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Endothelial progenitor cell number and colony-forming capacity in overweight and obese adults.

MacEneaney OJ, Kushner EJ, Van Guilder GP, Greiner JJ, Stauffer BL, DeSouza CA - Int J Obes (Lond) (2008)

Bottom Line: There were no differences in EPC number between the overweight and normal weight groups.EPC colony formation was significantly less in the obese (6+/-1) and overweight (4+/-1) compared with normal weight (9+/-2) adults.These results indicate that: (1) the number of circulating EPCs is lower in obese compared with overweight and normal weight adults; and (2) EPC colony-forming capacity is blunted in overweight and obese adults compared with normal weight adults.

View Article: PubMed Central - PubMed

Affiliation: Integrative Vascular Biology Laboratory, Department of Integrative Physiology, University of Colorado, Boulder, CO 80309, USA.

ABSTRACT

Objective: To investigate whether adiposity influences endothelial progenitor cell (EPC) number and colony-forming capacity.

Design: Cross-sectional study of normal weight, overweight and obese adult humans.

Participants: Sixty-seven sedentary adults (aged 45-65 years): 25 normal weight (body mass index (BMI) or=30 kg/m(2); 18 males/6 females). All participants were non-smokers and free of overt cardiometabolic disease.

Measurements: Peripheral blood samples were collected and circulating EPC number was assessed by flow cytometry. Putative EPCs were defined as CD45(-)/CD34(+)/VEGFR-2(+)/CD133(+) or CD45(-)/CD34(+) cells. EPC colony-forming capacity was measured in vitro using a colony-forming unit (CFU) assay.

Results: Number of circulating putative EPCs (either CD45(-)/CD34(+)/VEGFR-2(+)/CD133(+) or CD45(-)/CD34(+) cells) was lower (P<0.05) in obese (0.0007+/-0.0001%; 0.050+/-0.006%) compared with overweight (0.0016+/-0.0004%; 0.089+/-0.019%) and normal weight (0.0015+/-0.0003%; 0.082+/-0.008%) adults. There were no differences in EPC number between the overweight and normal weight groups. EPC colony formation was significantly less in the obese (6+/-1) and overweight (4+/-1) compared with normal weight (9+/-2) adults.

Conclusion: These results indicate that: (1) the number of circulating EPCs is lower in obese compared with overweight and normal weight adults; and (2) EPC colony-forming capacity is blunted in overweight and obese adults compared with normal weight adults. Impairments in EPC number and function may contribute to adiposity-related cardiovascular risk.

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Related in: MedlinePlus

Circulating levels of CD45−/CD34+/VEGFR-2+/CD133+ and CD45−/CD34+ cells in normal weight, overweight, and obese adults.Values are mean ± SE. *P< 0.05 vs. normal weight and overweight.
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Figure 1: Circulating levels of CD45−/CD34+/VEGFR-2+/CD133+ and CD45−/CD34+ cells in normal weight, overweight, and obese adults.Values are mean ± SE. *P< 0.05 vs. normal weight and overweight.

Mentions: The number of CD45−/CD34+/VEGFR-2+/CD133+ cells was ~50% lower (P<0.05) in the obese (0.0007 ± 0.0001 %) compared with the overweight (0.0016 ± 0.0004 %) and normal weight groups (0.0015 ± 0.0003 %) (Figure 1). Similarly, the number of CD45−/CD34+ cells was lower (P<0.05) in the obese (0.050±0.006%) compared with overweight (0.089±0.019%) and normal weight subjects (0.082±0.008%). There were no significant differences in either CD45−/CD34+/VEGFR-2+/CD133+ or CD45−/CD34+ cells between the overweight and normal-weight subjects. The capacity of EPCs to form colonies was lower (~40%; P<0.05) in both the overweight (4 ± 1) and obese (6 ± 1) groups versus the normal weight controls (9 ± 2) (Figure 2). There was no significant difference in EPC CFU number between the overweight and obese subjects.


Endothelial progenitor cell number and colony-forming capacity in overweight and obese adults.

MacEneaney OJ, Kushner EJ, Van Guilder GP, Greiner JJ, Stauffer BL, DeSouza CA - Int J Obes (Lond) (2008)

Circulating levels of CD45−/CD34+/VEGFR-2+/CD133+ and CD45−/CD34+ cells in normal weight, overweight, and obese adults.Values are mean ± SE. *P< 0.05 vs. normal weight and overweight.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2643316&req=5

Figure 1: Circulating levels of CD45−/CD34+/VEGFR-2+/CD133+ and CD45−/CD34+ cells in normal weight, overweight, and obese adults.Values are mean ± SE. *P< 0.05 vs. normal weight and overweight.
Mentions: The number of CD45−/CD34+/VEGFR-2+/CD133+ cells was ~50% lower (P<0.05) in the obese (0.0007 ± 0.0001 %) compared with the overweight (0.0016 ± 0.0004 %) and normal weight groups (0.0015 ± 0.0003 %) (Figure 1). Similarly, the number of CD45−/CD34+ cells was lower (P<0.05) in the obese (0.050±0.006%) compared with overweight (0.089±0.019%) and normal weight subjects (0.082±0.008%). There were no significant differences in either CD45−/CD34+/VEGFR-2+/CD133+ or CD45−/CD34+ cells between the overweight and normal-weight subjects. The capacity of EPCs to form colonies was lower (~40%; P<0.05) in both the overweight (4 ± 1) and obese (6 ± 1) groups versus the normal weight controls (9 ± 2) (Figure 2). There was no significant difference in EPC CFU number between the overweight and obese subjects.

Bottom Line: There were no differences in EPC number between the overweight and normal weight groups.EPC colony formation was significantly less in the obese (6+/-1) and overweight (4+/-1) compared with normal weight (9+/-2) adults.These results indicate that: (1) the number of circulating EPCs is lower in obese compared with overweight and normal weight adults; and (2) EPC colony-forming capacity is blunted in overweight and obese adults compared with normal weight adults.

View Article: PubMed Central - PubMed

Affiliation: Integrative Vascular Biology Laboratory, Department of Integrative Physiology, University of Colorado, Boulder, CO 80309, USA.

ABSTRACT

Objective: To investigate whether adiposity influences endothelial progenitor cell (EPC) number and colony-forming capacity.

Design: Cross-sectional study of normal weight, overweight and obese adult humans.

Participants: Sixty-seven sedentary adults (aged 45-65 years): 25 normal weight (body mass index (BMI) or=30 kg/m(2); 18 males/6 females). All participants were non-smokers and free of overt cardiometabolic disease.

Measurements: Peripheral blood samples were collected and circulating EPC number was assessed by flow cytometry. Putative EPCs were defined as CD45(-)/CD34(+)/VEGFR-2(+)/CD133(+) or CD45(-)/CD34(+) cells. EPC colony-forming capacity was measured in vitro using a colony-forming unit (CFU) assay.

Results: Number of circulating putative EPCs (either CD45(-)/CD34(+)/VEGFR-2(+)/CD133(+) or CD45(-)/CD34(+) cells) was lower (P<0.05) in obese (0.0007+/-0.0001%; 0.050+/-0.006%) compared with overweight (0.0016+/-0.0004%; 0.089+/-0.019%) and normal weight (0.0015+/-0.0003%; 0.082+/-0.008%) adults. There were no differences in EPC number between the overweight and normal weight groups. EPC colony formation was significantly less in the obese (6+/-1) and overweight (4+/-1) compared with normal weight (9+/-2) adults.

Conclusion: These results indicate that: (1) the number of circulating EPCs is lower in obese compared with overweight and normal weight adults; and (2) EPC colony-forming capacity is blunted in overweight and obese adults compared with normal weight adults. Impairments in EPC number and function may contribute to adiposity-related cardiovascular risk.

Show MeSH
Related in: MedlinePlus