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Molecular responses of Lotus japonicus to parasitism by the compatible species Orobanche aegyptiaca and the incompatible species Striga hermonthica.

Hiraoka Y, Ueda H, Sugimoto Y - J. Exp. Bot. (2008)

Bottom Line: Nodulation-related genes were almost exclusively found among the Orobanche-induced genes.Four genes, including putative trypsin protease inhibitor genes, exhibited systemic up-regulation in the host plant parasitized by O. aegyptiaca.On the other hand, S. hermonthica attachment did not induce systemic gene expression.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Agricultural Science, Kobe University, Rokkodai, Nada, 657-8501 Kobe, Japan.

ABSTRACT
Lotus japonicus genes responsive to parasitism by the compatible species Orobanche aegyptiaca and the incompatible species Striga hermonthica were isolated by using the suppression subtractive hybridization (SSH) strategy. O. aegyptiaca and S. hermonthica parasitism specifically induced the expression of genes involved in jasmonic acid (JA) biosynthesis and phytoalexin biosynthesis, respectively. Nodulation-related genes were almost exclusively found among the Orobanche-induced genes. Temporal gene expression analyses revealed that 19 out of the 48 Orobanche-induced genes and 5 out of the 48 Striga-induced genes were up-regulated at 1 dai. Four genes, including putative trypsin protease inhibitor genes, exhibited systemic up-regulation in the host plant parasitized by O. aegyptiaca. On the other hand, S. hermonthica attachment did not induce systemic gene expression.

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Related in: MedlinePlus

Diagram of the modified split-root assembly. (A), square Petri dishes; (B), glass fibre papers; (C), rockwool; (D), Orobanche aegyptiaca or Striga hermonthica inoculated on one half of the Lotus japonicus roots.
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fig1: Diagram of the modified split-root assembly. (A), square Petri dishes; (B), glass fibre papers; (C), rockwool; (D), Orobanche aegyptiaca or Striga hermonthica inoculated on one half of the Lotus japonicus roots.

Mentions: For analyses of the systemic gene expression triggered in response to O. aegyptiaca and S. hermonthica parasitism, the split-root system as described by Kosslak and Bohlool (1984) was employed with some modifications. A modified split-root system was developed using two square Petri dishes (height, 14.4 cm; width, 10.4 cm; thickness, 1.6 cm), filled with rockwool, and overlaid with glass fibre paper. This system was carefully designed to prevent any exchange of material between the dishes (Fig. 1).


Molecular responses of Lotus japonicus to parasitism by the compatible species Orobanche aegyptiaca and the incompatible species Striga hermonthica.

Hiraoka Y, Ueda H, Sugimoto Y - J. Exp. Bot. (2008)

Diagram of the modified split-root assembly. (A), square Petri dishes; (B), glass fibre papers; (C), rockwool; (D), Orobanche aegyptiaca or Striga hermonthica inoculated on one half of the Lotus japonicus roots.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2651452&req=5

fig1: Diagram of the modified split-root assembly. (A), square Petri dishes; (B), glass fibre papers; (C), rockwool; (D), Orobanche aegyptiaca or Striga hermonthica inoculated on one half of the Lotus japonicus roots.
Mentions: For analyses of the systemic gene expression triggered in response to O. aegyptiaca and S. hermonthica parasitism, the split-root system as described by Kosslak and Bohlool (1984) was employed with some modifications. A modified split-root system was developed using two square Petri dishes (height, 14.4 cm; width, 10.4 cm; thickness, 1.6 cm), filled with rockwool, and overlaid with glass fibre paper. This system was carefully designed to prevent any exchange of material between the dishes (Fig. 1).

Bottom Line: Nodulation-related genes were almost exclusively found among the Orobanche-induced genes.Four genes, including putative trypsin protease inhibitor genes, exhibited systemic up-regulation in the host plant parasitized by O. aegyptiaca.On the other hand, S. hermonthica attachment did not induce systemic gene expression.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Agricultural Science, Kobe University, Rokkodai, Nada, 657-8501 Kobe, Japan.

ABSTRACT
Lotus japonicus genes responsive to parasitism by the compatible species Orobanche aegyptiaca and the incompatible species Striga hermonthica were isolated by using the suppression subtractive hybridization (SSH) strategy. O. aegyptiaca and S. hermonthica parasitism specifically induced the expression of genes involved in jasmonic acid (JA) biosynthesis and phytoalexin biosynthesis, respectively. Nodulation-related genes were almost exclusively found among the Orobanche-induced genes. Temporal gene expression analyses revealed that 19 out of the 48 Orobanche-induced genes and 5 out of the 48 Striga-induced genes were up-regulated at 1 dai. Four genes, including putative trypsin protease inhibitor genes, exhibited systemic up-regulation in the host plant parasitized by O. aegyptiaca. On the other hand, S. hermonthica attachment did not induce systemic gene expression.

Show MeSH
Related in: MedlinePlus