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Maize genome in motion.

Walbot V - Genome Biol. (2008)

Bottom Line: A report on the Maize Genetics Conference held in Washington DC, USA, 27 February-1 March, 2008.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biology, Serra Mall, Stanford University, Stanford, CA 94305-5020, USA. Walbot@stanford.edu

ABSTRACT
A report on the Maize Genetics Conference held in Washington DC, USA, 27 February-1 March, 2008.

Show MeSH
Retrotransposons collectively comprise 76% of the maize genome. In this preliminary analysis of the draft genome sequence by Josh Stein (MGSC, Cold Spring Harbor Laboratory, Cold Spring Harbor, USA) it is clear that just the gypsy family huck element plus the copia relative ji make up nearly one-quarter of the genome - about 600 Mb. Image courtesy of Richard Wilson and Josh Stein.
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Figure 1: Retrotransposons collectively comprise 76% of the maize genome. In this preliminary analysis of the draft genome sequence by Josh Stein (MGSC, Cold Spring Harbor Laboratory, Cold Spring Harbor, USA) it is clear that just the gypsy family huck element plus the copia relative ji make up nearly one-quarter of the genome - about 600 Mb. Image courtesy of Richard Wilson and Josh Stein.

Mentions: The draft genome of the maize inbred line B73 is the template for analyzing genome fluidity and epigenetic regulation on a global scale. Rick Wilson (Washington University School of Medicine, St Louis, USA), leader of the Maize Genome Sequencing Consortium (MGSC), reported that maize could be the last genome sequenced using a complete bacterial artificial chromosome (BAC) tiling path. He commented that successful sequencing may have required this method, however, as the maize genome is 78% repetitive DNA, mostly composed of 11 types of retro-transposons (Figure 1). A typical 150-kB BAC contains one or a few compact genes (similar in size to those of other plants but 10-fold shorter than in mammals) and many nested retroelements. Given funding of less than US$30 million, about 1% the funding for the Human Genome Project, the strategy is to finish genes to very high quality but leave the retrotransposon mess partially unassembled. As transcription, recombination and DNA transposon insertion are all centered on genes, the four or so gaps per BAC in the retrotransposon graveyard between genes should be almost invisible during genetic and molecular analyses.


Maize genome in motion.

Walbot V - Genome Biol. (2008)

Retrotransposons collectively comprise 76% of the maize genome. In this preliminary analysis of the draft genome sequence by Josh Stein (MGSC, Cold Spring Harbor Laboratory, Cold Spring Harbor, USA) it is clear that just the gypsy family huck element plus the copia relative ji make up nearly one-quarter of the genome - about 600 Mb. Image courtesy of Richard Wilson and Josh Stein.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2643931&req=5

Figure 1: Retrotransposons collectively comprise 76% of the maize genome. In this preliminary analysis of the draft genome sequence by Josh Stein (MGSC, Cold Spring Harbor Laboratory, Cold Spring Harbor, USA) it is clear that just the gypsy family huck element plus the copia relative ji make up nearly one-quarter of the genome - about 600 Mb. Image courtesy of Richard Wilson and Josh Stein.
Mentions: The draft genome of the maize inbred line B73 is the template for analyzing genome fluidity and epigenetic regulation on a global scale. Rick Wilson (Washington University School of Medicine, St Louis, USA), leader of the Maize Genome Sequencing Consortium (MGSC), reported that maize could be the last genome sequenced using a complete bacterial artificial chromosome (BAC) tiling path. He commented that successful sequencing may have required this method, however, as the maize genome is 78% repetitive DNA, mostly composed of 11 types of retro-transposons (Figure 1). A typical 150-kB BAC contains one or a few compact genes (similar in size to those of other plants but 10-fold shorter than in mammals) and many nested retroelements. Given funding of less than US$30 million, about 1% the funding for the Human Genome Project, the strategy is to finish genes to very high quality but leave the retrotransposon mess partially unassembled. As transcription, recombination and DNA transposon insertion are all centered on genes, the four or so gaps per BAC in the retrotransposon graveyard between genes should be almost invisible during genetic and molecular analyses.

Bottom Line: A report on the Maize Genetics Conference held in Washington DC, USA, 27 February-1 March, 2008.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biology, Serra Mall, Stanford University, Stanford, CA 94305-5020, USA. Walbot@stanford.edu

ABSTRACT
A report on the Maize Genetics Conference held in Washington DC, USA, 27 February-1 March, 2008.

Show MeSH