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Differences in partitioning of meal fatty acids into blood lipid fractions: a comparison of linoleate, oleate, and palmitate.

Hodson L, McQuaid SE, Karpe F, Frayn KN, Fielding BA - Am. J. Physiol. Endocrinol. Metab. (2008)

Bottom Line: There has been much interest in the health effects of dietary fat, but few studies have comprehensively compared the acute metabolic fate of specific fatty acids in vivo.Using the values for isotopic enrichment in the different lipid fractions compared with the test meal, we calculated the contribution of meal fatty acids to the respective fractions.This was significantly greater than for oleate and palmitate (both 3 +/- 0.3%; P < 0.05).

View Article: PubMed Central - PubMed

Affiliation: Oxford Centre for Diabetes, Endocrinology, and Metabolism, Churchill Hospital, Oxford OX3 7LJ, UK. leanne.hodson@oxlip.ox.ac.uk

ABSTRACT
There has been much interest in the health effects of dietary fat, but few studies have comprehensively compared the acute metabolic fate of specific fatty acids in vivo. We hypothesized that different classes of fatty acids would be variably partitioned in metabolic pathways and that this would become evident over 24 h. We traced the fate of fatty acids using equal amounts of [U-(13)C]linoleate, [U-(13)C]oleate, and [U-(13)C]palmitate given in a test breakfast meal in 12 healthy subjects. There was a tendency for differences in the concentrations of the tracers in plasma chylomicron-triacylglycerol (TG) (oleate > palmitate > linoleate). This pattern remained in plasma nonesterified fatty acid (NEFA) and very low-density lipoprotein (VLDL)-TG (P

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Dietary [U-13C]linoleate (▾), [U-13C]oleate (○), and [U-13C]palmitate (•) incorporation into chylomicron-triacylglycerol (TG; A), plasma nonesterified fatty acid (NEFA; B), and very low-density lipoprotein (VLDL)-TG (C) after a mixed meal (0 h), a 75-g glucose drink (6 h), and a habitual diet. Values are means ± SE; n = 12.
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f1: Dietary [U-13C]linoleate (▾), [U-13C]oleate (○), and [U-13C]palmitate (•) incorporation into chylomicron-triacylglycerol (TG; A), plasma nonesterified fatty acid (NEFA; B), and very low-density lipoprotein (VLDL)-TG (C) after a mixed meal (0 h), a 75-g glucose drink (6 h), and a habitual diet. Values are means ± SE; n = 12.

Mentions: A similar pattern of incorporation of the [U-13C]fatty acids was observed in chylomicron-TG, with an increase in the amount of [U-13C]fatty acids occurring after the consumption of the second meal (at 6 h). There was a tendency for [U-13C]oleate to be higher than [U-13C]palmitate (P = 0.076) and [U-13C]linoleate (P = 0.066), and enrichment was not significantly different between [U-13C]palmitate and [U-13C]linoleate (Fig. 1A). There was a significant difference in the amount and pattern of [U-13C]fatty acids in plasma NEFA (P = 0.003); the concentration of [U-13C]oleate was significantly higher than [U-13C]palmitate and [U-13C]linoleate (P = 0.010 and P = 0.006, respectively; Fig. 1B). This pattern of difference between the fatty acids was maintained in VLDL-TG, with a significant difference in the amount of [U-13C]oleate compared with [U-13C]palmitate and [U-13C]linoleate (P = 0.02 and P = 0.018, respectively; Fig. 1C).


Differences in partitioning of meal fatty acids into blood lipid fractions: a comparison of linoleate, oleate, and palmitate.

Hodson L, McQuaid SE, Karpe F, Frayn KN, Fielding BA - Am. J. Physiol. Endocrinol. Metab. (2008)

Dietary [U-13C]linoleate (▾), [U-13C]oleate (○), and [U-13C]palmitate (•) incorporation into chylomicron-triacylglycerol (TG; A), plasma nonesterified fatty acid (NEFA; B), and very low-density lipoprotein (VLDL)-TG (C) after a mixed meal (0 h), a 75-g glucose drink (6 h), and a habitual diet. Values are means ± SE; n = 12.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2636984&req=5

f1: Dietary [U-13C]linoleate (▾), [U-13C]oleate (○), and [U-13C]palmitate (•) incorporation into chylomicron-triacylglycerol (TG; A), plasma nonesterified fatty acid (NEFA; B), and very low-density lipoprotein (VLDL)-TG (C) after a mixed meal (0 h), a 75-g glucose drink (6 h), and a habitual diet. Values are means ± SE; n = 12.
Mentions: A similar pattern of incorporation of the [U-13C]fatty acids was observed in chylomicron-TG, with an increase in the amount of [U-13C]fatty acids occurring after the consumption of the second meal (at 6 h). There was a tendency for [U-13C]oleate to be higher than [U-13C]palmitate (P = 0.076) and [U-13C]linoleate (P = 0.066), and enrichment was not significantly different between [U-13C]palmitate and [U-13C]linoleate (Fig. 1A). There was a significant difference in the amount and pattern of [U-13C]fatty acids in plasma NEFA (P = 0.003); the concentration of [U-13C]oleate was significantly higher than [U-13C]palmitate and [U-13C]linoleate (P = 0.010 and P = 0.006, respectively; Fig. 1B). This pattern of difference between the fatty acids was maintained in VLDL-TG, with a significant difference in the amount of [U-13C]oleate compared with [U-13C]palmitate and [U-13C]linoleate (P = 0.02 and P = 0.018, respectively; Fig. 1C).

Bottom Line: There has been much interest in the health effects of dietary fat, but few studies have comprehensively compared the acute metabolic fate of specific fatty acids in vivo.Using the values for isotopic enrichment in the different lipid fractions compared with the test meal, we calculated the contribution of meal fatty acids to the respective fractions.This was significantly greater than for oleate and palmitate (both 3 +/- 0.3%; P < 0.05).

View Article: PubMed Central - PubMed

Affiliation: Oxford Centre for Diabetes, Endocrinology, and Metabolism, Churchill Hospital, Oxford OX3 7LJ, UK. leanne.hodson@oxlip.ox.ac.uk

ABSTRACT
There has been much interest in the health effects of dietary fat, but few studies have comprehensively compared the acute metabolic fate of specific fatty acids in vivo. We hypothesized that different classes of fatty acids would be variably partitioned in metabolic pathways and that this would become evident over 24 h. We traced the fate of fatty acids using equal amounts of [U-(13)C]linoleate, [U-(13)C]oleate, and [U-(13)C]palmitate given in a test breakfast meal in 12 healthy subjects. There was a tendency for differences in the concentrations of the tracers in plasma chylomicron-triacylglycerol (TG) (oleate > palmitate > linoleate). This pattern remained in plasma nonesterified fatty acid (NEFA) and very low-density lipoprotein (VLDL)-TG (P

Show MeSH