Limits...
Fabrication of massive sheets of single layer patterned arrays using lipid directed reengineered phi29 motor dodecamer.

Xiao F, Sun J, Coban O, Schoen P, Wang JC, Cheng RH, Guo P - ACS Nano (2009)

Bottom Line: The ability to produce a single layer array of biological structures with high replication fidelity represents a significant advance in the area of nanomimetics.A thin lipid monolayer was used to direct the assembly of massive sheets of single layer patterned arrays of the reengineered motor dodecamer.Uniform, clean and highly ordered arrays were constructed as shown by both transmission electron microscopy and atomic force microscopy imaging.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, University of Cincinnati, Cincinnati, Ohio 45221, USA.

ABSTRACT
The bottom-up assembly of patterned arrays is an exciting and important area in current nanotechnology. Arrays can be engineered to serve as components in chips for a virtually inexhaustible list of applications ranging from disease diagnosis to ultra-high-density data storage. Phi29 motor dodecamer has been reported to form elegant multilayer tetragonal arrays. However, multilayer protein arrays are of limited use for nanotechnological applications which demand nanoreplica or coating technologies. The ability to produce a single layer array of biological structures with high replication fidelity represents a significant advance in the area of nanomimetics. In this paper, we report on the assembly of single layer sheets of reengineered phi29 motor dodecamer. A thin lipid monolayer was used to direct the assembly of massive sheets of single layer patterned arrays of the reengineered motor dodecamer. Uniform, clean and highly ordered arrays were constructed as shown by both transmission electron microscopy and atomic force microscopy imaging.

Show MeSH

Related in: MedlinePlus

Multilayer versus single layer sheet arrays of phi29 motor dodecamer. (A) Side view of a multiple layer dodecamer array showing the horizontal face-up and face-down arrangements and the vertical head-to-tail alignment which leads to multiple layers overlap. (B) Side view of a single layer dodecamer array displaying an alternating face-up and face-down arrangement. (C) Native phi29 motor dodecamer (inset) assembled into ordered multiple layer structures as shown by negative-stain electron micrograph. (D) The negative-stain electron micrograph of reengineered phi29 motor dodecamer (inset) arrays shows that a single layer sheet was formed. (E) Projection density map of the single layer of motor dodecamers and the Fourier transform (inset). The unit cell is rectangular, with a lattice constant of ∼20 nm. The alternate orientations of the dodecamer can be observed. (F) AFM image of N-strep dodecamer arrays and a line scan across crystalline area with lattice defects (inset). The height difference between the top dodecamer layer and mica surface is ∼7.5 nm, which corresponds to a single dodecamer layer.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2651733&req=5

fig1: Multilayer versus single layer sheet arrays of phi29 motor dodecamer. (A) Side view of a multiple layer dodecamer array showing the horizontal face-up and face-down arrangements and the vertical head-to-tail alignment which leads to multiple layers overlap. (B) Side view of a single layer dodecamer array displaying an alternating face-up and face-down arrangement. (C) Native phi29 motor dodecamer (inset) assembled into ordered multiple layer structures as shown by negative-stain electron micrograph. (D) The negative-stain electron micrograph of reengineered phi29 motor dodecamer (inset) arrays shows that a single layer sheet was formed. (E) Projection density map of the single layer of motor dodecamers and the Fourier transform (inset). The unit cell is rectangular, with a lattice constant of ∼20 nm. The alternate orientations of the dodecamer can be observed. (F) AFM image of N-strep dodecamer arrays and a line scan across crystalline area with lattice defects (inset). The height difference between the top dodecamer layer and mica surface is ∼7.5 nm, which corresponds to a single dodecamer layer.

Mentions: The formation of multilayer arrays is driven by two distinct protein interaction mechanisms. First, horizontal side-by-side interactions between individual dodecamers allow for the extension and growth of a two-dimensional layer. Second, interactions between the narrow and the wide ends of dodecamer molecules promote the buildup of multiple layers vertically (1). To facilitate the formation of a single layer and prevent the continuous growth of multiple layers, a short peptide sequence was introduced either into the gp10 N- or C-terminus, located at the narrow and wide end of the dodecamer, respectively (1).


Fabrication of massive sheets of single layer patterned arrays using lipid directed reengineered phi29 motor dodecamer.

Xiao F, Sun J, Coban O, Schoen P, Wang JC, Cheng RH, Guo P - ACS Nano (2009)

Multilayer versus single layer sheet arrays of phi29 motor dodecamer. (A) Side view of a multiple layer dodecamer array showing the horizontal face-up and face-down arrangements and the vertical head-to-tail alignment which leads to multiple layers overlap. (B) Side view of a single layer dodecamer array displaying an alternating face-up and face-down arrangement. (C) Native phi29 motor dodecamer (inset) assembled into ordered multiple layer structures as shown by negative-stain electron micrograph. (D) The negative-stain electron micrograph of reengineered phi29 motor dodecamer (inset) arrays shows that a single layer sheet was formed. (E) Projection density map of the single layer of motor dodecamers and the Fourier transform (inset). The unit cell is rectangular, with a lattice constant of ∼20 nm. The alternate orientations of the dodecamer can be observed. (F) AFM image of N-strep dodecamer arrays and a line scan across crystalline area with lattice defects (inset). The height difference between the top dodecamer layer and mica surface is ∼7.5 nm, which corresponds to a single dodecamer layer.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2651733&req=5

fig1: Multilayer versus single layer sheet arrays of phi29 motor dodecamer. (A) Side view of a multiple layer dodecamer array showing the horizontal face-up and face-down arrangements and the vertical head-to-tail alignment which leads to multiple layers overlap. (B) Side view of a single layer dodecamer array displaying an alternating face-up and face-down arrangement. (C) Native phi29 motor dodecamer (inset) assembled into ordered multiple layer structures as shown by negative-stain electron micrograph. (D) The negative-stain electron micrograph of reengineered phi29 motor dodecamer (inset) arrays shows that a single layer sheet was formed. (E) Projection density map of the single layer of motor dodecamers and the Fourier transform (inset). The unit cell is rectangular, with a lattice constant of ∼20 nm. The alternate orientations of the dodecamer can be observed. (F) AFM image of N-strep dodecamer arrays and a line scan across crystalline area with lattice defects (inset). The height difference between the top dodecamer layer and mica surface is ∼7.5 nm, which corresponds to a single dodecamer layer.
Mentions: The formation of multilayer arrays is driven by two distinct protein interaction mechanisms. First, horizontal side-by-side interactions between individual dodecamers allow for the extension and growth of a two-dimensional layer. Second, interactions between the narrow and the wide ends of dodecamer molecules promote the buildup of multiple layers vertically (1). To facilitate the formation of a single layer and prevent the continuous growth of multiple layers, a short peptide sequence was introduced either into the gp10 N- or C-terminus, located at the narrow and wide end of the dodecamer, respectively (1).

Bottom Line: The ability to produce a single layer array of biological structures with high replication fidelity represents a significant advance in the area of nanomimetics.A thin lipid monolayer was used to direct the assembly of massive sheets of single layer patterned arrays of the reengineered motor dodecamer.Uniform, clean and highly ordered arrays were constructed as shown by both transmission electron microscopy and atomic force microscopy imaging.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, University of Cincinnati, Cincinnati, Ohio 45221, USA.

ABSTRACT
The bottom-up assembly of patterned arrays is an exciting and important area in current nanotechnology. Arrays can be engineered to serve as components in chips for a virtually inexhaustible list of applications ranging from disease diagnosis to ultra-high-density data storage. Phi29 motor dodecamer has been reported to form elegant multilayer tetragonal arrays. However, multilayer protein arrays are of limited use for nanotechnological applications which demand nanoreplica or coating technologies. The ability to produce a single layer array of biological structures with high replication fidelity represents a significant advance in the area of nanomimetics. In this paper, we report on the assembly of single layer sheets of reengineered phi29 motor dodecamer. A thin lipid monolayer was used to direct the assembly of massive sheets of single layer patterned arrays of the reengineered motor dodecamer. Uniform, clean and highly ordered arrays were constructed as shown by both transmission electron microscopy and atomic force microscopy imaging.

Show MeSH
Related in: MedlinePlus