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A new on-axis multimode spectrometer for the macromolecular crystallography beamlines of the Swiss Light Source.

Owen RL, Pearson AR, Meents A, Boehler P, Thominet V, Schulze-Briese C - J Synchrotron Radiat (2009)

Bottom Line: In situ spectroscopic methods such as UV-Vis absorption and (resonance) Raman can provide this, and can also provide a means of detecting X-ray-induced changes.Here, preliminary results are introduced from an on-axis UV-Vis absorption and Raman multimode spectrometer currently being integrated into the beamline environment at X10SA of the Swiss Light Source.The continuing development of the spectrometer is also outlined.

View Article: PubMed Central - HTML - PubMed

Affiliation: Swiss Light Source, Paul Scherrer Institute, CH-5232 Villigen PSI, Switzerland.

ABSTRACT
X-ray crystallography at third-generation synchrotron sources permits tremendous insight into the three-dimensional structure of macromolecules. Additional information is, however, often required to aid the transition from structure to function. In situ spectroscopic methods such as UV-Vis absorption and (resonance) Raman can provide this, and can also provide a means of detecting X-ray-induced changes. Here, preliminary results are introduced from an on-axis UV-Vis absorption and Raman multimode spectrometer currently being integrated into the beamline environment at X10SA of the Swiss Light Source. The continuing development of the spectrometer is also outlined.

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The branched nature of the SLS multimode spectrometer. The alignment branch (aqua) delivers light via infinity focused zoom optics to a CCD camera. The spectroscopy branch further divides into a branch for delivery of laser light for Raman spectroscopy (green) and a branch for collection of scattered light for all spectroscopies (blue).
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fig3: The branched nature of the SLS multimode spectrometer. The alignment branch (aqua) delivers light via infinity focused zoom optics to a CCD camera. The spectroscopy branch further divides into a branch for delivery of laser light for Raman spectroscopy (green) and a branch for collection of scattered light for all spectroscopies (blue).

Mentions: For the SLS-MS, a high-magnification reflective Schwarz­schild objective (Newport, 15× magnification, f = 13 mm, numerical aperture 0.4) is used. This has the advantage of freeing up a large amount of space around the sample environment as the same objective can be used for both sample alignment and spectroscopy. Reflective objectives combine several desirable characteristics for both sample alignment and spectroscopy, including zero chromatic aberration, a high laser power threshold and a long working distance. Light collected by the reflective objective is reflected in a direction 90° below the X-ray axis to the branched SLS-MS (Fig. 3 ▶).


A new on-axis multimode spectrometer for the macromolecular crystallography beamlines of the Swiss Light Source.

Owen RL, Pearson AR, Meents A, Boehler P, Thominet V, Schulze-Briese C - J Synchrotron Radiat (2009)

The branched nature of the SLS multimode spectrometer. The alignment branch (aqua) delivers light via infinity focused zoom optics to a CCD camera. The spectroscopy branch further divides into a branch for delivery of laser light for Raman spectroscopy (green) and a branch for collection of scattered light for all spectroscopies (blue).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2651763&req=5

fig3: The branched nature of the SLS multimode spectrometer. The alignment branch (aqua) delivers light via infinity focused zoom optics to a CCD camera. The spectroscopy branch further divides into a branch for delivery of laser light for Raman spectroscopy (green) and a branch for collection of scattered light for all spectroscopies (blue).
Mentions: For the SLS-MS, a high-magnification reflective Schwarz­schild objective (Newport, 15× magnification, f = 13 mm, numerical aperture 0.4) is used. This has the advantage of freeing up a large amount of space around the sample environment as the same objective can be used for both sample alignment and spectroscopy. Reflective objectives combine several desirable characteristics for both sample alignment and spectroscopy, including zero chromatic aberration, a high laser power threshold and a long working distance. Light collected by the reflective objective is reflected in a direction 90° below the X-ray axis to the branched SLS-MS (Fig. 3 ▶).

Bottom Line: In situ spectroscopic methods such as UV-Vis absorption and (resonance) Raman can provide this, and can also provide a means of detecting X-ray-induced changes.Here, preliminary results are introduced from an on-axis UV-Vis absorption and Raman multimode spectrometer currently being integrated into the beamline environment at X10SA of the Swiss Light Source.The continuing development of the spectrometer is also outlined.

View Article: PubMed Central - HTML - PubMed

Affiliation: Swiss Light Source, Paul Scherrer Institute, CH-5232 Villigen PSI, Switzerland.

ABSTRACT
X-ray crystallography at third-generation synchrotron sources permits tremendous insight into the three-dimensional structure of macromolecules. Additional information is, however, often required to aid the transition from structure to function. In situ spectroscopic methods such as UV-Vis absorption and (resonance) Raman can provide this, and can also provide a means of detecting X-ray-induced changes. Here, preliminary results are introduced from an on-axis UV-Vis absorption and Raman multimode spectrometer currently being integrated into the beamline environment at X10SA of the Swiss Light Source. The continuing development of the spectrometer is also outlined.

Show MeSH