as a cell-based electronic integrator mimetic since electrochemical acetaldehyde production is a direct function of the exposure time and the intensity of the current. For validation of the integrator characteristics, the electro-genetic device was connected for different periods of time to currents with varying intensities, while the overall amount of electron flux was kept constant (I × t = constant). The observation that acetaldehyde levels as well as the electric output were identical for all time profiles, suggests that the device has the capacity to process electronic signals in an integrator-like manner (Figure 2a).Figure 2.


A synthetic mammalian electro-genetic transcription circuit.

Weber W, Luzi S, Karlsson M, Sanchez-Bustamante CD, Frey U, Hierlemann A, Fussenegger M - Nucleic Acids Res. (2009)

Electro-genetic circuits. (a) Mammalian cell-based integrator. The CPU was connected to DC of different intensities and for various periods of time; the product of time and current intensity was kept constant (t × I = constant). The resulting acetaldehyde levels and electric output signals were scored after 24 h. (b) Mammalian cell-based AM detector. The CPU was connected to an AC of 50 Hz and different intensities for 1 h and the acetaldehyde levels as well as the electric output current were quantified after 24 h. (c) Mammalian cell-based FM detector. The CPU was connected to an AC of 50 mA and different frequencies for 1 h and the acetaldehyde levels as well as the electric output current were quantified after 48 h.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2651811&req=5

Figure 2: Electro-genetic circuits. (a) Mammalian cell-based integrator. The CPU was connected to DC of different intensities and for various periods of time; the product of time and current intensity was kept constant (t × I = constant). The resulting acetaldehyde levels and electric output signals were scored after 24 h. (b) Mammalian cell-based AM detector. The CPU was connected to an AC of 50 Hz and different intensities for 1 h and the acetaldehyde levels as well as the electric output current were quantified after 24 h. (c) Mammalian cell-based FM detector. The CPU was connected to an AC of 50 mA and different frequencies for 1 h and the acetaldehyde levels as well as the electric output current were quantified after 48 h.
Mentions: Within its linear operation range (30 mA DC ≤ input current ≤ 140 mA DC, see above), the electro-genetic device functions as a cell-based electronic integrator mimetic since electrochemical acetaldehyde production is a direct function of the exposure time and the intensity of the current. For validation of the integrator characteristics, the electro-genetic device was connected for different periods of time to currents with varying intensities, while the overall amount of electron flux was kept constant (I × t = constant). The observation that acetaldehyde levels as well as the electric output were identical for all time profiles, suggests that the device has the capacity to process electronic signals in an integrator-like manner (Figure 2a).Figure 2.

Bottom Line: Electric signal processing has evolved to manage rapid information transfer in neuronal networks and muscular contraction in multicellular organisms and controls the most sophisticated man-built devices.Using a synthetic biology approach to assemble electronic parts with genetic control units engineered into mammalian cells, we designed an electric power-adjustable transcription control circuit able to integrate the intensity of a direct current over time, to translate the amplitude or frequency of an alternating current into an adjustable genetic readout or to modulate the beating frequency of primary heart cells.Successful miniaturization of the electro-genetic devices may pave the way for the design of novel hybrid electro-genetic implants assembled from electronic and genetic parts.

View Article: PubMed Central - PubMed

Affiliation: ETH Zurich, Department of Biosystems Science and Engineering, Mattenstrasse 26, CH-4058 Basel, Switzerland.

ABSTRACT
Electric signal processing has evolved to manage rapid information transfer in neuronal networks and muscular contraction in multicellular organisms and controls the most sophisticated man-built devices. Using a synthetic biology approach to assemble electronic parts with genetic control units engineered into mammalian cells, we designed an electric power-adjustable transcription control circuit able to integrate the intensity of a direct current over time, to translate the amplitude or frequency of an alternating current into an adjustable genetic readout or to modulate the beating frequency of primary heart cells. Successful miniaturization of the electro-genetic devices may pave the way for the design of novel hybrid electro-genetic implants assembled from electronic and genetic parts.

Show MeSH
Related in: MedlinePlus