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A PLENA-like gene of peach is involved in carpel formation and subsequent transformation into a fleshy fruit.

Tadiello A, Pavanello A, Zanin D, Caporali E, Colombo L, Rotino GL, Trainotti L, Casadoro G - J. Exp. Bot. (2009)

Bottom Line: Here a detailed analysis of a gene that belongs to the PLENA subfamily of MADS-box genes is shown.Interestingly, the transgenic berries constitutively expressing the PpPLENA gene show an accelerated ripening, as judged by the expression of genes that are important for tomato fruit ripening.It is suggested that PpPLENA might interfere with the endogenous activity of TAGL1, thereby activating the fruit ripening pathway earlier compared with wild-type tomato plants.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Biologia, Università di Padova, 35131 Padova, Italy.

ABSTRACT
MADS-box genes have been shown to play a role in the formation of fruits, both in Arabidopsis and in tomato. In peach, two C-class MADS-box genes have been isolated. Both of them are expressed during flower and mesocarp development. Here a detailed analysis of a gene that belongs to the PLENA subfamily of MADS-box genes is shown. The expression of this PLENA-like gene (PpPLENA) increases during fruit ripening, and its ectopic expression in tomato plants causes the transformation of sepals into carpel-like structures that become fleshy and ripen like real fruits. Interestingly, the transgenic berries constitutively expressing the PpPLENA gene show an accelerated ripening, as judged by the expression of genes that are important for tomato fruit ripening. It is suggested that PpPLENA might interfere with the endogenous activity of TAGL1, thereby activating the fruit ripening pathway earlier compared with wild-type tomato plants.

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Related in: MedlinePlus

Relative expression profiles of ripening-related genes in ripe fleshy sepals of tomato plants overexpressing the peach PpPLE cDNA (794). Grey bars represent the values obtained for transgenic tomato sepals, while white bars indicate those of the wild type. The analysed genes are: PpPLE (794) (peach PpPLE cDNA), ACO1 (climacteric ACC oxidase), PSY1 (tomato chromoplast phytoene synthase), and PG (tomato ripening endopolygalacturonase). Values (means of the normalized expression) have been obtained by means of real-time qRT-PCR. Bars are the standard deviations from the means.
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fig5: Relative expression profiles of ripening-related genes in ripe fleshy sepals of tomato plants overexpressing the peach PpPLE cDNA (794). Grey bars represent the values obtained for transgenic tomato sepals, while white bars indicate those of the wild type. The analysed genes are: PpPLE (794) (peach PpPLE cDNA), ACO1 (climacteric ACC oxidase), PSY1 (tomato chromoplast phytoene synthase), and PG (tomato ripening endopolygalacturonase). Values (means of the normalized expression) have been obtained by means of real-time qRT-PCR. Bars are the standard deviations from the means.

Mentions: A molecular analysis comparing wild-type and transgenic fleshy sepals confirmed the morphological observations. The selected genes are usually regarded as markers of tomato fruit ripening: ACO1 codes for the ACC oxidase involved in the synthesis of climacteric ethylene (Hamilton et al., 1990; Köck et al., 1991), PSY1 codes for the phytoene synthase exclusively expressed in tomato chromoplasts (Fraser et al., 1994; Bramley 2002), while PG encodes the endopolygalacturonase highly expressed during softening (DellaPenna et al., 1986; Bird et al., 1988). In the red fleshy sepals where the peach PpPLE gene is expressed (Fig. 5), all three marker genes showed a higher transcript amount compared with mature control sepals (Fig. 5).


A PLENA-like gene of peach is involved in carpel formation and subsequent transformation into a fleshy fruit.

Tadiello A, Pavanello A, Zanin D, Caporali E, Colombo L, Rotino GL, Trainotti L, Casadoro G - J. Exp. Bot. (2009)

Relative expression profiles of ripening-related genes in ripe fleshy sepals of tomato plants overexpressing the peach PpPLE cDNA (794). Grey bars represent the values obtained for transgenic tomato sepals, while white bars indicate those of the wild type. The analysed genes are: PpPLE (794) (peach PpPLE cDNA), ACO1 (climacteric ACC oxidase), PSY1 (tomato chromoplast phytoene synthase), and PG (tomato ripening endopolygalacturonase). Values (means of the normalized expression) have been obtained by means of real-time qRT-PCR. Bars are the standard deviations from the means.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2651465&req=5

fig5: Relative expression profiles of ripening-related genes in ripe fleshy sepals of tomato plants overexpressing the peach PpPLE cDNA (794). Grey bars represent the values obtained for transgenic tomato sepals, while white bars indicate those of the wild type. The analysed genes are: PpPLE (794) (peach PpPLE cDNA), ACO1 (climacteric ACC oxidase), PSY1 (tomato chromoplast phytoene synthase), and PG (tomato ripening endopolygalacturonase). Values (means of the normalized expression) have been obtained by means of real-time qRT-PCR. Bars are the standard deviations from the means.
Mentions: A molecular analysis comparing wild-type and transgenic fleshy sepals confirmed the morphological observations. The selected genes are usually regarded as markers of tomato fruit ripening: ACO1 codes for the ACC oxidase involved in the synthesis of climacteric ethylene (Hamilton et al., 1990; Köck et al., 1991), PSY1 codes for the phytoene synthase exclusively expressed in tomato chromoplasts (Fraser et al., 1994; Bramley 2002), while PG encodes the endopolygalacturonase highly expressed during softening (DellaPenna et al., 1986; Bird et al., 1988). In the red fleshy sepals where the peach PpPLE gene is expressed (Fig. 5), all three marker genes showed a higher transcript amount compared with mature control sepals (Fig. 5).

Bottom Line: Here a detailed analysis of a gene that belongs to the PLENA subfamily of MADS-box genes is shown.Interestingly, the transgenic berries constitutively expressing the PpPLENA gene show an accelerated ripening, as judged by the expression of genes that are important for tomato fruit ripening.It is suggested that PpPLENA might interfere with the endogenous activity of TAGL1, thereby activating the fruit ripening pathway earlier compared with wild-type tomato plants.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Biologia, Università di Padova, 35131 Padova, Italy.

ABSTRACT
MADS-box genes have been shown to play a role in the formation of fruits, both in Arabidopsis and in tomato. In peach, two C-class MADS-box genes have been isolated. Both of them are expressed during flower and mesocarp development. Here a detailed analysis of a gene that belongs to the PLENA subfamily of MADS-box genes is shown. The expression of this PLENA-like gene (PpPLENA) increases during fruit ripening, and its ectopic expression in tomato plants causes the transformation of sepals into carpel-like structures that become fleshy and ripen like real fruits. Interestingly, the transgenic berries constitutively expressing the PpPLENA gene show an accelerated ripening, as judged by the expression of genes that are important for tomato fruit ripening. It is suggested that PpPLENA might interfere with the endogenous activity of TAGL1, thereby activating the fruit ripening pathway earlier compared with wild-type tomato plants.

Show MeSH
Related in: MedlinePlus