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Impact of surface electric properties of carbon-based thin films on platelets activation for nano-medical and nano-sensing applications.

Karagkiozaki V, Logothetidis S, Lousinian S, Giannoglou G - Int J Nanomedicine (2008)

Bottom Line: Platelet-rich plasma drawn from healthy donors was used and semi-contact mode of AFM was applied.Platelets behavior and their correlation with the electric surface properties of the examined a-C:H films by EFM was made for hemocompatibility enhancement and sensing platelets that are less electrical negatively charged and with higher tendency to aggregate and form thrombus.The results are discussed in view of the effect of different deposition conditions of hydrogenated carbon films on their structural and morphological characteristics, surface roughness and electrical properties attributing to different hemocompatibility and sensing aspects.

View Article: PubMed Central - PubMed

Affiliation: Physics Department, Laboratory for Thin Films-Nanosystems and Nanometrology (LTFN), Medical School, AHEPA University General Hospital, 1st Cardiology Department, Aristotle University of Thessaloniki, Greece.

ABSTRACT
Electric surface properties of biomaterials, playing key role to various biointerfacial interactions, were related to hemocompatibility and biosensing phenomena. In this study, the examination of surface electric properties of amorphous hydrogenated carbon thin films (a-C:H) was carried out by means of electrostatic force microscope (EFM) and observation of differences in spatial charge distribution on the surface of the examined films during platelets adhesion was made. The thrombogenic potential of a-C:H thin films developed by magnetron sputtering with approximately 42% sp(3) content and hydrogen partial pressure during deposition was evaluated, by in situ observation with atomic force microscope (AFM) of platelets' activation and their subsequent adhesion. Platelet-rich plasma drawn from healthy donors was used and semi-contact mode of AFM was applied. Platelets behavior and their correlation with the electric surface properties of the examined a-C:H films by EFM was made for hemocompatibility enhancement and sensing platelets that are less electrical negatively charged and with higher tendency to aggregate and form thrombus. The results are discussed in view of the effect of different deposition conditions of hydrogenated carbon films on their structural and morphological characteristics, surface roughness and electrical properties attributing to different hemocompatibility and sensing aspects.

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Atomic force microscope topography image of (a) platelets on a-C:H (Biased) after 1 hour incubation time (scan size 10 μm × 10 μm) (b) Platelets on a-C:H (Biased) after 2 hours incubation time, with a size of 20 μm × 20 μm.
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f5-ijn-3-461: Atomic force microscope topography image of (a) platelets on a-C:H (Biased) after 1 hour incubation time (scan size 10 μm × 10 μm) (b) Platelets on a-C:H (Biased) after 2 hours incubation time, with a size of 20 μm × 20 μm.

Mentions: In contrast, on the biased carbon films, platelets have a higher tendency to aggregate and this can be easily noticed by comparing the AFM images after 1 and 2 hours incubation time with the images of floating platelets. This fact can be verified by the comparative diagram of Rrms and peak-to-peak parameters versus platelets incubation time (as shown in Figures 3 and 4). It is also observed in AFM topography images (Figure 5), that biased a-C:H thin films like floating platelets, after one hour of platelets incubation, form clusters increasing in height and after two hours of study, they present the ‘island’ type formation. Thus, this model of platelets aggregation can be suggested for the evaluation of thrombogenicity of biomaterials.


Impact of surface electric properties of carbon-based thin films on platelets activation for nano-medical and nano-sensing applications.

Karagkiozaki V, Logothetidis S, Lousinian S, Giannoglou G - Int J Nanomedicine (2008)

Atomic force microscope topography image of (a) platelets on a-C:H (Biased) after 1 hour incubation time (scan size 10 μm × 10 μm) (b) Platelets on a-C:H (Biased) after 2 hours incubation time, with a size of 20 μm × 20 μm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2636585&req=5

f5-ijn-3-461: Atomic force microscope topography image of (a) platelets on a-C:H (Biased) after 1 hour incubation time (scan size 10 μm × 10 μm) (b) Platelets on a-C:H (Biased) after 2 hours incubation time, with a size of 20 μm × 20 μm.
Mentions: In contrast, on the biased carbon films, platelets have a higher tendency to aggregate and this can be easily noticed by comparing the AFM images after 1 and 2 hours incubation time with the images of floating platelets. This fact can be verified by the comparative diagram of Rrms and peak-to-peak parameters versus platelets incubation time (as shown in Figures 3 and 4). It is also observed in AFM topography images (Figure 5), that biased a-C:H thin films like floating platelets, after one hour of platelets incubation, form clusters increasing in height and after two hours of study, they present the ‘island’ type formation. Thus, this model of platelets aggregation can be suggested for the evaluation of thrombogenicity of biomaterials.

Bottom Line: Platelet-rich plasma drawn from healthy donors was used and semi-contact mode of AFM was applied.Platelets behavior and their correlation with the electric surface properties of the examined a-C:H films by EFM was made for hemocompatibility enhancement and sensing platelets that are less electrical negatively charged and with higher tendency to aggregate and form thrombus.The results are discussed in view of the effect of different deposition conditions of hydrogenated carbon films on their structural and morphological characteristics, surface roughness and electrical properties attributing to different hemocompatibility and sensing aspects.

View Article: PubMed Central - PubMed

Affiliation: Physics Department, Laboratory for Thin Films-Nanosystems and Nanometrology (LTFN), Medical School, AHEPA University General Hospital, 1st Cardiology Department, Aristotle University of Thessaloniki, Greece.

ABSTRACT
Electric surface properties of biomaterials, playing key role to various biointerfacial interactions, were related to hemocompatibility and biosensing phenomena. In this study, the examination of surface electric properties of amorphous hydrogenated carbon thin films (a-C:H) was carried out by means of electrostatic force microscope (EFM) and observation of differences in spatial charge distribution on the surface of the examined films during platelets adhesion was made. The thrombogenic potential of a-C:H thin films developed by magnetron sputtering with approximately 42% sp(3) content and hydrogen partial pressure during deposition was evaluated, by in situ observation with atomic force microscope (AFM) of platelets' activation and their subsequent adhesion. Platelet-rich plasma drawn from healthy donors was used and semi-contact mode of AFM was applied. Platelets behavior and their correlation with the electric surface properties of the examined a-C:H films by EFM was made for hemocompatibility enhancement and sensing platelets that are less electrical negatively charged and with higher tendency to aggregate and form thrombus. The results are discussed in view of the effect of different deposition conditions of hydrogenated carbon films on their structural and morphological characteristics, surface roughness and electrical properties attributing to different hemocompatibility and sensing aspects.

Show MeSH
Related in: MedlinePlus