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Influence of nanophase titania topography on bacterial attachment and metabolism.

Park MR, Banks MK, Applegate B, Webster TJ - Int J Nanomedicine (2008)

Bottom Line: Surfaces with nanophase compared to conventional (or nanometer smooth) topographies are known to have different properties of area, charge, and reactivity.Previously published research indicates that the attachment of certain bacteria (such as Pseudomonas fluorescens 5RL) is higher on surfaces with nanophase compared to conventional topographies, however, their effect on bacterial metabolism is unclear.Thus, the results from this study show greater select bacterial metabolism on nanometer than conventional topographies, critical results with strong consequences for the design of improved biosensors for bacteria detection.

View Article: PubMed Central - PubMed

Affiliation: School of Civil Engineering, Purdue University, West Lafayette, IN, USA.

ABSTRACT
Surfaces with nanophase compared to conventional (or nanometer smooth) topographies are known to have different properties of area, charge, and reactivity. Previously published research indicates that the attachment of certain bacteria (such as Pseudomonas fluorescens 5RL) is higher on surfaces with nanophase compared to conventional topographies, however, their effect on bacterial metabolism is unclear. Results presented here show that the adhesion of Pseudomonas fluorescens 5RL and Pseudomonas putida TVA8 was higher on nanophase than conventional titania. Importantly, in terms of metabolism, bacteria attached to the nanophase surfaces had higher bioluminescence rates than on the conventional surfaces under all nutrient conditions. Thus, the results from this study show greater select bacterial metabolism on nanometer than conventional topographies, critical results with strong consequences for the design of improved biosensors for bacteria detection.

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Related in: MedlinePlus

Light emission (a) and number (b) of attached bioluminescent bacteria Pseudomonas fluorescens 5RL on titania as affected by the presence of a carbon source in the solution phase. Background luminesence was 960 photons/sec. All data are significantly different (p < 0.01) comparing respective conditions of nanophase to conventional titania.
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f3-ijn-3-497: Light emission (a) and number (b) of attached bioluminescent bacteria Pseudomonas fluorescens 5RL on titania as affected by the presence of a carbon source in the solution phase. Background luminesence was 960 photons/sec. All data are significantly different (p < 0.01) comparing respective conditions of nanophase to conventional titania.

Mentions: The attachment of P. fluorescens 5RL and P. putida TVA8 was assessed on surfaces with differing topographies and nutrient conditions. P. fluorescens had a higher level of attachment and light emission on the nanophase material when compared to the conventional surface (Figure 3). No spatial differences in bacteria attachment were observed on the substrates. The presence of glucose in the post-attachment solution resulted in a small increase of microbial numbers on the surface and a higher emission of light for the conventional surface. On the nanophase surface, the solution containing glucose also enhanced bacterial numbers and light emission, although to a larger degree than on the conventional surface.


Influence of nanophase titania topography on bacterial attachment and metabolism.

Park MR, Banks MK, Applegate B, Webster TJ - Int J Nanomedicine (2008)

Light emission (a) and number (b) of attached bioluminescent bacteria Pseudomonas fluorescens 5RL on titania as affected by the presence of a carbon source in the solution phase. Background luminesence was 960 photons/sec. All data are significantly different (p < 0.01) comparing respective conditions of nanophase to conventional titania.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2636588&req=5

f3-ijn-3-497: Light emission (a) and number (b) of attached bioluminescent bacteria Pseudomonas fluorescens 5RL on titania as affected by the presence of a carbon source in the solution phase. Background luminesence was 960 photons/sec. All data are significantly different (p < 0.01) comparing respective conditions of nanophase to conventional titania.
Mentions: The attachment of P. fluorescens 5RL and P. putida TVA8 was assessed on surfaces with differing topographies and nutrient conditions. P. fluorescens had a higher level of attachment and light emission on the nanophase material when compared to the conventional surface (Figure 3). No spatial differences in bacteria attachment were observed on the substrates. The presence of glucose in the post-attachment solution resulted in a small increase of microbial numbers on the surface and a higher emission of light for the conventional surface. On the nanophase surface, the solution containing glucose also enhanced bacterial numbers and light emission, although to a larger degree than on the conventional surface.

Bottom Line: Surfaces with nanophase compared to conventional (or nanometer smooth) topographies are known to have different properties of area, charge, and reactivity.Previously published research indicates that the attachment of certain bacteria (such as Pseudomonas fluorescens 5RL) is higher on surfaces with nanophase compared to conventional topographies, however, their effect on bacterial metabolism is unclear.Thus, the results from this study show greater select bacterial metabolism on nanometer than conventional topographies, critical results with strong consequences for the design of improved biosensors for bacteria detection.

View Article: PubMed Central - PubMed

Affiliation: School of Civil Engineering, Purdue University, West Lafayette, IN, USA.

ABSTRACT
Surfaces with nanophase compared to conventional (or nanometer smooth) topographies are known to have different properties of area, charge, and reactivity. Previously published research indicates that the attachment of certain bacteria (such as Pseudomonas fluorescens 5RL) is higher on surfaces with nanophase compared to conventional topographies, however, their effect on bacterial metabolism is unclear. Results presented here show that the adhesion of Pseudomonas fluorescens 5RL and Pseudomonas putida TVA8 was higher on nanophase than conventional titania. Importantly, in terms of metabolism, bacteria attached to the nanophase surfaces had higher bioluminescence rates than on the conventional surfaces under all nutrient conditions. Thus, the results from this study show greater select bacterial metabolism on nanometer than conventional topographies, critical results with strong consequences for the design of improved biosensors for bacteria detection.

Show MeSH
Related in: MedlinePlus