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Detection of hydrogen peroxide with chemiluminescent micelles.

Lee D, Erigala VR, Dasari M, Yu J, Dickson RM, Murthy N - Int J Nanomedicine (2008)

Bottom Line: In this communication, we present a new contrast agent for hydrogen peroxide, termed peroxalate micelles, which detect hydrogen peroxide through chemiluminescence, and have the physical/chemical properties needed for in vivo imaging applications.The peroxalate micelles can detect nanomolar concentrations of hydrogen peroxide (>50 nM) and thus have the sensitivity needed to detect physiological concentrations of hydrogen peroxide.We anticipate numerous applications of the peroxalate micelles for in vivo imaging of hydrogen peroxide, given their high sensitivity, small size, and biocompatible PEG corona.

View Article: PubMed Central - PubMed

Affiliation: The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA.

ABSTRACT
The overproduction of hydrogen peroxide is implicated in the progress of numerous life-threatening diseases and there is a great need for the development of contrast agents that can detect hydrogen peroxide in vivo. In this communication, we present a new contrast agent for hydrogen peroxide, termed peroxalate micelles, which detect hydrogen peroxide through chemiluminescence, and have the physical/chemical properties needed for in vivo imaging applications. The peroxalate micelles are composed of amphiphilic peroxalate based copolymers and the fluorescent dye rubrene, they have a 'stealth' polyethylene glycol (PEG) corona to evade macrophage phagocytosis, and a diameter of 33 nm to enhance extravasation into permeable tissues. The peroxalate micelles can detect nanomolar concentrations of hydrogen peroxide (>50 nM) and thus have the sensitivity needed to detect physiological concentrations of hydrogen peroxide. We anticipate numerous applications of the peroxalate micelles for in vivo imaging of hydrogen peroxide, given their high sensitivity, small size, and biocompatible PEG corona.

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Sensitivity of peroxalate micelles to hydrogen peroxide. Data were obtained from 1 mL of micelles (1 mg/mL) in response to increasing concentrations of hydrogen peroxide. RLU, relative light intensity. Mean ± SD, n = 3. The inset shows the chemiluminescence response to hydrogen peroxide in the range of 0 to 100 nM.
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f3-ijn-3-471: Sensitivity of peroxalate micelles to hydrogen peroxide. Data were obtained from 1 mL of micelles (1 mg/mL) in response to increasing concentrations of hydrogen peroxide. RLU, relative light intensity. Mean ± SD, n = 3. The inset shows the chemiluminescence response to hydrogen peroxide in the range of 0 to 100 nM.

Mentions: The ability of the peroxalate micelles to detect hydrogen peroxide was investigated by measuring their chemiluminescence intensity in the presence of hydrogen peroxide at various concentrations in the range of 0–1 μM. The intracellular concentration of hydrogen peroxide appears to be in the range of 1 nM to 700 nM (Stone 2004; Miller et al 2005). The Figure 3 demonstrates that the peroxalate micelles have excellent sensitivity to hydrogen peroxide and exhibit a linear correlation between chemiluminescence intensity and hydrogen peroxide concentration. The peroxalate micelles were capable of detecting hydrogen peroxide at concentrations as low as 50 nM (18603 RLU/s at 50 nM vs 252 RLU/s at 0 nM), suggesting great potential for the detection of hydrogen peroxide at physiological concentrations. The half-life of peroxalate chemiluminescence was approximately 1.5 min in the presence of hydrogen peroxide. Interestingly, the peroxalate micelles have a sensitivity for hydrogen peroxide that is 5-fold greater than the larger peroxalate nanoparticles (50 nM vs 250 nM) (Lee et al 2007). We speculate that the enhanced sensitivity of the peroxalate micelles is partially due to their smaller size and higher surface area, which should enhance the diffusion of hydrogen peroxide into the micelles.


Detection of hydrogen peroxide with chemiluminescent micelles.

Lee D, Erigala VR, Dasari M, Yu J, Dickson RM, Murthy N - Int J Nanomedicine (2008)

Sensitivity of peroxalate micelles to hydrogen peroxide. Data were obtained from 1 mL of micelles (1 mg/mL) in response to increasing concentrations of hydrogen peroxide. RLU, relative light intensity. Mean ± SD, n = 3. The inset shows the chemiluminescence response to hydrogen peroxide in the range of 0 to 100 nM.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC2636589&req=5

f3-ijn-3-471: Sensitivity of peroxalate micelles to hydrogen peroxide. Data were obtained from 1 mL of micelles (1 mg/mL) in response to increasing concentrations of hydrogen peroxide. RLU, relative light intensity. Mean ± SD, n = 3. The inset shows the chemiluminescence response to hydrogen peroxide in the range of 0 to 100 nM.
Mentions: The ability of the peroxalate micelles to detect hydrogen peroxide was investigated by measuring their chemiluminescence intensity in the presence of hydrogen peroxide at various concentrations in the range of 0–1 μM. The intracellular concentration of hydrogen peroxide appears to be in the range of 1 nM to 700 nM (Stone 2004; Miller et al 2005). The Figure 3 demonstrates that the peroxalate micelles have excellent sensitivity to hydrogen peroxide and exhibit a linear correlation between chemiluminescence intensity and hydrogen peroxide concentration. The peroxalate micelles were capable of detecting hydrogen peroxide at concentrations as low as 50 nM (18603 RLU/s at 50 nM vs 252 RLU/s at 0 nM), suggesting great potential for the detection of hydrogen peroxide at physiological concentrations. The half-life of peroxalate chemiluminescence was approximately 1.5 min in the presence of hydrogen peroxide. Interestingly, the peroxalate micelles have a sensitivity for hydrogen peroxide that is 5-fold greater than the larger peroxalate nanoparticles (50 nM vs 250 nM) (Lee et al 2007). We speculate that the enhanced sensitivity of the peroxalate micelles is partially due to their smaller size and higher surface area, which should enhance the diffusion of hydrogen peroxide into the micelles.

Bottom Line: In this communication, we present a new contrast agent for hydrogen peroxide, termed peroxalate micelles, which detect hydrogen peroxide through chemiluminescence, and have the physical/chemical properties needed for in vivo imaging applications.The peroxalate micelles can detect nanomolar concentrations of hydrogen peroxide (>50 nM) and thus have the sensitivity needed to detect physiological concentrations of hydrogen peroxide.We anticipate numerous applications of the peroxalate micelles for in vivo imaging of hydrogen peroxide, given their high sensitivity, small size, and biocompatible PEG corona.

View Article: PubMed Central - PubMed

Affiliation: The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA.

ABSTRACT
The overproduction of hydrogen peroxide is implicated in the progress of numerous life-threatening diseases and there is a great need for the development of contrast agents that can detect hydrogen peroxide in vivo. In this communication, we present a new contrast agent for hydrogen peroxide, termed peroxalate micelles, which detect hydrogen peroxide through chemiluminescence, and have the physical/chemical properties needed for in vivo imaging applications. The peroxalate micelles are composed of amphiphilic peroxalate based copolymers and the fluorescent dye rubrene, they have a 'stealth' polyethylene glycol (PEG) corona to evade macrophage phagocytosis, and a diameter of 33 nm to enhance extravasation into permeable tissues. The peroxalate micelles can detect nanomolar concentrations of hydrogen peroxide (>50 nM) and thus have the sensitivity needed to detect physiological concentrations of hydrogen peroxide. We anticipate numerous applications of the peroxalate micelles for in vivo imaging of hydrogen peroxide, given their high sensitivity, small size, and biocompatible PEG corona.

Show MeSH
Related in: MedlinePlus