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Influence of nanophase titania topography on bacterial attachment and metabolism.

Park MR, Banks MK, Applegate B, Webster TJ - Int J Nanomedicine (2008)

Bottom Line: Surfaces with nanophase compared to conventional (or nanometer smooth) topographies are known to have different properties of area, charge, and reactivity.Previously published research indicates that the attachment of certain bacteria (such as Pseudomonas fluorescens 5RL) is higher on surfaces with nanophase compared to conventional topographies, however, their effect on bacterial metabolism is unclear.Thus, the results from this study show greater select bacterial metabolism on nanometer than conventional topographies, critical results with strong consequences for the design of improved biosensors for bacteria detection.

View Article: PubMed Central - PubMed

Affiliation: School of Civil Engineering, Purdue University, West Lafayette, IN, USA.

ABSTRACT
Surfaces with nanophase compared to conventional (or nanometer smooth) topographies are known to have different properties of area, charge, and reactivity. Previously published research indicates that the attachment of certain bacteria (such as Pseudomonas fluorescens 5RL) is higher on surfaces with nanophase compared to conventional topographies, however, their effect on bacterial metabolism is unclear. Results presented here show that the adhesion of Pseudomonas fluorescens 5RL and Pseudomonas putida TVA8 was higher on nanophase than conventional titania. Importantly, in terms of metabolism, bacteria attached to the nanophase surfaces had higher bioluminescence rates than on the conventional surfaces under all nutrient conditions. Thus, the results from this study show greater select bacterial metabolism on nanometer than conventional topographies, critical results with strong consequences for the design of improved biosensors for bacteria detection.

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Related in: MedlinePlus

XRD spectra of nanophase and conventional titania illustrating a mixture of anatase and rutile crystal phases. Similar crystal phases were observed for both nanophase and conventional titania, thus, as an example, nanophase titania is only shown here.Abbreviation: XRD, X-ray diffraction.
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f2-ijn-3-497: XRD spectra of nanophase and conventional titania illustrating a mixture of anatase and rutile crystal phases. Similar crystal phases were observed for both nanophase and conventional titania, thus, as an example, nanophase titania is only shown here.Abbreviation: XRD, X-ray diffraction.

Mentions: Material characterization studies provided evidence that nanophase titania was considerably more rough at the nanoscale than conventional titania (specifically, AFM root-mean-square roughness values were 32 nm and 16 nm for nanophase and conventional titania, respectively) (Figure 1). BET measurements also provided evidence that the average grain size for the nanoscale and conventional titania were 32 nm and 2.12 μm, respectively. X-ray diffraction spectra showed that the material phases did not change when titania was sintered at 600° compared to 1200 °C (40% rutile and 60% anatase) (Figure 2).


Influence of nanophase titania topography on bacterial attachment and metabolism.

Park MR, Banks MK, Applegate B, Webster TJ - Int J Nanomedicine (2008)

XRD spectra of nanophase and conventional titania illustrating a mixture of anatase and rutile crystal phases. Similar crystal phases were observed for both nanophase and conventional titania, thus, as an example, nanophase titania is only shown here.Abbreviation: XRD, X-ray diffraction.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2636588&req=5

f2-ijn-3-497: XRD spectra of nanophase and conventional titania illustrating a mixture of anatase and rutile crystal phases. Similar crystal phases were observed for both nanophase and conventional titania, thus, as an example, nanophase titania is only shown here.Abbreviation: XRD, X-ray diffraction.
Mentions: Material characterization studies provided evidence that nanophase titania was considerably more rough at the nanoscale than conventional titania (specifically, AFM root-mean-square roughness values were 32 nm and 16 nm for nanophase and conventional titania, respectively) (Figure 1). BET measurements also provided evidence that the average grain size for the nanoscale and conventional titania were 32 nm and 2.12 μm, respectively. X-ray diffraction spectra showed that the material phases did not change when titania was sintered at 600° compared to 1200 °C (40% rutile and 60% anatase) (Figure 2).

Bottom Line: Surfaces with nanophase compared to conventional (or nanometer smooth) topographies are known to have different properties of area, charge, and reactivity.Previously published research indicates that the attachment of certain bacteria (such as Pseudomonas fluorescens 5RL) is higher on surfaces with nanophase compared to conventional topographies, however, their effect on bacterial metabolism is unclear.Thus, the results from this study show greater select bacterial metabolism on nanometer than conventional topographies, critical results with strong consequences for the design of improved biosensors for bacteria detection.

View Article: PubMed Central - PubMed

Affiliation: School of Civil Engineering, Purdue University, West Lafayette, IN, USA.

ABSTRACT
Surfaces with nanophase compared to conventional (or nanometer smooth) topographies are known to have different properties of area, charge, and reactivity. Previously published research indicates that the attachment of certain bacteria (such as Pseudomonas fluorescens 5RL) is higher on surfaces with nanophase compared to conventional topographies, however, their effect on bacterial metabolism is unclear. Results presented here show that the adhesion of Pseudomonas fluorescens 5RL and Pseudomonas putida TVA8 was higher on nanophase than conventional titania. Importantly, in terms of metabolism, bacteria attached to the nanophase surfaces had higher bioluminescence rates than on the conventional surfaces under all nutrient conditions. Thus, the results from this study show greater select bacterial metabolism on nanometer than conventional topographies, critical results with strong consequences for the design of improved biosensors for bacteria detection.

Show MeSH
Related in: MedlinePlus