Limits...
A new on-axis multimode spectrometer for the macromolecular crystallography beamlines of the Swiss Light Source.

Owen RL, Pearson AR, Meents A, Boehler P, Thominet V, Schulze-Briese C - J Synchrotron Radiat (2009)

Bottom Line: In situ spectroscopic methods such as UV-Vis absorption and (resonance) Raman can provide this, and can also provide a means of detecting X-ray-induced changes.Here, preliminary results are introduced from an on-axis UV-Vis absorption and Raman multimode spectrometer currently being integrated into the beamline environment at X10SA of the Swiss Light Source.The continuing development of the spectrometer is also outlined.

View Article: PubMed Central - HTML - PubMed

Affiliation: Swiss Light Source, Paul Scherrer Institute, CH-5232 Villigen PSI, Switzerland.

ABSTRACT
X-ray crystallography at third-generation synchrotron sources permits tremendous insight into the three-dimensional structure of macromolecules. Additional information is, however, often required to aid the transition from structure to function. In situ spectroscopic methods such as UV-Vis absorption and (resonance) Raman can provide this, and can also provide a means of detecting X-ray-induced changes. Here, preliminary results are introduced from an on-axis UV-Vis absorption and Raman multimode spectrometer currently being integrated into the beamline environment at X10SA of the Swiss Light Source. The continuing development of the spectrometer is also outlined.

Show MeSH

Related in: MedlinePlus

Manifestations of radiation damage over a wide range of time scales: UV–Vis absorption spectra of irradiated ethylene glycol [data collected at the ESRF using the arrangement described by Southworth-Davies & Garman (2007 ▶)] showing a rapid increase in absorption owing to  upon X-ray irradiation; disulphide bond breakage in low-dose composite data sets of DsbA (R. L. Owen, unpublished data); decay of diffracting power of holoferritin crystals as a function of X-ray dose (Owen et al., 2006 ▶).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2651763&req=5

fig1: Manifestations of radiation damage over a wide range of time scales: UV–Vis absorption spectra of irradiated ethylene glycol [data collected at the ESRF using the arrangement described by Southworth-Davies & Garman (2007 ▶)] showing a rapid increase in absorption owing to upon X-ray irradiation; disulphide bond breakage in low-dose composite data sets of DsbA (R. L. Owen, unpublished data); decay of diffracting power of holoferritin crystals as a function of X-ray dose (Owen et al., 2006 ▶).

Mentions: These changes are illustrated in Fig. 1 ▶ and range from a near instantaneous generation of aqueous, or solvated, electrons () upon X-ray exposure, through fast changes at electron-sensitive centres such as redox sites or disulphide bonds at low X-ray dose, to the eventual decay of diffraction and crystal death. These radiation-induced changes have been observed in a variety of biological systems (for example, see Berglund et al., 2002 ▶; Adam et al., 2004 ▶; Hough et al., 2008 ▶; Pearson et al., 2007 ▶).


A new on-axis multimode spectrometer for the macromolecular crystallography beamlines of the Swiss Light Source.

Owen RL, Pearson AR, Meents A, Boehler P, Thominet V, Schulze-Briese C - J Synchrotron Radiat (2009)

Manifestations of radiation damage over a wide range of time scales: UV–Vis absorption spectra of irradiated ethylene glycol [data collected at the ESRF using the arrangement described by Southworth-Davies & Garman (2007 ▶)] showing a rapid increase in absorption owing to  upon X-ray irradiation; disulphide bond breakage in low-dose composite data sets of DsbA (R. L. Owen, unpublished data); decay of diffracting power of holoferritin crystals as a function of X-ray dose (Owen et al., 2006 ▶).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2651763&req=5

fig1: Manifestations of radiation damage over a wide range of time scales: UV–Vis absorption spectra of irradiated ethylene glycol [data collected at the ESRF using the arrangement described by Southworth-Davies & Garman (2007 ▶)] showing a rapid increase in absorption owing to upon X-ray irradiation; disulphide bond breakage in low-dose composite data sets of DsbA (R. L. Owen, unpublished data); decay of diffracting power of holoferritin crystals as a function of X-ray dose (Owen et al., 2006 ▶).
Mentions: These changes are illustrated in Fig. 1 ▶ and range from a near instantaneous generation of aqueous, or solvated, electrons () upon X-ray exposure, through fast changes at electron-sensitive centres such as redox sites or disulphide bonds at low X-ray dose, to the eventual decay of diffraction and crystal death. These radiation-induced changes have been observed in a variety of biological systems (for example, see Berglund et al., 2002 ▶; Adam et al., 2004 ▶; Hough et al., 2008 ▶; Pearson et al., 2007 ▶).

Bottom Line: In situ spectroscopic methods such as UV-Vis absorption and (resonance) Raman can provide this, and can also provide a means of detecting X-ray-induced changes.Here, preliminary results are introduced from an on-axis UV-Vis absorption and Raman multimode spectrometer currently being integrated into the beamline environment at X10SA of the Swiss Light Source.The continuing development of the spectrometer is also outlined.

View Article: PubMed Central - HTML - PubMed

Affiliation: Swiss Light Source, Paul Scherrer Institute, CH-5232 Villigen PSI, Switzerland.

ABSTRACT
X-ray crystallography at third-generation synchrotron sources permits tremendous insight into the three-dimensional structure of macromolecules. Additional information is, however, often required to aid the transition from structure to function. In situ spectroscopic methods such as UV-Vis absorption and (resonance) Raman can provide this, and can also provide a means of detecting X-ray-induced changes. Here, preliminary results are introduced from an on-axis UV-Vis absorption and Raman multimode spectrometer currently being integrated into the beamline environment at X10SA of the Swiss Light Source. The continuing development of the spectrometer is also outlined.

Show MeSH
Related in: MedlinePlus