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The association of HBV core promoter double mutations (A1762T and G1764A) with viral load differs between HBeAg positive and anti-HBe positive individuals: a longitudinal analysis.

Fang ZL, Sabin CA, Dong BQ, Wei SC, Chen QY, Fang KX, Yang JY, Wang XY, Harrison TJ - J. Hepatol. (2008)

Bottom Line: We aim to determine the association after controlling for HBeAg - a strong confounding factor.In multivariable analysis of the cross-sectional data, BCP double mutations are significantly associated with lower viral loads in HBeAg positive subjects but no difference was found in anti-HBe positive subjects.Analysis of the longitudinal data yielded similar results to the cross-sectional data.

View Article: PubMed Central - PubMed

Affiliation: Division of Medicine, UCL Medical School, Windeyer Building, Cleveland Street, London W1T 4JF, UK.

ABSTRACT

Background/aims: Although there have been a few reports regarding the effect of basal core promoter (BCP) double mutations (A1762T and G1764A) on hepatitis B viral loads, the association remains uncertain. We aim to determine the association after controlling for HBeAg - a strong confounding factor.

Methods: We selected randomly 190 individuals from a Chinese cohort of 2258 subjects for cross-sectional analysis and 56 of the 190 for longitudinal analysis of viral loads.

Results: In multivariable analysis of the cross-sectional data, BCP double mutations are significantly associated with lower viral loads in HBeAg positive subjects but no difference was found in anti-HBe positive subjects. Triple mutations at nucleotide (nt) 1753, 1762 and 1764 and mutations between nt 1809 and 1817, precore stop mutation (nt 1896) and genotype are not associated with viral loads in either HBeAg or anti-HBe positive subjects. Analysis of the longitudinal data yielded similar results to the cross-sectional data. Viral loads differ significantly between individuals infected with wild-type and BCP double mutations prior to HBeAg seroconversion but this difference is lost after seroconversion.

Conclusions: BCP double mutations are associated with lower viral loads in HBeAg positive individuals but have no effect on the viral loads of anti-HBe positive individuals.

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Related in: MedlinePlus

Longitudinal analysis of viral loads according to BCP double mutations and HBeAg status. The box plot shows median values, upper and lower quartiles and the largest and smallest observations.
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fig1: Longitudinal analysis of viral loads according to BCP double mutations and HBeAg status. The box plot shows median values, upper and lower quartiles and the largest and smallest observations.

Mentions: Of those who were HBeAg positive, 13 subjects with BCP double mutations and 9 without were included in the longitudinal analysis; of those who were HBeAg negative, 17 and 19 subjects were in the two subgroups, respectively. The medians of the viral loads for both the BCP wild-type and BCP double mutations subgroups are higher in the HBeAg positive group than the anti-HBe positive group over 3 years. In the HBeAg positive group, viral loads were significantly lower in the BCP double mutations subgroup than the wild-type subgroup at months 0, 12, 24 and 36 (p = 0.02, 0.001, 0.04 and 0.008 at each timepoint, respectively). However, in the anti-HBe positive group, there were no significant differences in the viral loads of the BCP double mutations and BCP wild-type subgroups at the same timepoints (p = 0.61, 0.25, 0.61 and 0.12, respectively; Table 3 and Fig. 1).


The association of HBV core promoter double mutations (A1762T and G1764A) with viral load differs between HBeAg positive and anti-HBe positive individuals: a longitudinal analysis.

Fang ZL, Sabin CA, Dong BQ, Wei SC, Chen QY, Fang KX, Yang JY, Wang XY, Harrison TJ - J. Hepatol. (2008)

Longitudinal analysis of viral loads according to BCP double mutations and HBeAg status. The box plot shows median values, upper and lower quartiles and the largest and smallest observations.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2648871&req=5

fig1: Longitudinal analysis of viral loads according to BCP double mutations and HBeAg status. The box plot shows median values, upper and lower quartiles and the largest and smallest observations.
Mentions: Of those who were HBeAg positive, 13 subjects with BCP double mutations and 9 without were included in the longitudinal analysis; of those who were HBeAg negative, 17 and 19 subjects were in the two subgroups, respectively. The medians of the viral loads for both the BCP wild-type and BCP double mutations subgroups are higher in the HBeAg positive group than the anti-HBe positive group over 3 years. In the HBeAg positive group, viral loads were significantly lower in the BCP double mutations subgroup than the wild-type subgroup at months 0, 12, 24 and 36 (p = 0.02, 0.001, 0.04 and 0.008 at each timepoint, respectively). However, in the anti-HBe positive group, there were no significant differences in the viral loads of the BCP double mutations and BCP wild-type subgroups at the same timepoints (p = 0.61, 0.25, 0.61 and 0.12, respectively; Table 3 and Fig. 1).

Bottom Line: We aim to determine the association after controlling for HBeAg - a strong confounding factor.In multivariable analysis of the cross-sectional data, BCP double mutations are significantly associated with lower viral loads in HBeAg positive subjects but no difference was found in anti-HBe positive subjects.Analysis of the longitudinal data yielded similar results to the cross-sectional data.

View Article: PubMed Central - PubMed

Affiliation: Division of Medicine, UCL Medical School, Windeyer Building, Cleveland Street, London W1T 4JF, UK.

ABSTRACT

Background/aims: Although there have been a few reports regarding the effect of basal core promoter (BCP) double mutations (A1762T and G1764A) on hepatitis B viral loads, the association remains uncertain. We aim to determine the association after controlling for HBeAg - a strong confounding factor.

Methods: We selected randomly 190 individuals from a Chinese cohort of 2258 subjects for cross-sectional analysis and 56 of the 190 for longitudinal analysis of viral loads.

Results: In multivariable analysis of the cross-sectional data, BCP double mutations are significantly associated with lower viral loads in HBeAg positive subjects but no difference was found in anti-HBe positive subjects. Triple mutations at nucleotide (nt) 1753, 1762 and 1764 and mutations between nt 1809 and 1817, precore stop mutation (nt 1896) and genotype are not associated with viral loads in either HBeAg or anti-HBe positive subjects. Analysis of the longitudinal data yielded similar results to the cross-sectional data. Viral loads differ significantly between individuals infected with wild-type and BCP double mutations prior to HBeAg seroconversion but this difference is lost after seroconversion.

Conclusions: BCP double mutations are associated with lower viral loads in HBeAg positive individuals but have no effect on the viral loads of anti-HBe positive individuals.

Show MeSH
Related in: MedlinePlus