Limits...
RNA silencing is required for Arabidopsis defence against Verticillium wilt disease.

Ellendorff U, Fradin EF, de Jonge R, Thomma BP - J. Exp. Bot. (2008)

Bottom Line: Recently, RNA silencing has been found to play a role in defence against bacterial plant pathogens in Arabidopsis through modulating host defence responses.Several components of RNA silencing pathways were tested, of which many were found to affect Verticillium defence.Since the observed differences in Verticillium susceptibility cannot be explained by notable differences in root architecture, it is speculated that the gene silencing mechanisms affect regulation of Verticillium-specific defence responses.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Phytopathology, Wageningen University, Binnenhaven 5, 6709 PD Wageningen, The Netherlands.

ABSTRACT
RNA silencing is a conserved mechanism in eukaryotes that plays an important role in various biological processes including regulation of gene expression. RNA silencing also plays a role in genome stability and protects plants against invading nucleic acids such as transgenes and viruses. Recently, RNA silencing has been found to play a role in defence against bacterial plant pathogens in Arabidopsis through modulating host defence responses. In this study, it is shown that gene silencing plays a role in plant defence against multicellular microbial pathogens; vascular fungi belonging to the Verticillium genus. Several components of RNA silencing pathways were tested, of which many were found to affect Verticillium defence. Remarkably, no altered defence towards other fungal pathogens that include Alternaria brassicicola, Botrytis cinerea, and Plectosphaerella cucumerina, but also the vascular pathogen Fusarium oxysporum, was recorded. Since the observed differences in Verticillium susceptibility cannot be explained by notable differences in root architecture, it is speculated that the gene silencing mechanisms affect regulation of Verticillium-specific defence responses.

Show MeSH

Related in: MedlinePlus

Typical root architecture of in vitro-grown Arabidopsis gene silencing mutants. Roots were grown on vertically oriented MS plates and pictures were taken 10 d after sowing. (This figure is available in colour at JXB online.)
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC2651451&req=5

fig4: Typical root architecture of in vitro-grown Arabidopsis gene silencing mutants. Roots were grown on vertically oriented MS plates and pictures were taken 10 d after sowing. (This figure is available in colour at JXB online.)

Mentions: Being a root pathogen, differences in Verticillium susceptibility of the different mutants may be explained by differences in root architecture, the tissues that are inoculated. Although no obvious differences in root architecture were observed during uprooting and inoculation of the mutants, except for the ago mutants that had developed shorter roots, root development and architecture was assessed upon in vitro growth on MS medium. However, apart from rather slight growth differences, no notable differences in root development and architecture were observed for the RNA-silencing mutants that correlated with the differences in Verticillium susceptibility (Fig. 4). For all mutants, development of the primary, dominant, root was followed by production of lateral roots in a later stage.


RNA silencing is required for Arabidopsis defence against Verticillium wilt disease.

Ellendorff U, Fradin EF, de Jonge R, Thomma BP - J. Exp. Bot. (2008)

Typical root architecture of in vitro-grown Arabidopsis gene silencing mutants. Roots were grown on vertically oriented MS plates and pictures were taken 10 d after sowing. (This figure is available in colour at JXB online.)
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2651451&req=5

fig4: Typical root architecture of in vitro-grown Arabidopsis gene silencing mutants. Roots were grown on vertically oriented MS plates and pictures were taken 10 d after sowing. (This figure is available in colour at JXB online.)
Mentions: Being a root pathogen, differences in Verticillium susceptibility of the different mutants may be explained by differences in root architecture, the tissues that are inoculated. Although no obvious differences in root architecture were observed during uprooting and inoculation of the mutants, except for the ago mutants that had developed shorter roots, root development and architecture was assessed upon in vitro growth on MS medium. However, apart from rather slight growth differences, no notable differences in root development and architecture were observed for the RNA-silencing mutants that correlated with the differences in Verticillium susceptibility (Fig. 4). For all mutants, development of the primary, dominant, root was followed by production of lateral roots in a later stage.

Bottom Line: Recently, RNA silencing has been found to play a role in defence against bacterial plant pathogens in Arabidopsis through modulating host defence responses.Several components of RNA silencing pathways were tested, of which many were found to affect Verticillium defence.Since the observed differences in Verticillium susceptibility cannot be explained by notable differences in root architecture, it is speculated that the gene silencing mechanisms affect regulation of Verticillium-specific defence responses.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Phytopathology, Wageningen University, Binnenhaven 5, 6709 PD Wageningen, The Netherlands.

ABSTRACT
RNA silencing is a conserved mechanism in eukaryotes that plays an important role in various biological processes including regulation of gene expression. RNA silencing also plays a role in genome stability and protects plants against invading nucleic acids such as transgenes and viruses. Recently, RNA silencing has been found to play a role in defence against bacterial plant pathogens in Arabidopsis through modulating host defence responses. In this study, it is shown that gene silencing plays a role in plant defence against multicellular microbial pathogens; vascular fungi belonging to the Verticillium genus. Several components of RNA silencing pathways were tested, of which many were found to affect Verticillium defence. Remarkably, no altered defence towards other fungal pathogens that include Alternaria brassicicola, Botrytis cinerea, and Plectosphaerella cucumerina, but also the vascular pathogen Fusarium oxysporum, was recorded. Since the observed differences in Verticillium susceptibility cannot be explained by notable differences in root architecture, it is speculated that the gene silencing mechanisms affect regulation of Verticillium-specific defence responses.

Show MeSH
Related in: MedlinePlus