Limits...
Aptamer-targeted gold nanoparticles as molecular-specific contrast agents for reflectance imaging.

Javier DJ, Nitin N, Levy M, Ellington A, Richards-Kortum R - Bioconjug. Chem. (2008)

Bottom Line: We devised a novel conjugation approach using an extended aptamer design where the extension is complementary to an oligonucleotide sequence attached to the surface of the gold nanoparticles.We demonstrate this conjugation approach to create a contrast agent designed for detection of prostate-specific membrane antigen (PSMA), obtaining reflectance images of PSMA(+) and PSMA(-) cell lines treated with the anti-PSMA aptamer-gold conjugates.This design strategy can easily be modified to incorporate multifunctional agents as part of a multimodal platform for reflectance imaging applications.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioengineering, Rice University, Houston, Texas 77005, USA.

ABSTRACT
Targeted metallic nanoparticles have shown potential as a platform for development of molecular-specific contrast agents. Aptamers have recently been demonstrated as ideal candidates for molecular targeting applications. In this study, we investigated the development of aptamer-based gold nanoparticles as contrast agents, using aptamers as targeting agents and gold nanoparticles as imaging agents. We devised a novel conjugation approach using an extended aptamer design where the extension is complementary to an oligonucleotide sequence attached to the surface of the gold nanoparticles. The chemical and optical properties of the aptamer-gold conjugates were characterized using size measurements and oligonucleotide quantitation assays. We demonstrate this conjugation approach to create a contrast agent designed for detection of prostate-specific membrane antigen (PSMA), obtaining reflectance images of PSMA(+) and PSMA(-) cell lines treated with the anti-PSMA aptamer-gold conjugates. This design strategy can easily be modified to incorporate multifunctional agents as part of a multimodal platform for reflectance imaging applications.

Show MeSH
Schematic diagram for the conjugation of anti-PSMA aptamers to gold nanoparticles.
© Copyright Policy - open-access - ccc-price
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2651625&req=5

fig1: Schematic diagram for the conjugation of anti-PSMA aptamers to gold nanoparticles.

Mentions: In a previous study, we demonstrated the development of aptamer-based quantum dot conjugates for molecular imaging where the conjugation of the aptamers to the quantum dot is facilitated using biotin−avidin interactions (8). In this paper, we developed aptamer-targeted gold nanoparticles for use as contrast agents for reflectance imaging. In this approach, shown schematically in Figure 1, conjugation of the aptamers to gold nanoparticles is accomplished using an extended aptamer design where the extension is complementary to an oligonucleotide sequence attached to the surface of gold nanoparticles. This novel approach provides a simple conjugation method and offers the potential for multiplexing capabilities.


Aptamer-targeted gold nanoparticles as molecular-specific contrast agents for reflectance imaging.

Javier DJ, Nitin N, Levy M, Ellington A, Richards-Kortum R - Bioconjug. Chem. (2008)

Schematic diagram for the conjugation of anti-PSMA aptamers to gold nanoparticles.
© Copyright Policy - open-access - ccc-price
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2651625&req=5

fig1: Schematic diagram for the conjugation of anti-PSMA aptamers to gold nanoparticles.
Mentions: In a previous study, we demonstrated the development of aptamer-based quantum dot conjugates for molecular imaging where the conjugation of the aptamers to the quantum dot is facilitated using biotin−avidin interactions (8). In this paper, we developed aptamer-targeted gold nanoparticles for use as contrast agents for reflectance imaging. In this approach, shown schematically in Figure 1, conjugation of the aptamers to gold nanoparticles is accomplished using an extended aptamer design where the extension is complementary to an oligonucleotide sequence attached to the surface of gold nanoparticles. This novel approach provides a simple conjugation method and offers the potential for multiplexing capabilities.

Bottom Line: We devised a novel conjugation approach using an extended aptamer design where the extension is complementary to an oligonucleotide sequence attached to the surface of the gold nanoparticles.We demonstrate this conjugation approach to create a contrast agent designed for detection of prostate-specific membrane antigen (PSMA), obtaining reflectance images of PSMA(+) and PSMA(-) cell lines treated with the anti-PSMA aptamer-gold conjugates.This design strategy can easily be modified to incorporate multifunctional agents as part of a multimodal platform for reflectance imaging applications.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioengineering, Rice University, Houston, Texas 77005, USA.

ABSTRACT
Targeted metallic nanoparticles have shown potential as a platform for development of molecular-specific contrast agents. Aptamers have recently been demonstrated as ideal candidates for molecular targeting applications. In this study, we investigated the development of aptamer-based gold nanoparticles as contrast agents, using aptamers as targeting agents and gold nanoparticles as imaging agents. We devised a novel conjugation approach using an extended aptamer design where the extension is complementary to an oligonucleotide sequence attached to the surface of the gold nanoparticles. The chemical and optical properties of the aptamer-gold conjugates were characterized using size measurements and oligonucleotide quantitation assays. We demonstrate this conjugation approach to create a contrast agent designed for detection of prostate-specific membrane antigen (PSMA), obtaining reflectance images of PSMA(+) and PSMA(-) cell lines treated with the anti-PSMA aptamer-gold conjugates. This design strategy can easily be modified to incorporate multifunctional agents as part of a multimodal platform for reflectance imaging applications.

Show MeSH