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Van Gogh and Frizzled act redundantly in the Drosophila sensory organ precursor cell to orient its asymmetric division.

Gomes JE, Corado M, Schweisguth F - PLoS ONE (2009)

Bottom Line: Here, we investigate the relative contributions of Vang, Fz and Dishevelled (Dsh), a membrane-associated protein acting downstream of Fz, in orienting SOP polarity.Additionally, we find that the activity of Vang is dispensable for the non-autonomous polarizing activity of fz.These observations indicate that both Vang and Fz act as cues for downstream effectors orienting the planar polarity axis of dividing SOPs.

View Article: PubMed Central - PubMed

Affiliation: Ecole Normale Supérieure, CNRS UMR8542, Paris, France.

ABSTRACT
Drosophila sensory organ precursor cells (SOPs) divide asymmetrically along the anterior-posterior (a-p) body axis to generate two different daughter cells. Planar Cell Polarity (PCP) regulates the a-p orientation of the SOP division. The localization of the PCP proteins Van Gogh (Vang) and Frizzled (Fz) define anterior and posterior apical membrane domains prior to SOP division. Here, we investigate the relative contributions of Vang, Fz and Dishevelled (Dsh), a membrane-associated protein acting downstream of Fz, in orienting SOP polarity. Genetic and live imaging analyses suggest that Dsh restricts the localization of a centrosome-attracting activity to the anterior cortex and that Vang is a target of Dsh in this process. Using a clone border assay, we provide evidence that the Vang and fz genes act redundantly in SOPs to orient its polarity axis in response to extrinsic local PCP cues. Additionally, we find that the activity of Vang is dispensable for the non-autonomous polarizing activity of fz. These observations indicate that both Vang and Fz act as cues for downstream effectors orienting the planar polarity axis of dividing SOPs.

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Related in: MedlinePlus

Regulation of Pins cortical localization by Dsh is partly independent of Vang.The cortical localization of Pins (green) was examined in SOPs (Sens, red) in wild-type (A,A′), dsh1 (B,B′), Vangstbm6c (C,C′) and dsh1 Vangstbm6c double mutant (D,D′) SOPs. In D, nls-GFP (in blue) was used as clonal marker. The extent of the Pins-positive cortical domain in late prophase SOPs was measured as an angle value. The results of this quantification are shown in the bottom panels.
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pone-0004485-g002: Regulation of Pins cortical localization by Dsh is partly independent of Vang.The cortical localization of Pins (green) was examined in SOPs (Sens, red) in wild-type (A,A′), dsh1 (B,B′), Vangstbm6c (C,C′) and dsh1 Vangstbm6c double mutant (D,D′) SOPs. In D, nls-GFP (in blue) was used as clonal marker. The extent of the Pins-positive cortical domain in late prophase SOPs was measured as an angle value. The results of this quantification are shown in the bottom panels.

Mentions: Figure 2:


Van Gogh and Frizzled act redundantly in the Drosophila sensory organ precursor cell to orient its asymmetric division.

Gomes JE, Corado M, Schweisguth F - PLoS ONE (2009)

Regulation of Pins cortical localization by Dsh is partly independent of Vang.The cortical localization of Pins (green) was examined in SOPs (Sens, red) in wild-type (A,A′), dsh1 (B,B′), Vangstbm6c (C,C′) and dsh1 Vangstbm6c double mutant (D,D′) SOPs. In D, nls-GFP (in blue) was used as clonal marker. The extent of the Pins-positive cortical domain in late prophase SOPs was measured as an angle value. The results of this quantification are shown in the bottom panels.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2637423&req=5

pone-0004485-g002: Regulation of Pins cortical localization by Dsh is partly independent of Vang.The cortical localization of Pins (green) was examined in SOPs (Sens, red) in wild-type (A,A′), dsh1 (B,B′), Vangstbm6c (C,C′) and dsh1 Vangstbm6c double mutant (D,D′) SOPs. In D, nls-GFP (in blue) was used as clonal marker. The extent of the Pins-positive cortical domain in late prophase SOPs was measured as an angle value. The results of this quantification are shown in the bottom panels.
Mentions: Figure 2:

Bottom Line: Here, we investigate the relative contributions of Vang, Fz and Dishevelled (Dsh), a membrane-associated protein acting downstream of Fz, in orienting SOP polarity.Additionally, we find that the activity of Vang is dispensable for the non-autonomous polarizing activity of fz.These observations indicate that both Vang and Fz act as cues for downstream effectors orienting the planar polarity axis of dividing SOPs.

View Article: PubMed Central - PubMed

Affiliation: Ecole Normale Supérieure, CNRS UMR8542, Paris, France.

ABSTRACT
Drosophila sensory organ precursor cells (SOPs) divide asymmetrically along the anterior-posterior (a-p) body axis to generate two different daughter cells. Planar Cell Polarity (PCP) regulates the a-p orientation of the SOP division. The localization of the PCP proteins Van Gogh (Vang) and Frizzled (Fz) define anterior and posterior apical membrane domains prior to SOP division. Here, we investigate the relative contributions of Vang, Fz and Dishevelled (Dsh), a membrane-associated protein acting downstream of Fz, in orienting SOP polarity. Genetic and live imaging analyses suggest that Dsh restricts the localization of a centrosome-attracting activity to the anterior cortex and that Vang is a target of Dsh in this process. Using a clone border assay, we provide evidence that the Vang and fz genes act redundantly in SOPs to orient its polarity axis in response to extrinsic local PCP cues. Additionally, we find that the activity of Vang is dispensable for the non-autonomous polarizing activity of fz. These observations indicate that both Vang and Fz act as cues for downstream effectors orienting the planar polarity axis of dividing SOPs.

Show MeSH
Related in: MedlinePlus