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Preparation, characterization and in-vitro evaluation of sustained release protein-loaded nanoparticles based on biodegradable polymers.

Mukherjee B, Santra K, Pattnaik G, Ghosh S - Int J Nanomedicine (2008)

Bottom Line: Controlled drug delivery technology of proteins/peptides from biodegradable nanoparticles has emerged as one of the eminent areas to overcome formulation associated problems of the macromolecules.The purpose of the present investigation was to develop protein-loaded nanoparticles using biodegradable polymer poly L-lactide-co-glycolidic acid (PLGA) with bovine serum albumin (BSA) as a model protein.The formulation prepared with protein-polymer ratio of 1:60 at 17,500 rpm gave maximum protein-loading, minimum polydispersion with maximally sustained protein release pattern, among the prepared formulations.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Technology, Jadavpur University, Kolkata, West Bengal, India. biswajit55@yahoo.com

ABSTRACT
Controlled drug delivery technology of proteins/peptides from biodegradable nanoparticles has emerged as one of the eminent areas to overcome formulation associated problems of the macromolecules. The purpose of the present investigation was to develop protein-loaded nanoparticles using biodegradable polymer poly L-lactide-co-glycolidic acid (PLGA) with bovine serum albumin (BSA) as a model protein. Despite many studies available with PLGA-based protein-loaded nanoparticles, production know-how, process parameters, protein loading, duration of protein release, narrowing polydispersity of particles have not been investigated enough to scale up manufacturing of protein-loaded nanoparticles in formulations. Different process parameters such as protein/polymer ratio, homogenizing speed during emulsifications, particle surface morphology and surface charges, particle size analysis and in-vitro protein release were investigated. The in-vitro protein release study suggests that release profile of BSA from nanoparticles could be modulated by changing protein-polymer ratios and/or by varying homogenizing speed during multiple-emulsion preparation technique. The formulation prepared with protein-polymer ratio of 1:60 at 17,500 rpm gave maximum protein-loading, minimum polydispersion with maximally sustained protein release pattern, among the prepared formulations. Decreased (10,000 rpm) or enhanced (24,000 rpm) homogenizing speeds resulted in increased polydispersion with larger particles having no better protein-loading and -release profiles in the present study.

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FTIR spectra of excipients.
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f2-ijn-3-487: FTIR spectra of excipients.

Mentions: Drug-excipient interaction study is an important study to formulate a chemical entity with the other chemicals in a sustained release delivery system, so that the desire release pattern and other requisite physico-chemical characteristics may be achieved (Mukherjee et al 2005a). Among the various methods available, FTIR spectroscopy provides us a distinct idea regarding interaction between various functional groups present in drug and excipients (Cunha-Filho et al 2007). In the present study when the FTIR spectrum of BSA, PLGA-PVA and BSA with PLGA-PVA (Figures 1, 2, and 3) were compared, no physico-chemical interaction was detected. Hence, the polymer was used to formulate the protein.


Preparation, characterization and in-vitro evaluation of sustained release protein-loaded nanoparticles based on biodegradable polymers.

Mukherjee B, Santra K, Pattnaik G, Ghosh S - Int J Nanomedicine (2008)

FTIR spectra of excipients.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2636584&req=5

f2-ijn-3-487: FTIR spectra of excipients.
Mentions: Drug-excipient interaction study is an important study to formulate a chemical entity with the other chemicals in a sustained release delivery system, so that the desire release pattern and other requisite physico-chemical characteristics may be achieved (Mukherjee et al 2005a). Among the various methods available, FTIR spectroscopy provides us a distinct idea regarding interaction between various functional groups present in drug and excipients (Cunha-Filho et al 2007). In the present study when the FTIR spectrum of BSA, PLGA-PVA and BSA with PLGA-PVA (Figures 1, 2, and 3) were compared, no physico-chemical interaction was detected. Hence, the polymer was used to formulate the protein.

Bottom Line: Controlled drug delivery technology of proteins/peptides from biodegradable nanoparticles has emerged as one of the eminent areas to overcome formulation associated problems of the macromolecules.The purpose of the present investigation was to develop protein-loaded nanoparticles using biodegradable polymer poly L-lactide-co-glycolidic acid (PLGA) with bovine serum albumin (BSA) as a model protein.The formulation prepared with protein-polymer ratio of 1:60 at 17,500 rpm gave maximum protein-loading, minimum polydispersion with maximally sustained protein release pattern, among the prepared formulations.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Technology, Jadavpur University, Kolkata, West Bengal, India. biswajit55@yahoo.com

ABSTRACT
Controlled drug delivery technology of proteins/peptides from biodegradable nanoparticles has emerged as one of the eminent areas to overcome formulation associated problems of the macromolecules. The purpose of the present investigation was to develop protein-loaded nanoparticles using biodegradable polymer poly L-lactide-co-glycolidic acid (PLGA) with bovine serum albumin (BSA) as a model protein. Despite many studies available with PLGA-based protein-loaded nanoparticles, production know-how, process parameters, protein loading, duration of protein release, narrowing polydispersity of particles have not been investigated enough to scale up manufacturing of protein-loaded nanoparticles in formulations. Different process parameters such as protein/polymer ratio, homogenizing speed during emulsifications, particle surface morphology and surface charges, particle size analysis and in-vitro protein release were investigated. The in-vitro protein release study suggests that release profile of BSA from nanoparticles could be modulated by changing protein-polymer ratios and/or by varying homogenizing speed during multiple-emulsion preparation technique. The formulation prepared with protein-polymer ratio of 1:60 at 17,500 rpm gave maximum protein-loading, minimum polydispersion with maximally sustained protein release pattern, among the prepared formulations. Decreased (10,000 rpm) or enhanced (24,000 rpm) homogenizing speeds resulted in increased polydispersion with larger particles having no better protein-loading and -release profiles in the present study.

Show MeSH
Related in: MedlinePlus