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Molecular responses of Lotus japonicus to parasitism by the compatible species Orobanche aegyptiaca and the incompatible species Striga hermonthica.

Hiraoka Y, Ueda H, Sugimoto Y - J. Exp. Bot. (2008)

Bottom Line: Nodulation-related genes were almost exclusively found among the Orobanche-induced genes.Four genes, including putative trypsin protease inhibitor genes, exhibited systemic up-regulation in the host plant parasitized by O. aegyptiaca.On the other hand, S. hermonthica attachment did not induce systemic gene expression.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Agricultural Science, Kobe University, Rokkodai, Nada, 657-8501 Kobe, Japan.

ABSTRACT
Lotus japonicus genes responsive to parasitism by the compatible species Orobanche aegyptiaca and the incompatible species Striga hermonthica were isolated by using the suppression subtractive hybridization (SSH) strategy. O. aegyptiaca and S. hermonthica parasitism specifically induced the expression of genes involved in jasmonic acid (JA) biosynthesis and phytoalexin biosynthesis, respectively. Nodulation-related genes were almost exclusively found among the Orobanche-induced genes. Temporal gene expression analyses revealed that 19 out of the 48 Orobanche-induced genes and 5 out of the 48 Striga-induced genes were up-regulated at 1 dai. Four genes, including putative trypsin protease inhibitor genes, exhibited systemic up-regulation in the host plant parasitized by O. aegyptiaca. On the other hand, S. hermonthica attachment did not induce systemic gene expression.

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Related in: MedlinePlus

Local expression (A) and systemic expression (B) in Lotus japonicus roots, and systemic expression (C) in L. japonicus leaves of the four Orobanche-induced genes. The x-axis indicates the clone number and the days after inoculation of each gene. The y-axis indicates fold expression induction (log scale). The error bars represent the SD of the inductions.
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fig5: Local expression (A) and systemic expression (B) in Lotus japonicus roots, and systemic expression (C) in L. japonicus leaves of the four Orobanche-induced genes. The x-axis indicates the clone number and the days after inoculation of each gene. The y-axis indicates fold expression induction (log scale). The error bars represent the SD of the inductions.

Mentions: Genes were selected that exhibited greater than 8-fold up-regulation at either time point after the inoculation of O. aegyptiaca or S. hermonthica (Figs 4A, 5A), and their systemic expression was analysed (Figs 4B, 5B, 5C). Among 16 Orobanche-induced genes, four genes, namely, LjOa9, LjOa116-3s, LjOa169, and LjOa147-2 exhibited greater than 8-fold up-regulation at 10 dai (Fig. 5B). The expression of these four genes in the leaves was also analysed, and a 10-fold up-regulation of LjOa9 expression was detected at 10 dai (Fig. 5C). Similarly, the systemic expression of 14 genes selected from among the Striga-induced genes was analysed in the uninoculated roots at 1, 2, and 6 dai (Fig. 4B). However, no gene exhibited significant up-regulation (Fig. 4B).


Molecular responses of Lotus japonicus to parasitism by the compatible species Orobanche aegyptiaca and the incompatible species Striga hermonthica.

Hiraoka Y, Ueda H, Sugimoto Y - J. Exp. Bot. (2008)

Local expression (A) and systemic expression (B) in Lotus japonicus roots, and systemic expression (C) in L. japonicus leaves of the four Orobanche-induced genes. The x-axis indicates the clone number and the days after inoculation of each gene. The y-axis indicates fold expression induction (log scale). The error bars represent the SD of the inductions.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2651452&req=5

fig5: Local expression (A) and systemic expression (B) in Lotus japonicus roots, and systemic expression (C) in L. japonicus leaves of the four Orobanche-induced genes. The x-axis indicates the clone number and the days after inoculation of each gene. The y-axis indicates fold expression induction (log scale). The error bars represent the SD of the inductions.
Mentions: Genes were selected that exhibited greater than 8-fold up-regulation at either time point after the inoculation of O. aegyptiaca or S. hermonthica (Figs 4A, 5A), and their systemic expression was analysed (Figs 4B, 5B, 5C). Among 16 Orobanche-induced genes, four genes, namely, LjOa9, LjOa116-3s, LjOa169, and LjOa147-2 exhibited greater than 8-fold up-regulation at 10 dai (Fig. 5B). The expression of these four genes in the leaves was also analysed, and a 10-fold up-regulation of LjOa9 expression was detected at 10 dai (Fig. 5C). Similarly, the systemic expression of 14 genes selected from among the Striga-induced genes was analysed in the uninoculated roots at 1, 2, and 6 dai (Fig. 4B). However, no gene exhibited significant up-regulation (Fig. 4B).

Bottom Line: Nodulation-related genes were almost exclusively found among the Orobanche-induced genes.Four genes, including putative trypsin protease inhibitor genes, exhibited systemic up-regulation in the host plant parasitized by O. aegyptiaca.On the other hand, S. hermonthica attachment did not induce systemic gene expression.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Agricultural Science, Kobe University, Rokkodai, Nada, 657-8501 Kobe, Japan.

ABSTRACT
Lotus japonicus genes responsive to parasitism by the compatible species Orobanche aegyptiaca and the incompatible species Striga hermonthica were isolated by using the suppression subtractive hybridization (SSH) strategy. O. aegyptiaca and S. hermonthica parasitism specifically induced the expression of genes involved in jasmonic acid (JA) biosynthesis and phytoalexin biosynthesis, respectively. Nodulation-related genes were almost exclusively found among the Orobanche-induced genes. Temporal gene expression analyses revealed that 19 out of the 48 Orobanche-induced genes and 5 out of the 48 Striga-induced genes were up-regulated at 1 dai. Four genes, including putative trypsin protease inhibitor genes, exhibited systemic up-regulation in the host plant parasitized by O. aegyptiaca. On the other hand, S. hermonthica attachment did not induce systemic gene expression.

Show MeSH
Related in: MedlinePlus