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Silencing of StKCS6 in potato periderm leads to reduced chain lengths of suberin and wax compounds and increased peridermal transpiration.

Serra O, Soler M, Hohn C, Franke R, Schreiber L, Prat S, Molinas M, Figueras M - J. Exp. Bot. (2008)

Bottom Line: All compounds with chain lengths of C(28) and higher were significantly reduced in silenced periderms, whereas compounds with chain lengths of C(26) and lower accumulated.As a result, peridermal transpiration of the silenced lines was about 1.5-times higher than that of the wild type.Our results convincingly show that StKCS6 is involved in both suberin and wax biosynthesis and that a reduction of the monomeric carbon chain lengths leads to increased rates of peridermal transpiration.

View Article: PubMed Central - PubMed

Affiliation: Laboratori del Suro, Departament de Biologia, Facultat de Ciències, Universitat de Girona, Campus Montilivi s/n, E-17071 Girona, Spain.

ABSTRACT
Very long chain aliphatic compounds occur in the suberin polymer and associated wax. Up to now only few genes involved in suberin biosynthesis have been identified. This is a report on the isolation of a potato (Solanum tuberosum) 3-ketoacyl-CoA synthase (KCS) gene and the study of its molecular and physiological relevance by means of a reverse genetic approach. This gene, called StKCS6, was stably silenced by RNA interference (RNAi) in potato. Analysis of the chemical composition of silenced potato tuber periderms indicated that StKCS6 down-regulation has a significant and fairly specific effect on the chain length distribution of very long-chain fatty acids (VLCFAs) and derivatives, occurring in the suberin polymer and peridermal wax. All compounds with chain lengths of C(28) and higher were significantly reduced in silenced periderms, whereas compounds with chain lengths of C(26) and lower accumulated. Thus, StKCS6 is preferentially involved in the formation of suberin and wax lipidic monomers with chain lengths of C(28) and higher. As a result, peridermal transpiration of the silenced lines was about 1.5-times higher than that of the wild type. Our results convincingly show that StKCS6 is involved in both suberin and wax biosynthesis and that a reduction of the monomeric carbon chain lengths leads to increased rates of peridermal transpiration.

Show MeSH
Periderm water permeances of StKCS6-RNAi and wild-type tubers from freshly-harvested and 21-d-stored tubers. After 21 d storage, periderms improve the water barrier properties showing a decreased water permeance (P; m s−1). At this stage, the StKCS6-silenced periderms have a 1.54-fold increase on water permeance when compared to wild-type (t test; **, P < 0.01). Data represent the mean and error bars the 95% confidence intervals for the periderms from wild-type freshly-harvested (n=11) and 21-d-stored (n=14) and for the StKCS6-RNAi freshly-harvested (line 5 n=7; line 34 n=11) and 21-d-stored (line 5 n=9; line 34 n=11) tubers.
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fig5: Periderm water permeances of StKCS6-RNAi and wild-type tubers from freshly-harvested and 21-d-stored tubers. After 21 d storage, periderms improve the water barrier properties showing a decreased water permeance (P; m s−1). At this stage, the StKCS6-silenced periderms have a 1.54-fold increase on water permeance when compared to wild-type (t test; **, P < 0.01). Data represent the mean and error bars the 95% confidence intervals for the periderms from wild-type freshly-harvested (n=11) and 21-d-stored (n=14) and for the StKCS6-RNAi freshly-harvested (line 5 n=7; line 34 n=11) and 21-d-stored (line 5 n=9; line 34 n=11) tubers.

Mentions: Periderms isolated from 21-d-stored tubers showed transpiration values significantly lower than those of periderms from freshly-harvested tubers (Fig. 5). As regards StKCS6-silenced lines, peridermal transpiration was higher than wild-type either at 21 d storage as at 0 d storage (Fig. 5). The transpiration increase was statistically significant for the two independent silenced lines analysed (5 and 34) at 21 d storage but only for line 5 at 0 d storage.


Silencing of StKCS6 in potato periderm leads to reduced chain lengths of suberin and wax compounds and increased peridermal transpiration.

Serra O, Soler M, Hohn C, Franke R, Schreiber L, Prat S, Molinas M, Figueras M - J. Exp. Bot. (2008)

Periderm water permeances of StKCS6-RNAi and wild-type tubers from freshly-harvested and 21-d-stored tubers. After 21 d storage, periderms improve the water barrier properties showing a decreased water permeance (P; m s−1). At this stage, the StKCS6-silenced periderms have a 1.54-fold increase on water permeance when compared to wild-type (t test; **, P < 0.01). Data represent the mean and error bars the 95% confidence intervals for the periderms from wild-type freshly-harvested (n=11) and 21-d-stored (n=14) and for the StKCS6-RNAi freshly-harvested (line 5 n=7; line 34 n=11) and 21-d-stored (line 5 n=9; line 34 n=11) tubers.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2651458&req=5

fig5: Periderm water permeances of StKCS6-RNAi and wild-type tubers from freshly-harvested and 21-d-stored tubers. After 21 d storage, periderms improve the water barrier properties showing a decreased water permeance (P; m s−1). At this stage, the StKCS6-silenced periderms have a 1.54-fold increase on water permeance when compared to wild-type (t test; **, P < 0.01). Data represent the mean and error bars the 95% confidence intervals for the periderms from wild-type freshly-harvested (n=11) and 21-d-stored (n=14) and for the StKCS6-RNAi freshly-harvested (line 5 n=7; line 34 n=11) and 21-d-stored (line 5 n=9; line 34 n=11) tubers.
Mentions: Periderms isolated from 21-d-stored tubers showed transpiration values significantly lower than those of periderms from freshly-harvested tubers (Fig. 5). As regards StKCS6-silenced lines, peridermal transpiration was higher than wild-type either at 21 d storage as at 0 d storage (Fig. 5). The transpiration increase was statistically significant for the two independent silenced lines analysed (5 and 34) at 21 d storage but only for line 5 at 0 d storage.

Bottom Line: All compounds with chain lengths of C(28) and higher were significantly reduced in silenced periderms, whereas compounds with chain lengths of C(26) and lower accumulated.As a result, peridermal transpiration of the silenced lines was about 1.5-times higher than that of the wild type.Our results convincingly show that StKCS6 is involved in both suberin and wax biosynthesis and that a reduction of the monomeric carbon chain lengths leads to increased rates of peridermal transpiration.

View Article: PubMed Central - PubMed

Affiliation: Laboratori del Suro, Departament de Biologia, Facultat de Ciències, Universitat de Girona, Campus Montilivi s/n, E-17071 Girona, Spain.

ABSTRACT
Very long chain aliphatic compounds occur in the suberin polymer and associated wax. Up to now only few genes involved in suberin biosynthesis have been identified. This is a report on the isolation of a potato (Solanum tuberosum) 3-ketoacyl-CoA synthase (KCS) gene and the study of its molecular and physiological relevance by means of a reverse genetic approach. This gene, called StKCS6, was stably silenced by RNA interference (RNAi) in potato. Analysis of the chemical composition of silenced potato tuber periderms indicated that StKCS6 down-regulation has a significant and fairly specific effect on the chain length distribution of very long-chain fatty acids (VLCFAs) and derivatives, occurring in the suberin polymer and peridermal wax. All compounds with chain lengths of C(28) and higher were significantly reduced in silenced periderms, whereas compounds with chain lengths of C(26) and lower accumulated. Thus, StKCS6 is preferentially involved in the formation of suberin and wax lipidic monomers with chain lengths of C(28) and higher. As a result, peridermal transpiration of the silenced lines was about 1.5-times higher than that of the wild type. Our results convincingly show that StKCS6 is involved in both suberin and wax biosynthesis and that a reduction of the monomeric carbon chain lengths leads to increased rates of peridermal transpiration.

Show MeSH