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Molecular responses of Lotus japonicus to parasitism by the compatible species Orobanche aegyptiaca and the incompatible species Striga hermonthica.

Hiraoka Y, Ueda H, Sugimoto Y - J. Exp. Bot. (2008)

Bottom Line: Nodulation-related genes were almost exclusively found among the Orobanche-induced genes.Four genes, including putative trypsin protease inhibitor genes, exhibited systemic up-regulation in the host plant parasitized by O. aegyptiaca.On the other hand, S. hermonthica attachment did not induce systemic gene expression.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Agricultural Science, Kobe University, Rokkodai, Nada, 657-8501 Kobe, Japan.

ABSTRACT
Lotus japonicus genes responsive to parasitism by the compatible species Orobanche aegyptiaca and the incompatible species Striga hermonthica were isolated by using the suppression subtractive hybridization (SSH) strategy. O. aegyptiaca and S. hermonthica parasitism specifically induced the expression of genes involved in jasmonic acid (JA) biosynthesis and phytoalexin biosynthesis, respectively. Nodulation-related genes were almost exclusively found among the Orobanche-induced genes. Temporal gene expression analyses revealed that 19 out of the 48 Orobanche-induced genes and 5 out of the 48 Striga-induced genes were up-regulated at 1 dai. Four genes, including putative trypsin protease inhibitor genes, exhibited systemic up-regulation in the host plant parasitized by O. aegyptiaca. On the other hand, S. hermonthica attachment did not induce systemic gene expression.

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Expression profiles of Lotus japonicus genes that were up-regulated in response to Striga hermonthica attachment as determined by performing quantitative RT-PCR. Clusters I, II, and III include genes that were up-regulated, constantly expressed, and down-regulated, respectively, at 1 d after S. hermonthica inoculation. The systemic expression of genes exhibiting greater than 8-fold up-regulation at either time point after S. hermonthica inoculation onto the L. japonicus roots was analysed. Tile colours indicate the relative fold expression: green corresponds to less than 0.125-fold down-regulation; red, greater than 8-fold up-regulation; and yellow, constant expression (bottom-most panel) on comparison of the inoculated and uninoculated samples (bottom-most panel).
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fig3: Expression profiles of Lotus japonicus genes that were up-regulated in response to Striga hermonthica attachment as determined by performing quantitative RT-PCR. Clusters I, II, and III include genes that were up-regulated, constantly expressed, and down-regulated, respectively, at 1 d after S. hermonthica inoculation. The systemic expression of genes exhibiting greater than 8-fold up-regulation at either time point after S. hermonthica inoculation onto the L. japonicus roots was analysed. Tile colours indicate the relative fold expression: green corresponds to less than 0.125-fold down-regulation; red, greater than 8-fold up-regulation; and yellow, constant expression (bottom-most panel) on comparison of the inoculated and uninoculated samples (bottom-most panel).

Mentions: Temporal changes in the expression levels of the Orobanche-induced genes in the L. japonicus roots were evaluated at 1, 2, and 10 dai. Similarly, the expression levels of the Striga-induced genes in the roots were evaluated at 1, 2, and 6 dai. On the basis of the expression at 1 dai, all the genes were classified into three clusters as shown in Figs 2 and 3. Clusters I, II, and III comprised genes that exhibited up-regulation, constant expression, and down-regulation, respectively, at 1 dai. Of the Orobanche-induced genes, 19, 26, and 3 genes were classified into clusters I, II, and III, respectively (Fig. 2). On the other hand, 6, 33, and 9 of the Striga-induced genes were classified into these respective clusters (Fig. 3). Of the genes in cluster I, the expression levels of 11 Orobanche-induced genes and three Striga-induced genes were similar to those in uninoculated roots at 2 dai (Figs 2, 3), and those in LjOa116-3s and LjOa25 exhibited transient down-regulation at 2 dai (Fig. 2). In the clusters II and III, the expression of most Orobanche- and Striga-induced genes was up-regulated only at 10 and 6 dai, respectively (Figs 2, 3).


Molecular responses of Lotus japonicus to parasitism by the compatible species Orobanche aegyptiaca and the incompatible species Striga hermonthica.

Hiraoka Y, Ueda H, Sugimoto Y - J. Exp. Bot. (2008)

Expression profiles of Lotus japonicus genes that were up-regulated in response to Striga hermonthica attachment as determined by performing quantitative RT-PCR. Clusters I, II, and III include genes that were up-regulated, constantly expressed, and down-regulated, respectively, at 1 d after S. hermonthica inoculation. The systemic expression of genes exhibiting greater than 8-fold up-regulation at either time point after S. hermonthica inoculation onto the L. japonicus roots was analysed. Tile colours indicate the relative fold expression: green corresponds to less than 0.125-fold down-regulation; red, greater than 8-fold up-regulation; and yellow, constant expression (bottom-most panel) on comparison of the inoculated and uninoculated samples (bottom-most panel).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2651452&req=5

fig3: Expression profiles of Lotus japonicus genes that were up-regulated in response to Striga hermonthica attachment as determined by performing quantitative RT-PCR. Clusters I, II, and III include genes that were up-regulated, constantly expressed, and down-regulated, respectively, at 1 d after S. hermonthica inoculation. The systemic expression of genes exhibiting greater than 8-fold up-regulation at either time point after S. hermonthica inoculation onto the L. japonicus roots was analysed. Tile colours indicate the relative fold expression: green corresponds to less than 0.125-fold down-regulation; red, greater than 8-fold up-regulation; and yellow, constant expression (bottom-most panel) on comparison of the inoculated and uninoculated samples (bottom-most panel).
Mentions: Temporal changes in the expression levels of the Orobanche-induced genes in the L. japonicus roots were evaluated at 1, 2, and 10 dai. Similarly, the expression levels of the Striga-induced genes in the roots were evaluated at 1, 2, and 6 dai. On the basis of the expression at 1 dai, all the genes were classified into three clusters as shown in Figs 2 and 3. Clusters I, II, and III comprised genes that exhibited up-regulation, constant expression, and down-regulation, respectively, at 1 dai. Of the Orobanche-induced genes, 19, 26, and 3 genes were classified into clusters I, II, and III, respectively (Fig. 2). On the other hand, 6, 33, and 9 of the Striga-induced genes were classified into these respective clusters (Fig. 3). Of the genes in cluster I, the expression levels of 11 Orobanche-induced genes and three Striga-induced genes were similar to those in uninoculated roots at 2 dai (Figs 2, 3), and those in LjOa116-3s and LjOa25 exhibited transient down-regulation at 2 dai (Fig. 2). In the clusters II and III, the expression of most Orobanche- and Striga-induced genes was up-regulated only at 10 and 6 dai, respectively (Figs 2, 3).

Bottom Line: Nodulation-related genes were almost exclusively found among the Orobanche-induced genes.Four genes, including putative trypsin protease inhibitor genes, exhibited systemic up-regulation in the host plant parasitized by O. aegyptiaca.On the other hand, S. hermonthica attachment did not induce systemic gene expression.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Agricultural Science, Kobe University, Rokkodai, Nada, 657-8501 Kobe, Japan.

ABSTRACT
Lotus japonicus genes responsive to parasitism by the compatible species Orobanche aegyptiaca and the incompatible species Striga hermonthica were isolated by using the suppression subtractive hybridization (SSH) strategy. O. aegyptiaca and S. hermonthica parasitism specifically induced the expression of genes involved in jasmonic acid (JA) biosynthesis and phytoalexin biosynthesis, respectively. Nodulation-related genes were almost exclusively found among the Orobanche-induced genes. Temporal gene expression analyses revealed that 19 out of the 48 Orobanche-induced genes and 5 out of the 48 Striga-induced genes were up-regulated at 1 dai. Four genes, including putative trypsin protease inhibitor genes, exhibited systemic up-regulation in the host plant parasitized by O. aegyptiaca. On the other hand, S. hermonthica attachment did not induce systemic gene expression.

Show MeSH
Related in: MedlinePlus