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Preparation, characterization and in-vitro evaluation of sustained release protein-loaded nanoparticles based on biodegradable polymers.

Mukherjee B, Santra K, Pattnaik G, Ghosh S - Int J Nanomedicine (2008)

Bottom Line: Controlled drug delivery technology of proteins/peptides from biodegradable nanoparticles has emerged as one of the eminent areas to overcome formulation associated problems of the macromolecules.The purpose of the present investigation was to develop protein-loaded nanoparticles using biodegradable polymer poly L-lactide-co-glycolidic acid (PLGA) with bovine serum albumin (BSA) as a model protein.The formulation prepared with protein-polymer ratio of 1:60 at 17,500 rpm gave maximum protein-loading, minimum polydispersion with maximally sustained protein release pattern, among the prepared formulations.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Technology, Jadavpur University, Kolkata, West Bengal, India. biswajit55@yahoo.com

ABSTRACT
Controlled drug delivery technology of proteins/peptides from biodegradable nanoparticles has emerged as one of the eminent areas to overcome formulation associated problems of the macromolecules. The purpose of the present investigation was to develop protein-loaded nanoparticles using biodegradable polymer poly L-lactide-co-glycolidic acid (PLGA) with bovine serum albumin (BSA) as a model protein. Despite many studies available with PLGA-based protein-loaded nanoparticles, production know-how, process parameters, protein loading, duration of protein release, narrowing polydispersity of particles have not been investigated enough to scale up manufacturing of protein-loaded nanoparticles in formulations. Different process parameters such as protein/polymer ratio, homogenizing speed during emulsifications, particle surface morphology and surface charges, particle size analysis and in-vitro protein release were investigated. The in-vitro protein release study suggests that release profile of BSA from nanoparticles could be modulated by changing protein-polymer ratios and/or by varying homogenizing speed during multiple-emulsion preparation technique. The formulation prepared with protein-polymer ratio of 1:60 at 17,500 rpm gave maximum protein-loading, minimum polydispersion with maximally sustained protein release pattern, among the prepared formulations. Decreased (10,000 rpm) or enhanced (24,000 rpm) homogenizing speeds resulted in increased polydispersion with larger particles having no better protein-loading and -release profiles in the present study.

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Cumulative percentage of BSA released from the experimental formulations. Data shows mean ± SD (n = 6).
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f7-ijn-3-487: Cumulative percentage of BSA released from the experimental formulations. Data shows mean ± SD (n = 6).

Mentions: When protein release study was conducted it was found that the cumulative amount of the protein release in 50 days was 83.52 μg per mg of formulation in case of G1. The values were 82.31, 72.35, 60.75 μg per mg of formulation in case of G2, G3 and G4, respectively (Figure 6). When the cumulative percentages of the protein release from the experimental formulations were plotted against time, it was found that about 84% protein was released in 50 days in case of the formulation G3, whereas, G4 had a release of about 42% in the same time period (Figure 7). G1 and G2 had a cumulative percentage release of about 61% and 77%, respectively.


Preparation, characterization and in-vitro evaluation of sustained release protein-loaded nanoparticles based on biodegradable polymers.

Mukherjee B, Santra K, Pattnaik G, Ghosh S - Int J Nanomedicine (2008)

Cumulative percentage of BSA released from the experimental formulations. Data shows mean ± SD (n = 6).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2636584&req=5

f7-ijn-3-487: Cumulative percentage of BSA released from the experimental formulations. Data shows mean ± SD (n = 6).
Mentions: When protein release study was conducted it was found that the cumulative amount of the protein release in 50 days was 83.52 μg per mg of formulation in case of G1. The values were 82.31, 72.35, 60.75 μg per mg of formulation in case of G2, G3 and G4, respectively (Figure 6). When the cumulative percentages of the protein release from the experimental formulations were plotted against time, it was found that about 84% protein was released in 50 days in case of the formulation G3, whereas, G4 had a release of about 42% in the same time period (Figure 7). G1 and G2 had a cumulative percentage release of about 61% and 77%, respectively.

Bottom Line: Controlled drug delivery technology of proteins/peptides from biodegradable nanoparticles has emerged as one of the eminent areas to overcome formulation associated problems of the macromolecules.The purpose of the present investigation was to develop protein-loaded nanoparticles using biodegradable polymer poly L-lactide-co-glycolidic acid (PLGA) with bovine serum albumin (BSA) as a model protein.The formulation prepared with protein-polymer ratio of 1:60 at 17,500 rpm gave maximum protein-loading, minimum polydispersion with maximally sustained protein release pattern, among the prepared formulations.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Technology, Jadavpur University, Kolkata, West Bengal, India. biswajit55@yahoo.com

ABSTRACT
Controlled drug delivery technology of proteins/peptides from biodegradable nanoparticles has emerged as one of the eminent areas to overcome formulation associated problems of the macromolecules. The purpose of the present investigation was to develop protein-loaded nanoparticles using biodegradable polymer poly L-lactide-co-glycolidic acid (PLGA) with bovine serum albumin (BSA) as a model protein. Despite many studies available with PLGA-based protein-loaded nanoparticles, production know-how, process parameters, protein loading, duration of protein release, narrowing polydispersity of particles have not been investigated enough to scale up manufacturing of protein-loaded nanoparticles in formulations. Different process parameters such as protein/polymer ratio, homogenizing speed during emulsifications, particle surface morphology and surface charges, particle size analysis and in-vitro protein release were investigated. The in-vitro protein release study suggests that release profile of BSA from nanoparticles could be modulated by changing protein-polymer ratios and/or by varying homogenizing speed during multiple-emulsion preparation technique. The formulation prepared with protein-polymer ratio of 1:60 at 17,500 rpm gave maximum protein-loading, minimum polydispersion with maximally sustained protein release pattern, among the prepared formulations. Decreased (10,000 rpm) or enhanced (24,000 rpm) homogenizing speeds resulted in increased polydispersion with larger particles having no better protein-loading and -release profiles in the present study.

Show MeSH
Related in: MedlinePlus