Limits...
Decreased astroglial cell adhesion and proliferation on zinc oxide nanoparticle polyurethane composites.

Seil JT, Webster TJ - Int J Nanomedicine (2008)

Bottom Line: The surface chemistry was characterized via X-ray photoelectron spectroscopy.Astrocyte adhesion was significantly reduced on ZnO nanoparticle/polyurethane (PU) composites with a weight ratio of 50:50 (PU:ZnO) wt.%, 75:25 (PU:ZnO) wt.%, and 90:10 (PU:ZnO) wt.% in comparison to pure PU.The successful production of ZnO nanoparticle composite scaffolds suitable for decreasing astroglial cell density demonstrates their potential as a nerve guidance channel material with greater efficiency than what may be available today.

View Article: PubMed Central - PubMed

Affiliation: Laboratory for Nanomedicine Research, Division of Engineering, Brown University, Providence, RI 02912, USA.

ABSTRACT
Nanomaterials offer a number of properties that are of interest to the field of neural tissue engineering. Specifically, materials that exhibit nanoscale surface dimensions have been shown to promote neuron function while simultaneously minimizing the activity of cells such as astrocytes that inhibit central nervous system regeneration. Studies demonstrating enhanced neural tissue regeneration in electrical fields through the use of conductive materials have led to interest in piezoelectric materials (or those materials which generate a transient electrical potential when mechanically deformed) such as zinc oxide (ZnO). It has been speculated that ZnO nanoparticles possess increased piezoelectric properties over ZnO micron particles. Due to this promise in neural applications, the objective of the present in vitro study was, for the first time, to assess the activity of astroglial cells on ZnO nanoparticle polymer composites. ZnO nanoparticles embedded in polyurethane were analyzed via scanning electron microscopy to evaluate nanoscale surface features of the composites. The surface chemistry was characterized via X-ray photoelectron spectroscopy. Astroglial cell response was evaluated based on cell adhesion and proliferation. Astrocyte adhesion was significantly reduced on ZnO nanoparticle/polyurethane (PU) composites with a weight ratio of 50:50 (PU:ZnO) wt.%, 75:25 (PU:ZnO) wt.%, and 90:10 (PU:ZnO) wt.% in comparison to pure PU. The successful production of ZnO nanoparticle composite scaffolds suitable for decreasing astroglial cell density demonstrates their potential as a nerve guidance channel material with greater efficiency than what may be available today.

Show MeSH

Related in: MedlinePlus

Results of 1, 2, and 3 day astrocyte proliferation assay. Cells were seeded at t = 0 with 2500 cells/cm2. Values are mean ± SEM; n = 3; *p < 0.05 (compared to 100:0 [PU:ZnO] wt.% at the same time point), **p < 0.05 (compared to 90:10 [PU:ZnO] wt.% at the same time point), ‡p < 0.05 (compared to same sample composition at the 24 h time point), ⋄p < 0.05 (compared to same sample composition at 48 h time point).
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2636581&req=5

f5-ijn-3-523: Results of 1, 2, and 3 day astrocyte proliferation assay. Cells were seeded at t = 0 with 2500 cells/cm2. Values are mean ± SEM; n = 3; *p < 0.05 (compared to 100:0 [PU:ZnO] wt.% at the same time point), **p < 0.05 (compared to 90:10 [PU:ZnO] wt.% at the same time point), ‡p < 0.05 (compared to same sample composition at the 24 h time point), ⋄p < 0.05 (compared to same sample composition at 48 h time point).

Mentions: Lastly, results of this study showed a reduced ability of astrocytes to adhere and proliferate on ZnO nanoparticle PU composites with higher nanoparticle concentrations. Cell images captured after a 4 h adhesion assay illustrated reduced astrocyte cell density as ZnO nanoparticle concentration increased (Figure 3). At 4 h, cell adhesion was significantly reduced on samples with weight ratios of 50:50 (PU:ZnO) wt.%, 75:25 (PU:ZnO) wt.%, and 90:10 (PU:ZnO) wt.% compared to the pure polymer (Figure 4). Increased concentrations of nanoparticles in ZnO/PU composites also reduced cell proliferation. At 24, 48, and 72 h, cell density was reduced on samples with weight ratios of 50:50 (PU: ZnO) wt.%, 75:25 (PU:ZnO) wt.%, and 90:10 (PU:ZnO) wt.% compared to pure polymers at the same time point (Figure 5). At all time points, cell density of samples with weight ratios of 50:50 (PU:ZnO) wt.% and 75:25 (PU:ZnO) wt.% was further reduced compared to composites with weight ratios of 90:10 (PU:ZnO) wt.%. At 72 h, cell density on samples with weight ratios of 50:50 (PU:ZnO) wt.% was reduced compared to cell density on samples of the same composition at the 24 h time point.


Decreased astroglial cell adhesion and proliferation on zinc oxide nanoparticle polyurethane composites.

Seil JT, Webster TJ - Int J Nanomedicine (2008)

Results of 1, 2, and 3 day astrocyte proliferation assay. Cells were seeded at t = 0 with 2500 cells/cm2. Values are mean ± SEM; n = 3; *p < 0.05 (compared to 100:0 [PU:ZnO] wt.% at the same time point), **p < 0.05 (compared to 90:10 [PU:ZnO] wt.% at the same time point), ‡p < 0.05 (compared to same sample composition at the 24 h time point), ⋄p < 0.05 (compared to same sample composition at 48 h time point).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2636581&req=5

f5-ijn-3-523: Results of 1, 2, and 3 day astrocyte proliferation assay. Cells were seeded at t = 0 with 2500 cells/cm2. Values are mean ± SEM; n = 3; *p < 0.05 (compared to 100:0 [PU:ZnO] wt.% at the same time point), **p < 0.05 (compared to 90:10 [PU:ZnO] wt.% at the same time point), ‡p < 0.05 (compared to same sample composition at the 24 h time point), ⋄p < 0.05 (compared to same sample composition at 48 h time point).
Mentions: Lastly, results of this study showed a reduced ability of astrocytes to adhere and proliferate on ZnO nanoparticle PU composites with higher nanoparticle concentrations. Cell images captured after a 4 h adhesion assay illustrated reduced astrocyte cell density as ZnO nanoparticle concentration increased (Figure 3). At 4 h, cell adhesion was significantly reduced on samples with weight ratios of 50:50 (PU:ZnO) wt.%, 75:25 (PU:ZnO) wt.%, and 90:10 (PU:ZnO) wt.% compared to the pure polymer (Figure 4). Increased concentrations of nanoparticles in ZnO/PU composites also reduced cell proliferation. At 24, 48, and 72 h, cell density was reduced on samples with weight ratios of 50:50 (PU: ZnO) wt.%, 75:25 (PU:ZnO) wt.%, and 90:10 (PU:ZnO) wt.% compared to pure polymers at the same time point (Figure 5). At all time points, cell density of samples with weight ratios of 50:50 (PU:ZnO) wt.% and 75:25 (PU:ZnO) wt.% was further reduced compared to composites with weight ratios of 90:10 (PU:ZnO) wt.%. At 72 h, cell density on samples with weight ratios of 50:50 (PU:ZnO) wt.% was reduced compared to cell density on samples of the same composition at the 24 h time point.

Bottom Line: The surface chemistry was characterized via X-ray photoelectron spectroscopy.Astrocyte adhesion was significantly reduced on ZnO nanoparticle/polyurethane (PU) composites with a weight ratio of 50:50 (PU:ZnO) wt.%, 75:25 (PU:ZnO) wt.%, and 90:10 (PU:ZnO) wt.% in comparison to pure PU.The successful production of ZnO nanoparticle composite scaffolds suitable for decreasing astroglial cell density demonstrates their potential as a nerve guidance channel material with greater efficiency than what may be available today.

View Article: PubMed Central - PubMed

Affiliation: Laboratory for Nanomedicine Research, Division of Engineering, Brown University, Providence, RI 02912, USA.

ABSTRACT
Nanomaterials offer a number of properties that are of interest to the field of neural tissue engineering. Specifically, materials that exhibit nanoscale surface dimensions have been shown to promote neuron function while simultaneously minimizing the activity of cells such as astrocytes that inhibit central nervous system regeneration. Studies demonstrating enhanced neural tissue regeneration in electrical fields through the use of conductive materials have led to interest in piezoelectric materials (or those materials which generate a transient electrical potential when mechanically deformed) such as zinc oxide (ZnO). It has been speculated that ZnO nanoparticles possess increased piezoelectric properties over ZnO micron particles. Due to this promise in neural applications, the objective of the present in vitro study was, for the first time, to assess the activity of astroglial cells on ZnO nanoparticle polymer composites. ZnO nanoparticles embedded in polyurethane were analyzed via scanning electron microscopy to evaluate nanoscale surface features of the composites. The surface chemistry was characterized via X-ray photoelectron spectroscopy. Astroglial cell response was evaluated based on cell adhesion and proliferation. Astrocyte adhesion was significantly reduced on ZnO nanoparticle/polyurethane (PU) composites with a weight ratio of 50:50 (PU:ZnO) wt.%, 75:25 (PU:ZnO) wt.%, and 90:10 (PU:ZnO) wt.% in comparison to pure PU. The successful production of ZnO nanoparticle composite scaffolds suitable for decreasing astroglial cell density demonstrates their potential as a nerve guidance channel material with greater efficiency than what may be available today.

Show MeSH
Related in: MedlinePlus