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Silencing of StKCS6 in potato periderm leads to reduced chain lengths of suberin and wax compounds and increased peridermal transpiration.

Serra O, Soler M, Hohn C, Franke R, Schreiber L, Prat S, Molinas M, Figueras M - J. Exp. Bot. (2008)

Bottom Line: All compounds with chain lengths of C(28) and higher were significantly reduced in silenced periderms, whereas compounds with chain lengths of C(26) and lower accumulated.As a result, peridermal transpiration of the silenced lines was about 1.5-times higher than that of the wild type.Our results convincingly show that StKCS6 is involved in both suberin and wax biosynthesis and that a reduction of the monomeric carbon chain lengths leads to increased rates of peridermal transpiration.

View Article: PubMed Central - PubMed

Affiliation: Laboratori del Suro, Departament de Biologia, Facultat de Ciències, Universitat de Girona, Campus Montilivi s/n, E-17071 Girona, Spain.

ABSTRACT
Very long chain aliphatic compounds occur in the suberin polymer and associated wax. Up to now only few genes involved in suberin biosynthesis have been identified. This is a report on the isolation of a potato (Solanum tuberosum) 3-ketoacyl-CoA synthase (KCS) gene and the study of its molecular and physiological relevance by means of a reverse genetic approach. This gene, called StKCS6, was stably silenced by RNA interference (RNAi) in potato. Analysis of the chemical composition of silenced potato tuber periderms indicated that StKCS6 down-regulation has a significant and fairly specific effect on the chain length distribution of very long-chain fatty acids (VLCFAs) and derivatives, occurring in the suberin polymer and peridermal wax. All compounds with chain lengths of C(28) and higher were significantly reduced in silenced periderms, whereas compounds with chain lengths of C(26) and lower accumulated. Thus, StKCS6 is preferentially involved in the formation of suberin and wax lipidic monomers with chain lengths of C(28) and higher. As a result, peridermal transpiration of the silenced lines was about 1.5-times higher than that of the wild type. Our results convincingly show that StKCS6 is involved in both suberin and wax biosynthesis and that a reduction of the monomeric carbon chain lengths leads to increased rates of peridermal transpiration.

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Related in: MedlinePlus

Ultrastructure of StKCS6-silenced periderms. Transmission electron micrographs showing a detailed view of the cork cell walls ultrastructure of wild-type (A) and transgenic StKCS6-RNAi (B) periderms. The polysaccharide primary wall (PW) and tertiary wall (TW) as well as the suberized secondary wall (SW) formed by the typical suberin lamella show a normal development in the StKCS6-RNAi periderm.
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fig6: Ultrastructure of StKCS6-silenced periderms. Transmission electron micrographs showing a detailed view of the cork cell walls ultrastructure of wild-type (A) and transgenic StKCS6-RNAi (B) periderms. The polysaccharide primary wall (PW) and tertiary wall (TW) as well as the suberized secondary wall (SW) formed by the typical suberin lamella show a normal development in the StKCS6-RNAi periderm.

Mentions: Periderm samples obtained from 21-d-stored tubers were examined using light and electron microscopy in order to test whether StKCS6 silencing had an effect on the periderm fine structure or ultrastructure. No differences could be observed using light microscopy, SEM or TEM (Fig. 6), being the ultrastructural lamellation of the suberin polymer similar in StKCS6-silenced lines and the wild type.


Silencing of StKCS6 in potato periderm leads to reduced chain lengths of suberin and wax compounds and increased peridermal transpiration.

Serra O, Soler M, Hohn C, Franke R, Schreiber L, Prat S, Molinas M, Figueras M - J. Exp. Bot. (2008)

Ultrastructure of StKCS6-silenced periderms. Transmission electron micrographs showing a detailed view of the cork cell walls ultrastructure of wild-type (A) and transgenic StKCS6-RNAi (B) periderms. The polysaccharide primary wall (PW) and tertiary wall (TW) as well as the suberized secondary wall (SW) formed by the typical suberin lamella show a normal development in the StKCS6-RNAi periderm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2651458&req=5

fig6: Ultrastructure of StKCS6-silenced periderms. Transmission electron micrographs showing a detailed view of the cork cell walls ultrastructure of wild-type (A) and transgenic StKCS6-RNAi (B) periderms. The polysaccharide primary wall (PW) and tertiary wall (TW) as well as the suberized secondary wall (SW) formed by the typical suberin lamella show a normal development in the StKCS6-RNAi periderm.
Mentions: Periderm samples obtained from 21-d-stored tubers were examined using light and electron microscopy in order to test whether StKCS6 silencing had an effect on the periderm fine structure or ultrastructure. No differences could be observed using light microscopy, SEM or TEM (Fig. 6), being the ultrastructural lamellation of the suberin polymer similar in StKCS6-silenced lines and the wild type.

Bottom Line: All compounds with chain lengths of C(28) and higher were significantly reduced in silenced periderms, whereas compounds with chain lengths of C(26) and lower accumulated.As a result, peridermal transpiration of the silenced lines was about 1.5-times higher than that of the wild type.Our results convincingly show that StKCS6 is involved in both suberin and wax biosynthesis and that a reduction of the monomeric carbon chain lengths leads to increased rates of peridermal transpiration.

View Article: PubMed Central - PubMed

Affiliation: Laboratori del Suro, Departament de Biologia, Facultat de Ciències, Universitat de Girona, Campus Montilivi s/n, E-17071 Girona, Spain.

ABSTRACT
Very long chain aliphatic compounds occur in the suberin polymer and associated wax. Up to now only few genes involved in suberin biosynthesis have been identified. This is a report on the isolation of a potato (Solanum tuberosum) 3-ketoacyl-CoA synthase (KCS) gene and the study of its molecular and physiological relevance by means of a reverse genetic approach. This gene, called StKCS6, was stably silenced by RNA interference (RNAi) in potato. Analysis of the chemical composition of silenced potato tuber periderms indicated that StKCS6 down-regulation has a significant and fairly specific effect on the chain length distribution of very long-chain fatty acids (VLCFAs) and derivatives, occurring in the suberin polymer and peridermal wax. All compounds with chain lengths of C(28) and higher were significantly reduced in silenced periderms, whereas compounds with chain lengths of C(26) and lower accumulated. Thus, StKCS6 is preferentially involved in the formation of suberin and wax lipidic monomers with chain lengths of C(28) and higher. As a result, peridermal transpiration of the silenced lines was about 1.5-times higher than that of the wild type. Our results convincingly show that StKCS6 is involved in both suberin and wax biosynthesis and that a reduction of the monomeric carbon chain lengths leads to increased rates of peridermal transpiration.

Show MeSH
Related in: MedlinePlus