Limits...
Utilization of murine laparoscopy for continuous in-vivo assessment of the liver in multiple disease models.

Shapira Y, Katz M, Ali M, Kaplan M, Brazowski E, Halpern Z, Elinav E - PLoS ONE (2009)

Bottom Line: Current strategies for follow up of murine models of liver disease are flawed by inability to continuously monitor disease progression in the tissue level, and necessitate sacrifice of animals for tissue sampling.In this study we aimed at developing a safe repetitive tool for sampling livers in vivo, by utilization of a miniaturized endoscopy system for laparoscopic liver biopsies and for injection of tumor cells into livers.The system enables safe and repeated liver biopsies in mice and rats, yielding adequate tissue for histological staining and RNA extraction.

View Article: PubMed Central - PubMed

Affiliation: Institute for Gastroenterology and Liver Disease, Tel Aviv Sourasky Medical Center, Tel Aviv, Israel.

ABSTRACT

Background: Current strategies for follow up of murine models of liver disease are flawed by inability to continuously monitor disease progression in the tissue level, and necessitate sacrifice of animals for tissue sampling.

Aims: In this study we aimed at developing a safe repetitive tool for sampling livers in vivo, by utilization of a miniaturized endoscopy system for laparoscopic liver biopsies and for injection of tumor cells into livers.

Results: We report the development of a protocol for murine laparoscopy that allows repeated visualization of murine intra-abdominal organs. The system enables safe and repeated liver biopsies in mice and rats, yielding adequate tissue for histological staining and RNA extraction. In addition, injection of tumor cells into livers facilitates under-vision implantation of hepatic tumors in liver, followed by visualization of tumor growth.

Conclusions: Murine laparoscopy may be employed as a novel imaging modality for continuous assessment and manipulation of chronic liver disease models.

Show MeSH

Related in: MedlinePlus

Laparoscopic imaging of healthy and diseased livers.A – Healthy liver, B – MCD Diet-induced NASH (with biopsy forceps in frame), C – TAA-induced liver fibrosis.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2651645&req=5

pone-0004776-g004: Laparoscopic imaging of healthy and diseased livers.A – Healthy liver, B – MCD Diet-induced NASH (with biopsy forceps in frame), C – TAA-induced liver fibrosis.

Mentions: As is depicted in figure 3 and video S2, high resolution imaging of the liver in health and in various disease states could be achieved using murine laparoscopy. While healthy livers appear violet in color and smooth in texture (figure 4A), severe steatosis results in white discoloration (figure 4B), while fibrosis results in a gray discoloration of the liver and intense granulation and irregularities of its surface (figure 4C). In tumor models, subscapular masses could be easily detected within days of implantation (see below).


Utilization of murine laparoscopy for continuous in-vivo assessment of the liver in multiple disease models.

Shapira Y, Katz M, Ali M, Kaplan M, Brazowski E, Halpern Z, Elinav E - PLoS ONE (2009)

Laparoscopic imaging of healthy and diseased livers.A – Healthy liver, B – MCD Diet-induced NASH (with biopsy forceps in frame), C – TAA-induced liver fibrosis.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2651645&req=5

pone-0004776-g004: Laparoscopic imaging of healthy and diseased livers.A – Healthy liver, B – MCD Diet-induced NASH (with biopsy forceps in frame), C – TAA-induced liver fibrosis.
Mentions: As is depicted in figure 3 and video S2, high resolution imaging of the liver in health and in various disease states could be achieved using murine laparoscopy. While healthy livers appear violet in color and smooth in texture (figure 4A), severe steatosis results in white discoloration (figure 4B), while fibrosis results in a gray discoloration of the liver and intense granulation and irregularities of its surface (figure 4C). In tumor models, subscapular masses could be easily detected within days of implantation (see below).

Bottom Line: Current strategies for follow up of murine models of liver disease are flawed by inability to continuously monitor disease progression in the tissue level, and necessitate sacrifice of animals for tissue sampling.In this study we aimed at developing a safe repetitive tool for sampling livers in vivo, by utilization of a miniaturized endoscopy system for laparoscopic liver biopsies and for injection of tumor cells into livers.The system enables safe and repeated liver biopsies in mice and rats, yielding adequate tissue for histological staining and RNA extraction.

View Article: PubMed Central - PubMed

Affiliation: Institute for Gastroenterology and Liver Disease, Tel Aviv Sourasky Medical Center, Tel Aviv, Israel.

ABSTRACT

Background: Current strategies for follow up of murine models of liver disease are flawed by inability to continuously monitor disease progression in the tissue level, and necessitate sacrifice of animals for tissue sampling.

Aims: In this study we aimed at developing a safe repetitive tool for sampling livers in vivo, by utilization of a miniaturized endoscopy system for laparoscopic liver biopsies and for injection of tumor cells into livers.

Results: We report the development of a protocol for murine laparoscopy that allows repeated visualization of murine intra-abdominal organs. The system enables safe and repeated liver biopsies in mice and rats, yielding adequate tissue for histological staining and RNA extraction. In addition, injection of tumor cells into livers facilitates under-vision implantation of hepatic tumors in liver, followed by visualization of tumor growth.

Conclusions: Murine laparoscopy may be employed as a novel imaging modality for continuous assessment and manipulation of chronic liver disease models.

Show MeSH
Related in: MedlinePlus